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Erschienen in:
01.04.2010 | Original Article
Optimization of the non-invasive 13C-sucrose breath test in a rat model of methotrexate-induced mucositis
Erschienen in: Cancer Chemotherapy and Pharmacology | Ausgabe 5/2010
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Purpose
In order to determine the sensitivity and specificity of the test and to optimize experimental conditions utilizing the SBT in a rat model of chemotherapy-induced small intestinal damage.
Methods
Initially, a 13C-sucrose dose-response study was performed in rats to determine an optimal sucrose concentration for the SBT; then applied to assess chemotherapy-induced intestinal damage. A further study was conducted to establish a SBT time-course of methotrexate-induced small intestinal damage and repair. Animals were killed at 96 or 144 h.
Results
A sucrose concentration of 0.25 g/ml was optimal (20% CV) for reproducibility and detection of intestinal damage. Maximal damage occurred at 72 h, small intestinal repair was initiated by 96 h and continued at 144 h post-MTX, as determined by the SBT and confirmed by biochemical analyses. Levels of sensitivity and specificity for the SBT were 98 and 94%, respectively.
Conclusions
The SBT is a reliable non-invasive marker of small intestinal health and damage with a high degree of sensitivity and specificity.