Background
Methods
Patients
BPH (n = 6) | OCPCa (n = 6) | EPCa (n = 6) |
P
| |
---|---|---|---|---|
Age (years) | 67.4 ± 3.9 | 59.4 ± 2.9 | 66.9 ± 2.3 | 0.140a
|
BMI (kg/m2) | 26.5 ± 1.5 | 25.2 ± 1.3 | 26.5 ± 1.6 | 0.783a
|
Serum total PSA (ng/mL) | 8.9 ± 4.7 | 6.7 ± 1.6 | 14.7 ± 3.0 | 0.114b
|
Prostate weight (g) | 107.0 ± 12.9 | 40.8 ± 4.2 | 71.2 ± 9.5 | 0.001a, c
|
Serum leptin (mg/mL) | 3.7 ± 1.0 | 7.1 ± 2.5 | 4.9 ± 1.8 | 0.453a
|
Combined Gleason score | ||||
≤7 (3+4), n (%) | --- | 5 (83%) | 4 (67%) | 1.000d
|
≥7 (4+3), n (%) | --- | 1 (17%) | 2 (33%) |
RNA extraction, microarray hybridization and data processing
Enrichment analysis using Ingenuity Pathway Analysis software
Real-Time PCR
Plasma leptin measurement
Statistical analysis
Results
Patient characteristics
PCA3 gene expression in PP adipose tissue
Defining the PP adipose tissue gene expression signature in OB/OW subjects
Probe set | Gene Name | Gene Description | Fold Change |
P
|
---|---|---|---|---|
All subjects | ||||
8098146 |
NPY5R
| neuropeptide Y receptor Y5 | 1.80 | 1.49E-05 |
7948612 |
FADS1
| fatty acid desaturase 1 | -1.73 | 1.16E-04 |
8103494 |
NPY1R
| neuropeptide Y receptor Y1 | 1.97 | 1.56E-04 |
7969050 |
CYSLTR2
| cysteinyl leukotriene receptor 2 | 1.89 | 2.19E-04 |
8073633 |
PNPLA3
| patatin-like phospholipase domain containing 3 | -1.56 | 5.74E-04 |
7930181 |
AS3MT
| arsenic (+3 oxidation state) methyltransferase | 1.53 | 6.54E-04 |
BPH | ||||
8020343 |
ANKRD20A5
| ankyrin repeat domain 20 family; member A5 | -1.51 | 3.95E-05 |
8152297 |
ANGPT1
| angiopoietin 1 | 1.63 | 2.08E-04 |
7937508 |
CD151
| CD151 molecule (Raph blood group) | 1.69 | 2.43E-04 |
7959102 |
HSPB8
| heat shock 22kDa protein 8 | 1.51 | 3.88E-04 |
8047780 |
SNORA41
| small nucleolar RNA; H/ACA box 41 | -1.77 | 4.94E-04 |
8098146 |
NPY5R
| neuropeptide Y receptor Y5 | 2.06 | 7.86E-04 |
8135909 |
LEP
| leptin | 1.95 | 8.00E-04 |
OCPCa | ||||
7997239
|
PDXDC2
|
pyridoxal-dependent decarboxylase domain containing 2
|
1.99
|
8.30E-06
|
7994026 |
NPIPL3
| nuclear pore complex interacting protein-like 3 | 1.64 | 1.52E-04 |
8019280 |
PCYT2
| phosphate cytidylyltransferase 2; ethanolamine | -1.54 | 7.55E-04 |
EPCa | ||||
7902400 |
SNORD45B
| small nucleolar RNA; C/D box 45B | -4.71 | 2.29E-04 |
7980861 |
CATSPERB
| cation channel; sperm-associated; beta | 1.73 | 5.81E-04 |
8057004 |
PDE11A
| phosphodiesterase 11A | 2.59 | 6.68E-04 |
8004325 |
EIF5A
| eukaryotic translation initiation factor 5A | -1.50 | 7.72E-04 |
7969050 |
CYSLTR2
| cysteinyl leukotriene receptor 2 | 2.50 | 9.01E-04 |
Pathway | - log10 (P) |
---|---|
All (OB/OW versus lean) | |
Glycerolipid metabolism | 2.20 |
Lysine degradation | 2.08 |
Leptin signaling in obesity | 1.78 |
BPH (OB/OW versus lean)
| |
ERK5 signaling | 2.31 |
β-alanine metabolism | 1.83 |
AMPK signaling | 1.81 |
Parkinson's signaling | 1.77 |
C21-steroid hormone metabolism | 1.77 |
EPCa (OB/OW versus lean)
| |
