Background
Methods
Cell lines and cell culture
Microarray
Quantitative RT-PCR
Immunohistochemical staining of gastric carcinoma tissue
MMP28 overexpressing N87 cells
Western blot analysis
Matrigel chemoinvasion assay
Xenograft assays in nude mice
Statistical analysis
Results
MMP28 is overexpressed in the highly invasive PAMC82-P3 sub-line
MMP28 is overexpressed in gastric carcinoma and correlates with poorer survival
MMP28 expression score | ||||||
---|---|---|---|---|---|---|
Variable | 0 | 1 | 2 | 3 |
P value | |
Age(yrs)
| Mean | 67.0 | 60.8 | 63.7 | 61.0 | 0.374 |
SD | 11.0 | 12.8 | 11.9 | 14.3 | ||
Gender
| Male | 29 | 66 | 70 | 20 | 0.l94 |
Female | 7 | 17 | 24 | 14 | ||
pT1 | 9 | 11 | 5 | 1 | <0.0001* | |
pT (primary tumor)
| pT2 | 21 | 49 | 28 | 8 | |
pT3 | 11 | 25 | 50 | 17 | ||
pT4 | 9 | 8 | 14 | 8 | ||
pN0 | 28 | 19 | 6 | 1 | <0.0001* | |
pN (lymph node metastasis)
| pN1 | 13 | 43 | 26 | 4 | |
pN2 | 9 | 27 | 45 | 12 | ||
pN3 | 0 | 4 | 20 | 17 | ||
pM (distant metastasis)
| pM0 | 49 | 87 | 94 | 30 | 0.154 |
pM1 | 1 | 6 | 3 | 4 |
Variables | Risk ratio (95% CI) |
P value |
---|---|---|
pT (primary tumor)
| 0.693 (0.493-0.974) | 0.035* |
pN (lymph node metastasis)
| 1.254 (0.978-1.608) | 0.044* |
pM (distant metastasis)
| 0.717 (0.349-1.470) | 0.363 |
Score of MMP28 expression
| 1.554 (1.119-2.159) | 0.008* |