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04.11.2016 | Article

Peptide serum markers in islet autoantibody-positive children

verfasst von: Christine von Toerne, Michael Laimighofer, Peter Achenbach, Andreas Beyerlein, Tonia de las Heras Gala, Jan Krumsiek, Fabian J. Theis, Anette G. Ziegler, Stefanie M. Hauck

Erschienen in: Diabetologia | Ausgabe 2/2017

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Abstract

Aims/hypothesis

We sought to identify minimal sets of serum peptide signatures as markers for islet autoimmunity and predictors of progression rates to clinical type 1 diabetes in a case–control study.

Methods

A double cross-validation approach was applied to first prioritise peptides from a shotgun proteomic approach in 45 islet autoantibody-positive and -negative children from the BABYDIAB/BABYDIET birth cohorts. Targeted proteomics for 82 discriminating peptides were then applied to samples from another 140 children from these cohorts.

Results

A total of 41 peptides (26 proteins) enriched for the functional category lipid metabolism were significantly different between islet autoantibody-positive and autoantibody-negative children. Two peptides (from apolipoprotein M and apolipoprotein C-IV) were sufficient to discriminate autoantibody-positive from autoantibody-negative children. Hepatocyte growth factor activator, complement factor H, ceruloplasmin and age predicted progression time to type 1 diabetes with a significant improvement compared with age alone.

Conclusion/interpretation

Distinct peptide signatures indicate islet autoimmunity prior to the clinical manifestation of type 1 diabetes and enable refined staging of the presymptomatic disease period.

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Titel: Peptide serum markers in islet autoantibody-positive children
verfasst von: Christine von Toerne
Michael Laimighofer
Peter Achenbach
Andreas Beyerlein
Tonia de las Heras Gala
Jan Krumsiek
Fabian J. Theis
Anette G. Ziegler
Stefanie M. Hauck
Publikationsdatum: 04.11.2016
Verlag: Springer Berlin Heidelberg
Erschienen in: Diabetologia / Ausgabe 2/2017
Print ISSN: 0012-186X
Elektronische ISSN: 1432-0428
DOI: https://doi.org/10.1007/s00125-016-4150-x

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