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Digestive Diseases and Sciences

Erschienen in:

10.09.2020 | Original Article

MiR-325 Promotes Oxaliplatin-Induced Cytotoxicity Against Colorectal Cancer Through the HSPA12B/PI3K/AKT/Bcl-2 Pathway

verfasst von: Li Zhang, Heping Chen, Yueqiong Song, Qing Gu, Lu Zhang, Qin Xie, Jin Xu, Min Zhang

Erschienen in: Digestive Diseases and Sciences | Ausgabe 8/2021

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Abstract

Background

Oxaliplatin is one of the most effective chemotherapeutic drugs used for the treatment of colorectal cancer (CRC). However, intervention that attenuates the resistance of oxaliplatin is still required in the treatment of CRC.

Aims

To investigate the role of miR-325 in changing the oxaliplatin sensitivity to CRC cells.

Methods

Expression of miR-325 in colorectal cancer tissues and cell lines was measured by using qRT-PCR analysis. Cytotoxicity of oxaliplatin to control or miR-325-overexpressed HT29 and SW480 cells was evaluated by CCK-8 assays. Luciferase reporter assay was used to confirm the regulation of miR-325 on HSPA12B. Flow cytometry was performed to detect the mitochondrial membrane potential and cell apoptosis.

Results

Expression of miR-325 was decreased in colorectal cancer tissues and cell lines. However, overexpression of miR-325 can decrease the 50% inhibiting concentration of oxaliplatin to colorectal cancer cell lines HT29 and SW480. Mechanically, we confirmed that miR-325 targeted HSPA12B in colorectal cancer. Therefore, overexpression of miR-325 inhibited the phosphorylation of PI3K and AKT and decreased the expression of Bcl-2 to promote the oxaliplatin-induced mitochondrial apoptosis in colorectal cancer.

Conclusions

MiR-325 sensitizes the colorectal cancer cells to oxaliplatin-induced cytotoxicity through the HSPA12B/PI3K/AKT/Bcl-2 pathway.

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Titel: MiR-325 Promotes Oxaliplatin-Induced Cytotoxicity Against Colorectal Cancer Through the HSPA12B/PI3K/AKT/Bcl-2 Pathway
verfasst von: Li Zhang
Heping Chen
Yueqiong Song
Qing Gu
Lu Zhang
Qin Xie
Jin Xu
Min Zhang
Publikationsdatum: 10.09.2020
Verlag: Springer US
Erschienen in: Digestive Diseases and Sciences / Ausgabe 8/2021
Print ISSN: 0163-2116
Elektronische ISSN: 1573-2568
DOI: https://doi.org/10.1007/s10620-020-06579-7

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Background

Aims

Methods

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