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Journal of Cancer Research and Clinical Oncology

Erschienen in:

08.03.2017 | Original Article – Cancer Research

Phosphorylase kinase β affects colorectal cancer cell growth and represents a novel prognostic biomarker

verfasst von: Guanghui Wang, Wenbin Shen, Chen-ying Liu, Yun Liu, Tingyu Wu, Ximao Cui, Tong Yu, Yilian Zhu, Jinglue Song, Peng Du, Long Cui

Erschienen in: Journal of Cancer Research and Clinical Oncology | Ausgabe 6/2017

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Abstract

Purpose

To study the expression and intracellular localization of phosphorylase kinase β (PHKβ) protein in colorectal cancers (CRCs), analyze its correlation with clinicopathological features and prognosis, and study the biological roles and mechanism-of-action of PHKβ in CRC cell lines.

Methods

Quantitative polymerase chain reaction (qPCR) and western blot assays were performed to compare the expressions of PHKβ mRNA and protein in CRC tissues and matched normal mucosa. Tissue microarrays and immunohistochemical staining were performed to detect the expression and intracellular location of PHKβ protein and analyze its correlation with the clinicopathological characteristics and prognosis in CRC patients. Proliferation, cell cycle, wound healing, and xenograft models were used to elucidate the potential role of PHKβ in vitro and in vivo.

Results

PHKβ mRNA and protein were found to be overexpressed in CRC tissue compared to the levels in normal mucosa. Positive expression of PHKβ was significantly correlated with TNM stage and distal metastasis, and elevated expression of PHKβ was an independent prognostic factor in patients with CRC. PHKβ knockdown impaired proliferation of CRC in vitro and in vivo and induced cell cycle arrest.

Conclusions

PHKβ affects CRC cell growth and represents a novel prognostic biomarker.

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Titel: Phosphorylase kinase β affects colorectal cancer cell growth and represents a novel prognostic biomarker
verfasst von: Guanghui Wang
Wenbin Shen
Chen-ying Liu
Yun Liu
Tingyu Wu
Ximao Cui
Tong Yu
Yilian Zhu
Jinglue Song
Peng Du
Long Cui
Publikationsdatum: 08.03.2017
Verlag: Springer Berlin Heidelberg
Erschienen in: Journal of Cancer Research and Clinical Oncology / Ausgabe 6/2017
Print ISSN: 0171-5216
Elektronische ISSN: 1432-1335
DOI: https://doi.org/10.1007/s00432-017-2362-1

Springer Medizin

Phosphorylase kinase β affects colorectal cancer cell growth and represents a novel prognostic biomarker