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07.09.2019 | Original Contribution

NAD⁺ precursor increases aerobic performance in mice

verfasst von: Barbara M. Crisol, Camilla B. Veiga, Renata R. Braga, Luciene Lenhare, Igor L. Baptista, Rafael C. Gaspar, Vitor R. Muñoz, André V. Cordeiro, Adelino S. R. da Silva, Dennys E. Cintra, Leandro P. Moura, José R. Pauli, Eduardo R. Ropelle

Erschienen in: European Journal of Nutrition | Ausgabe 6/2020

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Abstract

Purpose

Nicotinamide riboside (NR) acts as a potent NAD⁺ precursor and improves mitochondrial oxidative capacity and mitochondrial biogenesis in several organisms. However, the effects of NR supplementation on aerobic performance remain unclear. Here, we evaluated the effects of NR supplementation on the muscle metabolism and aerobic capacity of sedentary and trained mice.

Methods

Male C57BL/6 J mice were supplemented with NR (400 mg/Kg/day) over 5 and 10 weeks. The training protocol consisted of 5 weeks of treadmill aerobic exercise, for 60 min a day, 5 days a week. Bioinformatic and physiological assays were combined with biochemical and molecular assays to evaluate the experimental groups.

Results

NR supplementation by itself did not change the aerobic performance, even though 5 weeks of NR supplementation increased NAD⁺ levels in the skeletal muscle. However, combining NR supplementation and aerobic training increased the aerobic performance compared to the trained group. This was accompanied by an increased protein content of NMNAT3, the rate-limiting enzyme for NAD + biosynthesis and mitochondrial proteins, including MTCO1 and ATP5a. Interestingly, the transcriptomic analysis using a large panel of isogenic strains of BXD mice confirmed that the Nmnat3 gene in the skeletal muscle is correlated with several mitochondrial markers and with different phenotypes related to physical exercise. Finally, NR supplementation during aerobic training markedly increased the amount of type I fibers in the skeletal muscle.

Conclusion

Taken together, our results indicate that NR may be an interesting strategy to improve mitochondrial metabolism and aerobic capacity.

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Titel: NAD+ precursor increases aerobic performance in mice
verfasst von: Barbara M. Crisol
Camilla B. Veiga
Renata R. Braga
Luciene Lenhare
Igor L. Baptista
Rafael C. Gaspar
Vitor R. Muñoz
André V. Cordeiro
Adelino S. R. da Silva
Dennys E. Cintra
Leandro P. Moura
José R. Pauli
Eduardo R. Ropelle
Publikationsdatum: 07.09.2019
Verlag: Springer Berlin Heidelberg
Erschienen in: European Journal of Nutrition / Ausgabe 6/2020
Print ISSN: 1436-6207
Elektronische ISSN: 1436-6215
DOI: https://doi.org/10.1007/s00394-019-02089-z

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NAD⁺ precursor increases aerobic performance in mice

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