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Journal of Natural Medicines

Erschienen in:

11.05.2018 | Original Paper

New podolactones from the seeds of Podocarpus nagi and their anti-inflammatory effect

verfasst von: Zhe-Ling Feng, Tian Zhang, Jing-Xin Liu, Xiu-Ping Chen, Li-She Gan, Yang Ye, Li-Gen Lin

Erschienen in: Journal of Natural Medicines | Ausgabe 4/2018

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Abstract

Podolactones are a class of structural diverse diterpenoid lactones, mainly isolated from the Podocarpus species. Several bioactivities have been disclosed for podolactones, including cytotoxicity and anti-atherosclerosis. In this study, the seeds of P. nagi were isolated by comprehensive chromatographic methods to obtain three new podolatones, named nagilactone B 1-O-β-d-glucoside (1), nagilactone N3 3-O-β-d-glucoside (2), and 2-epinagilactone B (3), as well as a known compound, nagilactone B (4). Their structures were determined by analyses of NMR and HRESIMS data. Compounds 1 and 2 significantly inhibited nitric oxide (NO) production on LPS-stimulated RAW264.7 macrophages, with IC₅₀ values of 0.18 ± 0.04 and 0.53 ± 0.03 μM, respectively. Indomethacin (IC₅₀ 4.21 ± 0.32 μM) was used as a positive control. Compound 1 suppressed the expression of inducible NO synthase (iNOS) in a concentration-dependent manner, mediating through inhibiting nuclear factor-κB (NF-κB) activity. This is the first report regarding the anti-inflammatory effect of podolactones, which could be potential anti-inflammatory agents.

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Titel: New podolactones from the seeds of Podocarpus nagi and their anti-inflammatory effect
verfasst von: Zhe-Ling Feng
Tian Zhang
Jing-Xin Liu
Xiu-Ping Chen
Li-She Gan
Yang Ye
Li-Gen Lin
Publikationsdatum: 11.05.2018
Verlag: Springer Japan
Erschienen in: Journal of Natural Medicines / Ausgabe 4/2018
Print ISSN: 1340-3443
Elektronische ISSN: 1861-0293
DOI: https://doi.org/10.1007/s11418-018-1219-5

Springer Medizin

Abstract

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