Histidine metabolism | 1.91 |
Eicosanoid signaling | 1.77 |
Linoleic acid metabolism | 1.73 |
All (EPCa versus BPH)
| |
Antigen presentation pathway | 3.11 |
Aminosugars metabolism | 2.09 |
B cell development | 1.81 |
OX40 signaling pathway | 1.77 |
ERK5 signaling | 1.59 |
p53 signaling | 1.55 |
Lean (EPCa versus BPH)
| |
T helper cell differentiation | 2.24 |
Galactose metabolism | 1.68 |
Hereditary breast cancer signaling | 1.62 |
Role of osteoblasts, osteoclasts and condrocytes in rheumatoid arthritis | 1.51 |
OB/OW (EPCa versus BPH)
| |
Synaptic long term depression | 2.89 |
Urea cycle and metabolism of aminogroups | 2.10 |
p53 signaling | 2.10 |
Amyothropic lateral sclerosis signaling | 2.02 |
Aminosugars metabolism | 1.89 |
Extrinsic prothrombin activation pathway | 1.73 |
p70S6K signaling | 1.68 |
Cardiac β-adrenegic signaling | 1.51 |
Defining the PP adipose tissue gene expression signature in prostate cancer patients
Probe set | Gene Name | Gene Description | Fold Change |
P
|
---|---|---|---|---|
Extra Prostatic Cancer versus Benign Prostatic Hyperplasia | ||||
All subjects | ||||
7952313
|
MIRLET7A2
|
microRNA let-7a-2
|
2.00
|
4.26E-06
|
7957540
|
MRPL42
|
mitochondrial ribosomal protein L42
|
1.63
|
5.49E-06
|
7980891
|
TC2N
|
tandem C2 domains; nuclear
|
2.25
|
8.26E-06
|
7915468
|
CCDC23
|
coiled-coil domain containing 23
|
1.70
|
1.93E-05
|
8002020 |
TPPP3
| tubulin polymerization-promoting protein family member 3 | 1.66 | 8.62E-05 |
7980861 |
CATSPERB
| cation channel; sperm-associated; beta | 1.84 | 1.48E-04 |
7964927 |
TSPAN8
| tetraspanin 8 | 1.91 | 2.31E-04 |
8168463 |
FGF16
| fibroblast growth factor 16 | 1.67 | 2.93E-04 |
Lean subjects | ||||
7952313 |
MIRLET7A2
| microRNA let-7a-2 | 2.12 | 9.04E-05 |
8024485 |
GADD45B
| growth arrest and DNA-damage-inducible; beta | -1.56 | 9.47E-04 |
7957540 |
MRPL42
| mitochondrial ribosomal protein L42 | 1.60 | 9.86E-04 |
OB/OW subjects | ||||
7980861
|
CATSPERB
|
cation channel; sperm-associated; beta
|
2.50
|
1.91E-06
|
7980891
|
TC2N
|
tandem C2 domains; nuclear
|
2.98
|
4.31E-06
|
7908488
|
CFHR1
|
complement factor H-related 1
|
1.88
|
3.16E-05
|
8078529
|
STAC
|
SH3 and cysteine rich domain
|
1.50
|
5.79E-05
|
7902400 |
SNORD45B
| small nucleolar RNA; C/D box 45B | -5.18 | 1.9E-04 |
7908459 |
CFH
| complement factor H | 1.70 | 3.0E-04 |
7952313 |
MIRLET7A2
| microRNA let-7a-2 | 1.88 | 4.0E-04 |
7957540 |
MRPL42
| mitochondrial ribosomal protein L42 | 1.65 | 4.0E-04 |
7958019 |
DRAM1
| DNA-damage regulated autophagy modulator 1 | 1.55 | 5.3E-04 |
7969640 |
CLDN10
| claudin 10 | 1.59 | 6.3E-04 |
8076586 |
SCUBE1
| signal peptide; CUB domain; EGF-like 1 | -1.73 | 7.2E-04 |
8027862 |
FFAR2
| free fatty acid receptor 2 | -1.58 | 9.2E-04 |
Organ Confined Prostate Cancer versus Benign Prostatic Hyperplasia
| ||||
All subjects | ||||
7919349
|
RNU1-1
|
RNA; U1 small nuclear 1
|
-2.18
|
1.50E-07
|
7919576
|
RNU1-1
|
RNA; U1 small nuclear 1
|
-1.96
|
