Erschienen in:
25.01.2017 | Original Article
Molecular identification of Mecistocirrus digitatus and Toxocara vitulorum in the mithun (Bos frontalis) from north-east India
verfasst von:
Jayanta Kumar Chamuah, O. K. Raina, A. Sakhrie, N. Gama
Erschienen in:
Journal of Parasitic Diseases
|
Ausgabe 3/2017
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Abstract
Mecistocirrus digitatus and Toxocara vitulorum are common pathogenic nematode parasites of mithun (Bos frontalis). Species identification by morphological features was confirmed by molecular identification of M. digitatus and T. vitulorum. The internal transcribed spacer-2 (ITS-2) region and beta tubulin gene of M. digitatus were polymerase chain reaction (PCR) amplified and sequenced. ITS-2 sequence analysis showed 100% homology with other isolates of M. digitatus and 83% identity with Haemonchus contortus and H. placei, respectively. Likewise, ITS-1 and ITS-2 sequences of T. vitulorum were PCR amplified and sequenced. Sequence analysis of these internal transcribed spacers from five worms of the parasite from mithun showed no intraspecific variations with T. vitulorum isolates from domestic ruminants.