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Journal of Cancer Research and Clinical Oncology

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01.03.2015 | Original Article – Cancer Research

microRNA-548l is involved in the migration and invasion of non-small cell lung cancer by targeting the AKT1 signaling pathway

verfasst von: Caihong Liu, Huan Yang, Zhijie Xu, Dan Li, Meiyu Zhou, Kui Xiao, Zhihui Shi, Lanyan Zhu, Lifang Yang, Rui Zhou

Erschienen in: Journal of Cancer Research and Clinical Oncology | Ausgabe 3/2015

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Abstract

Purpose

microRNAs (miRNAs) have been documented playing a critical role in cancer development and progression. In this study, we investigated the role of miR-548l in non-small cell lung cancer (NSCLC) migration and invasion.

Methods

microRNAs microarray analysis was used to detect the differentially expressed miRNAs between various metastatic levels of NSCLC cells and further confirmed by real-time PCR analysis. To facilitate the delineation of the role of selected miR-548l in NSCLC pathology, we detected its expression in 22 NSCLC tissues. Proliferation, apoptosis, invasion and metastasis effects of the miRNA were evaluated using MTT, flow cytometry, wound healing and invasion assay following transfection with mimics and inhibitors. Luciferase assay and Western blot analysis were performed to assess miR-548l binding to AKT1 gene. AKT1 expression in the clinical tissues was evaluated using immunohistochemical staining.

Results

The results showed a negative relationship between miR-548l expression and lymph node metastasis of NSCLC. Functional assays showed that over-expression of miR-548l suppressed NSCLC cell migration and invasion. Luciferase assays confirmed that miR-548l could directly bind to the 3′ untranslated region of AKT1. Further data showed that the over-expression of AKT1 could rescue the effects of miR-548l in NSCLC cells, and the miR-548l expression was inversely correlated with AKT1 expression in NSCLC tissues. These results indicated that AKT1 was involved in miR-548l-induced suppression of NSCLC cell migration and invasion.

Conclusion

These results suggested that miR-548l may play a causal role through AKT1 in NSCLC invasion and metastasis.

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Titel: microRNA-548l is involved in the migration and invasion of non-small cell lung cancer by targeting the AKT1 signaling pathway
verfasst von: Caihong Liu
Huan Yang
Zhijie Xu
Dan Li
Meiyu Zhou
Kui Xiao
Zhihui Shi
Lanyan Zhu
Lifang Yang
Rui Zhou
Publikationsdatum: 01.03.2015
Verlag: Springer Berlin Heidelberg
Erschienen in: Journal of Cancer Research and Clinical Oncology / Ausgabe 3/2015
Print ISSN: 0171-5216
Elektronische ISSN: 1432-1335
DOI: https://doi.org/10.1007/s00432-014-1836-7

Springer Medizin

microRNA-548l is involved in the migration and invasion of non-small cell lung cancer by targeting the AKT1 signaling pathway