Introduction
Sepsis is a dysregulated host response to infection that affects millions of people worldwide each year [
1]. Its mortality rate ranges from 16.67 to 33.33% [
2]. Timely assessment and appropriate treatment during the initial hours of sepsis can improve outcomes [
3]. The leading cause of sepsis-related deaths between 1990 and 2017 was infection [
4]. Conditions like cytokine storms and sepsis-induced immunosuppression increase sepsis severity and induce poor prognosis [
5]. Monitoring the immune status of patients with sepsis and providing immunomodulatory treatment may improve survival rates [
6]. Some biomarkers have been reported to predict sepsis in infection; however, the identification of further suitable immune indicators to assess the host immune status is vital for early diagnosis and better prognostic power.
Low-density neutrophils (LDNs) are found in peripheral blood mononuclear cells (PBMCs) after density gradient centrifugation of whole blood [
7]. Depending on their function, LDNs are characterized as immunosuppressive, proinflammatory, or immature neutrophils
[7]. LDN counts are typically small in healthy donors [
8], but are elevated in alcohol-associated hepatitis [
9],
Mycobacterium tuberculosis infection [
10], systemic lupus erythematosus (SLE) [
11], severe obesity [
12], myocardial infarction [
13] and abdominal surgery [
14]. Myeloid-derived suppressor cells (MDSCs) are pathologically activated neutrophils and monocytes with immunosuppressive activities [
15]. Based on their origin, MDSCs are classified into two major groups: granulocytic/polymorphonuclear MDSCs (PMN-MDSCs) and monocytic MDSCs (M-MDSCs) [
15]. M-MDSCs are defined as CD11b
+CD14
+HLA-DR
−/loCD15
− cells and PMN-MDSCs as CD11b
+CD14
−CD15
+ cells in human PBMCs [
16]. MDSCs, which are widely recognized for their ability to attenuate immune surveillance and their antitumor properties, play a role in deleterious immunosuppressive processes.
Immunotherapy can normalize immune cells and improve the prognosis of patients. Thus, we explored the possibility of using immune cells as biomarkers for sepsis. We assessed whether the levels of immunosuppressive indicators, LDNs and MDSC subsets in PBMCs, differ in patients with infection or sepsis. In addition, we evaluated the association between immune cells and the severity of sepsis to find a potential prognostic biomarker of sepsis.
Discussion
An excessive pro-inflammatory response may result in tissue damage and organ failure in patients with sepsis. Concurrently, persistent anti-inflammatory responses promote sustained immunosuppressive environments [
17]. Sepsis-induced immunosuppression is a critical factor in sepsis mortality [
6]. Providing direct evidence of the severity of the infection and the immune status of patients is vital. PCT and CRP were the most frequently studied biomarkers in patients with infections between 2009 and 2019 [
18]. We observed that the LDNs and MDSC subsets were positively correlated with CRP and PCT levels, suggesting that they could also be potential infection biomarkers.
Neutrophils play a protective role in the immune response to invading pathogens [
19]. Unlike normal neutrophils, LDNs show obvious abnormal immunological functions, evidenced by myelocyte-like or band-shaped nuclei, immature granulocytes, multilobed nuclei, and mature neutrophils [
20]. LDNs were first separated using Ficoll-Hypaque gradients and first identified in PBMCs of patients with SLE, rheumatoid arthritis, and acute rheumatic fever [
21]. Our results showed that patients with infection or sepsis had higher LDN levels than healthy controls (HCs). This result is similar to another study in which LDN levels were higher in 26 patients with sepsis admitted to the intensive care unit (ICU) than those in 15 HCs [
20].
With regards to infection sites, LDN levels were higher in the local-bloodstream mixed infection group than those in the local infection group. LDNs can distinguish infections in bloodstream from local infections. In our study, LDNs were also elevated in bacterial infections, whereas LDN levels were normal in viral infections. Therefore, LDNs have the potential to accurately distinguish bacterial from viral infections. This is a similar finding to a previous study, in which a large quantity of LDNs was found in the peripheral blood of patients with bacterial infections [
22]. LDNs are associated with a higher incidence of secondary infections. In one study, high initial levels of LDNs were associated with a higher risk of secondary nosocomial infections among patients with sepsis [
23], likely because LDNs produce high levels of mitochondrial reactive oxygen species (ROS) and neutrophil extracellular traps (NETs) [
9,
24,
25]. Spontaneous NETs contain elevated levels of oxidized mitochondrial DNA, which can lead to the synthesis of IFN-β, promote endothelial damage and vasculopathy, activate platelets, and potentially promote thrombosis [
26].
M-MDSCs and PMN-MDSCs are pathologically activated neutrophils and monocytes [
15]. Therefore, we analyzed MDSC subsets according to their origin. We observed that the monocyte counts were higher in the infection and sepsis groups than in the HCs group, but there was no significant difference between the infection and sepsis groups. The percentage of monocytes was highest in the infection group, followed by the healthy controls, and finally the sepsis group. The neutrophil count and percentage, MLR, and NLR gradually increased in the HCs, infection, and sepsis groups. This supports our finding that mature and immature neutrophil levels were proportionally increased in sepsis compared to those in HCs, whereas monocyte levels were decreased [
27].
