Erschienen in:
01.09.2014 | Original Article
Pharmacokinetics of Lopinavir Determined with an ELISA Test in Youths with Perinatally Acquired HIV
verfasst von:
Roberta Prinapori, Raffaella Rosso, Antonio Di Biagio, Franca Miletich, Elisa Furfaro, Lucia Taramasso, Francesca Ginocchio, Vania Giacomet, Loredana Nulvesu, Maria Pia Sormani, Irene Schiavetti, Alessio Signori, Laura De Hoffer, Claudio Viscoli
Erschienen in:
Indian Journal of Pediatrics
|
Ausgabe 9/2014
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Abstract
Objective
To investigate the plasma levels of lopinavir by enzyme-linked immunosorbent assay (ELISA) in a cohort of patients who were vertically infected with human immunodeficiency virus 1 (HIV).
Methods
Plasma levels of lopinavir (Cmin) were determined by ELISA test in patients treated with lopinavir/ritonavir-based combined antiretroviral therapy who had achieved virological response after 4 wk of therapy. Reference lopinavir concentrations were Cmin 1–8 μg/mL. Correlation between lopinavir plasma concentration and continuous variables was evaluated by mean of Pearson correlation coefficient. Differences in lopinavir (LPV) concentration for binary categorical variables were assessed by Mann-Whitney test, while for variables with more than two categories Kruskal-Wallis test was used.
Results
Thirty-four patients were enroled; median age was 133 mo (15–265). The median lopinavir dose tested was 383.5 mg/kg (IQR: 266.6–400 mg/kg), with a median plasma concentration of 8.8 μg/mL (IQR: 5–14 μg/mL). Lopinavir Cmin was <1 μg/mL in only one sample (2.9 %), while 14 samples had Cmin between 1 and 8 μg/mL (41.2 %) and 19 (55.9 %) > 8 μg/mL. No significant correlations were found between plasma concentrations of lopinavir and the continuous variables considered in the study. A negative but, not completely significant, correlation was found between plasma drug concentration and body mass index (r = −0.29; p = 0.09).
Conclusions
The use of a simple and relatively cost-effective methodology might render therapeutic drug monitoring (TDM) appeal in the daily clinical practice.