nach oben

Archives of Virology

Erschienen in:

01.10.2014 | Brief Report

Mutational modifications of hepatitis A virus proteins 2B and 2C described for cell culture-adapted and attenuated virus are present in wild-type virus populations

verfasst von: Rebecca Weilandt, Dajana Paulmann, Kore Schlottau, Angelika Vallbracht, Andreas Dotzauer

Erschienen in: Archives of Virology | Ausgabe 10/2014

Einloggen, um Zugang zu erhalten

Abstract

Studies have identified certain mutations in the 2B and 2C proteins of hepatitis A virus (HAV) as being essential for efficient growth in cultured cells, and it is assumed that these mutations contribute to the attenuated phenotype. We found that mutations supporting cell culture growth already exist in wild-type HAV populations. This suggests that these variants are not entirely generated de novo but are selected from the wild-type population. In a prolonged case of hepatitis A, we found that sequences associated with cell culture adaptation predominated later in infection. This might suggest selection of an attenuated virus population during a prolonged clinical infection.

Dotzauer A (2008) Hepatitis A Virus. In: Mahy BWJ, Van Regenmortel MHV (eds) Encyclopedia of virology. Elsevier, Oxford, pp 343–350CrossRef

Dotzauer A, Kraemer L (2012) Innate and adaptive immune responses against picornaviruses and their counteractions: an overview. World J Virol 1(3):91–107PubMedCrossRefPubMedCentral

Frösner GG, Deinhardt F, Scheid R, Gauss-Müller V, Holmes N, Messelberger V, Siegl G, Alexander JJ (1979) Propagation of human hepatitis A virus in a hepatoma cell line. Infection 7:303–305PubMedCrossRef

Provost PJ, Hilleman MR (1979) Propagation of human hepatitis A virus in cell culture in vitro. Proc Soc Exp Biol Med 160:213–221PubMedCrossRef

Flehmig B (1980) Hepatitis A-virus in cell culture: I. propagation of different hepatitis A-virus isolates in a fetal rhesus monkey kidney cell line (Frhk-4). Med Microbiol Immunol 168:239–248PubMedCrossRef

Flehmig B, Vallbracht A, Wurster G (1981) Hepatitis A virus in cell culture. III. Propagation of hepatitis A virus in human embryo kidney cells and human embryo fibroblast strains. Med Microbiol Immunol 170:83–89PubMedCrossRef

Gauss-Müller V, Frösner GG, Deinhardt F (1981) Propagation of hepatitis A virus in human embryo fibroblasts. J Med Virol 7:233–239PubMedCrossRef

Daemer RJ, Feinstone SM, Gust ID, Purcell RH (1981) Propagation of human hepatitis A virus in African green monkey kidney cell culture: primary isolation and serial passage. Infect Immun 32:388–393PubMedPubMedCentral

Binn LN, Lemon SM, Marchwicki RH, Redfield RR, Gates NL, Bancroft WH (1984) Primary isolation and serial passage of hepatitis A virus strains in primate cell cultures. J Clin Microbiol 20:28–33PubMedPubMedCentral

10.

Vallbracht A, Hofmann L, Wurster KG, Flehmig B (1984) Persistent infection of human fibroblasts by hepatitis A virus. J Gen Virol 65:609–615PubMedCrossRef

11.

De Chastonay J, Siegl G (1987) Replicative events in hepatitis A virus-infected MRC-5 cells. Virology 157:268–275PubMedCrossRef

12.

Dotzauer A, Feinstone SM, Kaplan G (1994) Susceptibility of nonprimate cell lines to hepatitis A virus infection. J Virol 68:6064–6068PubMedPubMedCentral

13.

Cohen JI, Rosenblum B, Ticehurst JR, Daemer RJ, Feinstone SM, Purcell RH (1987) Complete nucleotide sequence of an attenuated hepatitis A virus: comparison with wild-type virus. Proc Natl Acad Sci USA 84:2497–2501PubMedCrossRefPubMedCentral

14.

Cohen JI, Ticehurst JR, Feinstone SM, Rosenblum B, Purcell RH (1987) Hepatitis A virus cDNA and its RNA transcripts are infectious in cell culture. J Virol 61:3035–3039PubMedPubMedCentral

15.

Jansen RW, Newbold JE, Lemon SM (1988) Complete nucleotide sequence of a cell culture-adapted variant of hepatitis A virus: comparison with wild-type virus with restricted capacity for in vitro replication. Virology 163:299–307PubMedCrossRef

16.

Cohen JI, Rosenblum B, Feinstone SM, Ticehurst JR, Purcell RH (1989) Attenuation and cell culture adaptation of hepatitis A virus (HAV): a genetic analysis with HAV cDNA. J Virol 63:5364–5370PubMedPubMedCentral

17.

