Background
Methods
Collection of porcine ovary tissue
Granulosa cell culture
Cloning of MAP1LC3A cDNA
Primer name | Sequence | Size |
---|---|---|
cDNA cloning primers
| ||
GeneRacer 3
′
-primer
|
5
′
GCTGTCAACGATACGCTACGTAACG 3
′
| |
GeneRacer 3
′
-nested primer
|
5
′
CGCTACGTAACGGCATGACAGTG 3
′
| |
GeneRacer 5
′
-primer
|
5
′
CGACTGGAGCACGAGGACACTGA 3
′
| |
GeneRacer 5
′
-nested primer
|
5
′
GGACACTGACATGGACTGAAGGAGTA 3
′
| |
5
′
RACE-Rv primer 1
|
5
′
TGTCCAGGACTGGCAGCTGCTTCT 3
′
| |
5
′
RACE-Rv primer 2
|
5
′
CCAACTCGCTCATGTTGACATGGT 3
′
| |
3
′
RACE-Fw primer
|
5
′
ATCGAGCGCTACAAGGGTGAGAAGC 3
′
| |
Gene expression primers
| ||
Porcine GAPDH Fw
|
5
′
CCCGTTCGACAGACAGCCGTG 3
′
|
238
|
Porcine GAPDH Rv
|
5
′
CCGCCTTGACTGTGCCGTGG 3
′
| |
Porcine MAP1LC3A Fw
|
5
′
AGAAGCAGCTGCCAGTCCTGGACA 3
′
|
687
|
Porcine MAP1LC3A Fw
|
5
′
CAGGCAGGCCTGAGCAATCTTTATT 3
′
| |
Porcine ATG5 Fw
|
5
′
AGAGAAGTCTGTCCTTCCGCAGTCG 3
′
|
241
|
Porcine ATG5 Rv
|
5
′
AAGCAGAAGGGTGACATGCTCTGGT 3
′
|