MDSC-like cells (MDSC-LC) can be screened based on their phenotype. MDSCs-LC with immunosuppressive function can be identified as MDSCs [
16,
28]. We did not test and verify the function of MDSCs. Still, our study exhibited M-MDSCs and PMN-MDSCs levels were lowest in the HCs group, elevated in the infection group, and highest in the sepsis group. Mathias et al. have mentioned MDSCs increased rapidly and permanently in patients hospitalized in the ICU for more than 28 days [
29]. Furthermore, genes associated with MDSCs recruitment, phenotype, and suppressive functions were upregulated, whereas those associated with adaptive immunity and inflammation were downregulated in a cohort of 29 patients with sepsis and 15 healthy donors [
23]. Uhel et al. demonstrated that M-MDSCs were increased in ICU patients with and without sepsis and that CD14
−CD15
+ low-density PMN-MDSCs were specifically increased in patients with sepsis [
23].
In subgroup analysis of infection sites, among 63 local infections, 8 bloodstream infections, and 14 bloodstream-local mixed infection group, we found M-MDSCs were lower in bloodstream infections than those in mixed infections, whereas PMN-MDSCs were lower in local infections than those in bloodstream-local mixed infections. Analysis of infectious pathogens subgroup showed that M-MDSCs play a key role in the identification of fungal and viral infections, whereas PMN-MDSCs play a major role in the identification of fungal and bacterial infections. In addition, both M-MDSCs and PMN-MDSCs levels were higher in bacterial infections compared to those in viral infections. This result is consistent with that of a study discovered the number of circulating CD14
+HLA-DR
lo/− M-MDSCs was higher in patients with gram-negative sepsis than in those with gram-positive sepsis [
23].
The SOFA score is associated with the severity of organ dysfunction and indicates an increased probability of mortality in patients with sepsis [
1]. The LDNs and MDSC subsets were positively associated with the SOFA score and the number of dysfunctional organs, suggesting that they could be potential prognostic biomarkers of sepsis. High initial levels of PMN-MDSCs are associated with a higher risk of secondary nosocomial infections among patients with sepsis. A previous study demonstrated that M-MDSCs were not significantly different between 9 patients with nosocomial infection and 27 patients without nosocomial infection [
23]; however, we found both PMN-MDSCs and M-MDSCs were associated with a higher incidence of secondary infections. This difference in result could be due to the number of participants in the study. Here, we analyzed 24 and 61 patients with and without secondary infections, respectively. Moreover, our study demonstrated the percentage of M-MDSCs in PBMCs instead of in whole blood. Despite M-MDSCs levels not differing significantly between septic patients with and without shock, MDSCs were higher in the shock subgroup than in the non-shock subgroup. This might be because the number of patients with sepsis was too small to produce statistical evidence.
Patients who died early had the highest initial percentage of MDSCs at 12h and 24 h [
29]. For example, in an animal study, T cell proliferation was reduced by 75% in the presence of MDSCs derived from septic mice and by 40% in the presence of MDSCs from uninfected controls, demonstrating that MDSCs from septic neonates were more suppressive [
30]. In humans, MDSCs from patients with sepsis significantly suppress the ability of healthy control T cells to produce IFN-γ and IL-4 cytokines [
29]. Zhao et al. found that MDSCs secrete itaconate to suppress CD8
+ T cells proliferation, cytokine production and cytotoxicity [
31]. Hollen et al. demonstrated that MDSCs significantly increased in hospitalized sepsis survivors at least 6 weeks after infection. However, MDSCs isolated from sepsis exhibit immunosuppressive function by inhibiting T cells proliferation and IL-2 production only at or after 14 days following sepsis onset [
32]. MDSCs not only suppress the number and function of T cells, but also enhance the number of Treg [
33], and produce cytokines with immunosuppressive function, such as IL-10 [
34] and TGF-β [
35], impacting the prognosis of patients with sepsis.
There was no research on the prognosis of sepsis by use of LDNs and MDSCs. The only one report about the prognostic value of M-MDSCs in sepsis was published by Schrijver et al. in 2022. It showed that survivors expressed 1.64-fold more M-MDSCs than 28-day non-survivors in pneumosepsis [
36]. In our study, LDNs and MDSCs were positively associated with sepsis severity. Therefore, we evaluated their prognostic value in survivors and non-survivors. M-MDSC levels were higher in non-survivors than in survivors, and Cox regression analysis demonstrated that M-MDSC was an independent predictor for 28-day mortality. This may be one of the factors related to the prognostic value of M-MDSCs in sepsis, when CD14
+ cells (M-MDSCs) were depleted, CD4
+ and CD8
+ T cells in patients with sepsis proliferated rapidly [
23].
Our results illustrate that PCT levels did not differ between sepsis survivors and non-survivors. However, Cox regression analysis demonstrated that PCT was associated with a high 28-day mortality rate in patients with sepsis. Through an analysis of 249 patients who were suspected of having sepsis in the emergency department, Lee et al. also reported that PCT was not an independent predictor for mortality [
37]. Manifold studies have been published on PCT in sepsis, with some conflicting results on whether PCT can predict prognosis in patients with sepsis. Comparison of 159 survivors and 26 non-survivors of sepsis with suspected bacterial infections showed that PCT could predict poor prognosis of sepsis [
38], in agreement with the results of Yang [
39] and Mustafic [
40]. This difference may be related to the number of study participants and the types of pathogens, even the infection sites causing sepsis.
Our study had some limitations. First, LDNs and MDSCs were isolated using density gradient centrifugation, making it difficult to integrate them into routine clinical practice. Second, we did not continuously monitor LDNs and MDSC subsets to determine their dynamic changes. Furthermore, studies are needed to examine a larger number of samples and patients from another research center to increase the diversity of the patient cohorts.
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