Ross BC, Anderson BN, Edwards PC, Gust ID (1989) Nucleotide sequence of high-passage hepatitis A virus strain HM175: comparison with wild-type and cell culture-adapted strains. J Gen Virol 70:2805–2810PubMedCrossRef

18.

Day SP, Lemon SM (1990) A single base mutation in the 5’ noncoding region of HAV enhances replication of virus in vitro. Cold Spring Harbor Laboratory Press, New York

19.

Emerson SU, McRill C, Rosenblum B, Feinstone SM, Purcell RH (1991) Mutations responsible for adaptation of hepatitis A virus to efficient growth in cell culture. J Virol 65:4882–4886PubMedPubMedCentral

20.

Day SP, Murphy P, Brown EA, Lemon SM (1992) Mutations within the 5’ nontranslated region of hepatitis A virus RNA which enhance replication in BS-C-1 cells. J Virol 66:6533–6540PubMedPubMedCentral

21.

Emerson SU, Huang YK, McRill C, Lewis M, Purcell RH (1992) Mutations in both the 2B and 2C genes of hepatitis A virus are involved in adaptation to growth in cell culture. J Virol 66:650–654PubMedPubMedCentral

22.

Emerson SU, Huang YK, Purcell RH (1993) 2B and 2C mutations are essential but mutations throughout the genome of HAV contribute to adaptation to cell culture. Virology 194:475–480PubMedCrossRef

23.

Tedeschi V, Purcell RH, Emerson SU (1993) Partial characterization of hepatitis A viruses from three intermediate passage levels of a series resulting in adaptation to growth in cell culture and attenuation of virulence. J Med Virol 39:16–22PubMedCrossRef

24.

Graff J, Normann A, Feinstone SM, Flehmig B (1994) Nucleotide sequence of wild-type hepatitis A virus GBM in comparison with two cell culture-adapted variants. J Virol 68:548–554PubMedPubMedCentral

25.

Funkhouser AW, Purcell RH, D’Hondt E, Emerson SU (1994) Attenuated hepatitis A virus: genetic determinants of adaptation to growth in MRC-5 cells. J Virol 68:148–157PubMedPubMedCentral

26.

Funkhouser AW, Raychaudhuri G, Purcell RH, Govindarajan S, Elkins R, Emerson SU (1996) Progress toward the development of a genetically engineered attenuated hepatitis A virus vaccine. J Virol 70:7948–7957PubMedPubMedCentral

27.

Schultz DE, Honda M, Whetter LE, McKnight KL, Lemon SM (1996) Mutations within the 5’ nontranslated RNA of cell culture-adapted hepatitis A virus which enhance cap-independent translation in cultured African green monkey kidney cells. J Virol 70:1041–1049PubMedPubMedCentral

28.

Graff J, Normann A, Flehmig B (1997) Influence of the 5’ noncoding region of hepatitis A virus strain GBM on its growth in different cell lines. J Gen Virol 78:1841–1849PubMed

29.

Raychaudhuri G, Govindarajan S, Shapiro M, Purcell RH, Emerson SU (1998) Utilization of chimeras between human (HM-175) and simian (AGM-27) strains of hepatitis A virus to study the molecular basis of virulence. J Virol 72:7467–7475PubMedPubMedCentral

30.

Frings W, Dotzauer A (2001) Adaptation of primate cell-adapted hepatitis A virus strain HM175 to growth in guinea pig cells is independent of mutations in the 5’ nontranslated region. J Gen Virol 82:597–602PubMed

31.

Emerson SU, Huang YK, Nguyen H, Brockington A, Govindarajan S, St Claire M, Shapiro M, Purcell RH (2002) Identification of VP1/2A and 2C as virulence genes of hepatitis A virus and demonstration of genetic instability of 2C. J Virol 76:8551–8559PubMedCrossRefPubMedCentral

32.

Graff J, Emerson SU (2003) Importance of amino acid 216 in nonstructural protein 2B for replication of hepatitis A virus in cell culture and in vivo. J Med Virol 71:7–17PubMedCrossRef

33.

Ticehurst JR, Racaniello VR, Baroudy BM, Baltimore D, Purcell RH, Feinstone SM (1983) Molecular cloning and characterization of hepatitis A virus cDNA. Proc Natl Acad Sci USA 80:5885–5889PubMedCrossRefPubMedCentral

34.

Teterina NL, Bienz K, Egger D, Gorbalenya AE, Ehrenfeld E (1997) Induction of intracellular membrane rearrangements by HAV proteins 2C and 2BC. Virology 237:66–77PubMedCrossRef

35.

Jecht M, Probst C, Gauss-Müller V (1998) Membrane permeability induced by hepatitis A virus proteins 2B and 2BC and proteolytic processing of HAV 2BC. Virology 252:218–227PubMedCrossRef

36.

