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01.01.2015 | Original Article

Nhumirim virus, a novel flavivirus isolated from mosquitoes from the Pantanal, Brazil

verfasst von: Alex Pauvolid-Corrêa, Owen Solberg, Dinair Couto-Lima, Joan Kenney, Nicolau Serra-Freire, Aaron Brault, Rita Nogueira, Stanley Langevin, Nicholas Komar

Erschienen in: Archives of Virology | Ausgabe 1/2015

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Abstract

We describe the isolation of a novel flavivirus, isolated from a pool of mosquitoes identified as Culex (Culex) chidesteri collected in 2010 in the Pantanal region of west-central Brazil. The virus is herein designated Nhumirim virus (NHUV) after the name of the ranch from which the mosquito pool was collected. Flavivirus RNA was detected by real-time RT-PCR of homogenized mosquitoes and from the corresponding C6/36 culture supernatant. Based on full-genome sequencing, the virus isolate was genetically distinct from but most closely related to Barkedji virus (BJV), a newly described flavivirus from Senegal. Phylogenetic analysis demonstrated that NHUV grouped with mosquito-borne flaviviruses forming a clade with BJV. This clade may be genetically intermediate between the Culex-borne flaviviruses amplified by birds and the insect-only flaviviruses.

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Titel: Nhumirim virus, a novel flavivirus isolated from mosquitoes from the Pantanal, Brazil
verfasst von: Alex Pauvolid-Corrêa
Owen Solberg
Dinair Couto-Lima
Joan Kenney
Nicolau Serra-Freire
Aaron Brault
Rita Nogueira
Stanley Langevin
Nicholas Komar
Publikationsdatum: 01.01.2015
Verlag: Springer Vienna
Erschienen in: Archives of Virology / Ausgabe 1/2015
Print ISSN: 0304-8608
Elektronische ISSN: 1432-8798
DOI: https://doi.org/10.1007/s00705-014-2219-8

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