The authors declare that they have no competing interests.
DCH: Dr. Hedgpeth is a periodontist who recruited patients, collected periodontal tissues after surgeries and provided clinical data. XZ: Xiaoming is a research specialist who carried out RNA isolation and gene expression analysis using real-time PCR. JJ: Dr. Jin is a staff scientist who performed gene expression analysis using real-time PCR and data analysis. RSL: Dr. Leite is a periodontist who recruited patients, collected periodontal tissues after surgeries, provided clinical data, and contributed to manuscript preparation. JWK: Dr. Krayer is a periodontist who contributed to manuscript preparation. YH: Dr. Huang is a senior investigator overseeing the project including obtaining ethical approval, experimental design, data analysis and manuscript preparation. All authors read and approved the final manuscript.
Patients with type 2 diabetes mellitus (T2DM) have increased severity of periodontitis. Toll-like receptor (TLR)4, its co-receptors CD14 and MD-2, and adaptor MyD88 play pivotal roles in lipopolysaccharide (LPS)-triggered tissue inflammation and periodontitis. This study investigated the effects of T2DM and periodontitis on TLR4, CD14, MD-2 and MyD88 mRNA expression in surgically removed periodontal tissues.
Periodontal tissue specimens were collected from 14 patients without periodontitis and T2DM (Group 1), 15 patients with periodontitis alone (Group 2), and 7 patients with both periodontitis and T2DM (Group 3). The mRNA of TLR4, CD14, MD-2 and MyD88 was quantified using real-time PCR and compared between the groups.
Statistical analysis showed that periodontal expression of CD14 mRNA was significantly reduced across Groups 1, 2 and 3 (p = 0.02) whereas the mRNA expression of TLR4, MD-2 and MyD88 was not significantly different among the groups. Furthermore, when patients in Groups 1 and 2 were combined (n = 22), the CD14 mRNA expression was significantly lower than that in patients of Group 1 (p = 0.04).
CD14 mRNA expression was downregulated across patients with neither periodontitis nor T2DM, patients with periodontitis alone and patients with both diseases, suggesting that CD14 mRNA expression is associated with a favorable host response or subjected to a negative feedback regulation.
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- Periodontal CD14 mRNA expression is downregulated in patients with chronic periodontitis and type 2 diabetes
Dustin C. Hedgpeth
Renata S. Leite
Joe W. Krayer
- BioMed Central
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