Gosert R, Egger D, Bienz K (2000) A cytopathic and a cell culture adapted hepatitis A virus strain differ in cell killing but not in intracellular membrane rearrangements. Virology 266:157–169PubMedCrossRef

37.

de Jong AS, de Mattia F, Van Dommelen MM, Lanke K, Melchers WJ, Willems PH, van Kuppeveld FJ (2008) Functional analysis of picornavirus 2B proteins: effects on calcium homeostasis and intracellular protein trafficking. J Virol 82:3782–3790PubMedCrossRefPubMedCentral

38.

Paulmann D, Magulski T, Schwarz R, Heitmann L, Flehmig B, Vallbracht A, Dotzauer A (2008) Hepatitis A virus protein 2B suppresses beta interferon (IFN) gene transcription by interfering with IFN regulatory factor 3 activation. J Gen Virol 89:1593–1604PubMedCrossRef

39.

Gust ID, Feinstone SM (1988) Hepatitis A. CRC Press, Boca Raton, p 95

40.

Dotzauer A, Heitmann A, Laue T, Kraemer L, Schwabe K, Paulmann D, Flehmig B, Vallbracht A (2012) The role of immunoglobulin A in prolonged and relapsing hepatitis A virus infections. J Gen Virol 93:754–760PubMedCrossRef

Titel: Mutational modifications of hepatitis A virus proteins 2B and 2C described for cell culture-adapted and attenuated virus are present in wild-type virus populations
verfasst von: Rebecca Weilandt
Dajana Paulmann
Kore Schlottau
Angelika Vallbracht
Andreas Dotzauer
Publikationsdatum: 01.10.2014
Verlag: Springer Vienna
Erschienen in: Archives of Virology / Ausgabe 10/2014
Print ISSN: 0304-8608
Elektronische ISSN: 1432-8798
DOI: https://doi.org/10.1007/s00705-014-2103-6

Leitlinien kompakt für die Innere Medizin

Mit medbee Pocketcards sicher entscheiden.

^{Seit 2022 gehört die medbee GmbH zum Springer Medizin Verlag}

Kostenlos registrieren

Neu im Fachgebiet Innere Medizin

Hodgkin Lymphom: BrECADD-Regime übertrifft die Erwartungen

05.06.2024 ASCO 2024 Kongressbericht

Das Kombinationsregime BrECADD mit Brentuximab vedotin ermöglichte in der Studie HD21 beim fortgeschrittenen klassischen Hodgkin-Lymphom eine unerwartet hohe progressionsfreie Überlebensrate von 94,3% nach vier Jahren. Gleichzeitig war das Regime besser tolerabel als der bisherige Standard eBEACOPP.

Antikörper-Drug-Konjugat verdoppelt PFS bei Multiplem Myelom

05.06.2024 ASCO 2024 Nachrichten

Zwei Phase-3-Studien deuten auf erhebliche Vorteile des Antikörper-Wirkstoff-Konjugats Belantamab-Mafodotin bei vorbehandelten Personen mit Multiplem Myelom: Im Vergleich mit einer Standard-Tripeltherapie wurde das progressionsfreie Überleben teilweise mehr als verdoppelt.

Neuer TKI gegen CML: Höhere Wirksamkeit, seltener Nebenwirkungen

05.06.2024 Chronische myeloische Leukämie Nachrichten

Der Tyrosinkinasehemmer (TKI) Asciminib ist älteren Vertretern dieser Gruppe bei CML offenbar überlegen: Personen mit frisch diagnostizierter CML entwickelten damit in einer Phase-3-Studie häufiger eine gut molekulare Response, aber seltener ernste Nebenwirkungen.

Hereditäres Angioödem: Tablette könnte Akuttherapie erleichtern

05.06.2024 Hereditäres Angioödem Nachrichten

Medikamente zur Bedarfstherapie bei hereditärem Angioödem sind bisher nur als Injektionen und Infusionen verfügbar. Der Arzneistoff Sebetralstat kann oral verabreicht werden und liefert vielversprechende Daten.

Update Innere Medizin

Bestellen Sie unseren Fach-Newsletter und bleiben Sie gut informiert.

Newsletter bestellen

Springer Medizin

Abstract

Bitte loggen Sie sich ein, um Zugang zu diesem Inhalt zu erhalten

Weitere Artikel der Ausgabe 10/2014

Eastern equine encephalitis cases among horses in Brazil between 2005 and 2009

Complete sequence of a novel duck astrovirus

Immune and inflammatory response in pigs during acute influenza caused by H1N1 swine influenza virus

A vesicular stomatitis pseudovirus expressing the surface glycoproteins of influenza A virus

Changes to the Statutes and Subcommittees of the International Committee on Taxonomy of Viruses (2014)