Erschienen in:
01.07.2010 | Brief Report
Molecular detection of genotype 3 porcine hepatitis E virus in aborted fetuses and their sows
verfasst von:
Myra Hosmillo, Young-Ju Jeong, Hyun-Jeong Kim, Jun-Gyu Park, Mukti Kant Nayak, Mia Madel Alfajaro, Therese Marie Collantes, Su-Jin Park, Kazuyoshi Ikuta, Mikihiro Yunoki, Mun-Il Kang, Sang-Ik Park, Kyoung-Oh Cho
Erschienen in:
Archives of Virology
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Ausgabe 7/2010
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Abstract
By reverse transcription (RT)-nested polymerase chain reaction (PCR), hepatitis E virus (HEV) RNA was simultaneously detected in the livers of aborted fetuses and in fecal and serum samples from their sows, obtained from two of 14 farms tested. Sequence and phylogenetic analyses showed that these HEVs belonged to genotype 3 HEV. HEV RNA was detected by real-time RT-PCR at 2.0 × 103–2.8 × 104 viral copies/μl in the HEV-positive samples. All HEV-positive fetuses tested positive for porcine circovirus type 2 (PCV2). These results indicate that transplacental infection of HEV with PCV2 coinfection may occur in sows with reproductive failure, which is suggestive of similarities to HEV infection in women.