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07.04.2021 | Original Article

Photobiomodulation effect of red LED (630 nm) on the free radical levels produced by pulp cells under stress conditions

verfasst von: Jéssica Fernanda Sena Bonvicini, Fernanda Gonçalves Basso, Carlos Alberto de Souza Costa, Carlos José Soares, Ana Paula Turrioni

Erschienen in: Lasers in Medical Science | Ausgabe 1/2022

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Abstract

The aim of this study was to assess the ability of red light emitting diodes (LED) to modulate oxidative stress in human dental pulp fibroblasts (HDPFs) when different irradiation parameters are employed. Cells from primary teeth were seeded (100,000 cells/well) in 24-well plates in culture medium (DMEM). At 24 h after incubation, the culture medium was replaced with DMEM containing 10 μg/mL lipopolysaccharide (LPS). Thereafter, the cells were irradiated (LED 630 nm, 0.04 W/cm² and 0.08 W/cm²) at 0 J/cm² (control group), 4 J/cm², 15 J/cm², and 30 J/cm²; and their viability (MTT assay), number (Trypan Blue), synthesis of nitric oxide (NO) (Griess reagent), and reactive oxygen species (ROS) (fluorescence probe, DCFH-DA) were assessed. The Kruskal-Wallis and Mann-Whitney statistical tests using Bonferroni correction were employed (significance level of 5%). Compared to that in control fibroblasts, increased viability was observed in HDPFs exposed to LPS and irradiated with 15 J/cm² and 30 J/cm² at 0.04 W/cm² and 4 J/cm² and 15 J/cm² at 0.08 W/cm² (p < 0.05). Exposure to 4 J/cm² at 0.04 W/cm² and 15 J/cm² and 30 J/cm² at 0.08 W/cm² modulated the oxidative stress in cells relative to that observed in non-irradiated LPS-treated pulp cells (p < 0.05). It was concluded that the irradiation strategies of using red LED with radiant exposures of 15 J/cm² and 30 J/cm² at 0.04 W/cm² and 15 J/cm² at 0.08 W/cm² were the best parameters to decrease NO and ROS concentration and to stimulate viability of HDPFs exposed to LPS challenge.

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Titel: Photobiomodulation effect of red LED (630 nm) on the free radical levels produced by pulp cells under stress conditions
verfasst von: Jéssica Fernanda Sena Bonvicini
Fernanda Gonçalves Basso
Carlos Alberto de Souza Costa
Carlos José Soares
Ana Paula Turrioni
Publikationsdatum: 07.04.2021
Verlag: Springer London
Erschienen in: Lasers in Medical Science / Ausgabe 1/2022
Print ISSN: 0268-8921
Elektronische ISSN: 1435-604X
DOI: https://doi.org/10.1007/s10103-021-03309-x

Springer Medizin

Abstract

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