2.47E-07
|
7952313 |
MIRLET7A2
| microRNA let-7a-2 | 1.79 | 6.21E-05 |
7980891 |
TC2N
| tandem C2 domains; nuclear | 1.98 | 6.26E-05 |
7978568 |
RNU1-1
| RNA; U1 small nuclear 1 | -1.65 | 9.43E-05 |
7981964 |
SNORD116-8
| small nucleolar RNA; C/D box 116-8 | -1.91 | 1.07E-04 |
8004184 |
XAF1
| XIAP associated factor 1 | -1.64 | 8.67E-04 |
Lean subjects | ||||
8001067
|
HERC2P4
|
hect domain and RLD 2 pseudogene 4
|
-1.77
|
1.22E-06
|
7997239
|
PDXDC2
|
pyridoxal-dependent decarboxylase domain containing 2
|
-2.12
|
2.69E-06
|
8000692
|
BOLA2
|
bolA homolog 2 (E, coli)
|
-1.52
|
3.41E-06
|
8000651
|
SMG1
|
SMG1 homolog; phosphatidylinositol 3-kinase-related kinase (C. elegans)
|
-1.61
|
3.76E-06
|
8019655
|
TBC1D3B
|
TBC1 domain family; member 3B
|
-1.56
|
1.26E-05
|
7919349
|
RNU1-1
|
RNA; U1 small nuclear 1
|
-2.30
|
2.27E-05
|
7927513
|
FAM21C
|
family with sequence similarity 21; member C
|
-1.66
|
2.31E-05
|
7993359
|
NPIP
|
nuclear pore complex interacting protein
|
-1.53
|
4.30E-05
|
8014633
|
TBC1D3
|
TBC1 domain family; member 3
|
-1.55
|
5.53E-05
|
8014437
|
TBC1D3G
|
TBC1 domain family; member 3G
|
-1.51
|
6.27E-05
|
7994026
|
NPIPL3
|
nuclear pore complex interacting protein-like 3
|
-1.69
|
9.02E-05
|
OB/OW subjects | ||||
7969640
|
CLDN10
|
claudin 10
|
2.01
|
7.04E-06
|
7980891 |
TC2N
| tandem C2 domains; nuclear | 2.31 | 1.10E-04 |
7952313 |
MIRLET7A2
| microRNA let-7a-2 | 2.06 | 1.74E-04 |
8021101 |
HAUS1
| HAUS augmin-like complex; subunit 1 | 1.61 | 3.10E-04 |
8165698 |
MIR1977
| microRNA 1977 | -3.03 | 3.46E-04 |
8060854 |
PLCB1
| phospholipase C; beta 1 (phosphoinositide-specific) | 1.53 | 5.95E-04 |
Extra Prostatic Cancer versus Organ Confined Prostate Cancer
| ||||
All subjects | ||||
7919349 |
RNU1-1
| RNA; U1 small nuclear 1 | 1.71 | 1.86E-05 |
7981964 |
SNORD116-8
| small nucleolar RNA; C/D box 116-8 | 1.88 | 1.85E-04 |
7940287 |
MS4A1
| membrane-spanning 4-domains; subfamily A; member 1 | -2.15 | 2.74E-04 |
Lean subjects | ||||
8001067
|
HERC2P4
|
hect domain and RLD 2 pseudogene 4
|
2.06
|
5.14E-08
|
8000692
|
BOLA2
|
bolA homolog 2 (E, coli)
|
1.52
|
3.37E-06
|
7997239
|
PDXDC2
|
pyridoxal-dependent decarboxylase domain containing 2
|
2.00
|
6.36E-06
|
8000651
|
SMG1
|
SMG1 homolog; phosphatidylinositol 3-kinase-related kinase (C. elegans)
|
1.57
|
6.75E-06
|
7927513
|
FAM21C
|
family with sequence similarity 21; member C
|
1.74
|
8.44E-06
|
7993359
|
NPIP
|
nuclear pore complex interacting protein
|
1.51
|
5.38E-05
|
7994026
|
NPIPL3
|
nuclear pore complex interacting protein-like 3
|
1.73
|
5.61E-05
|
8119595
|
RPL7L1
|
ribosomal protein L7-like 1
|
1.63
|
5.71E-05
|
7990943
|
GOLGA6L10
|
golgin A6 family-like 10
|
1.55
|
6.08E-05
|
7919349 |
RNU1-1
| RNA; U1 small nuclear 1 | 1.93 | 2.25E-04 |
OB/OW subjects | ||||
7980861 |
CATSPERB
| cation channel; sperm-associated; beta | 1.78 | 2.92E-04 |
7982006 |
SNORD116-29
| small nucleolar RNA; C/D box 116-29 | 1.77 | 5.84E-04 |