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Strahlentherapie und Onkologie

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01.12.2012 | Original article

Plasminogen activator inhibitor-1 (PAI-1) expression in relation to hypoxia and oncoproteins in clinical cervical tumors

verfasst von: P.C. Lara, M. Lloret, A. Valenciano, B. Clavo, B. Pinar, A. Rey, L. A. Henríquez-Hernández

Erschienen in: Strahlentherapie und Onkologie | Ausgabe 12/2012

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Abstract

Ziel

Ziel war es, die Rolle von PAI-1 und seinen Zusammenhang mit Hypoxie und der Expression von p53, Ku70/80 und Cyclin D1 bei Zervixkarzinompatientinnen zu erforschen.

Material und Methoden

PAI-1, Cyclin D1 und p53 sowie die Tumoroxygenierung wurden bei 43 aufeinanderfolgenden Patientinnen mit lokalisiertem Zervixkarzinom bestimmt. Die Expression der Onkoproteine wurde mittels Immunhistochemie identifiziert. Die Tumoroxygenierung wurde mithilfe eines polarographischen Probensystems “pO₂ Histography” gemessen.

Ergebnisse

Die PAI-Expression wurde in 32,6% als negativ und in 18,6% der Fälle als überexprimiert angesehen. Cyclin D1 zeigte eine mittlere Expression von 5,0 (Spanne: 0–70). Wir beobachteten einen positiven Zusammenhang zwischen PAI-Expression und verändertem p53 (p = 0,049) und Cyclin D1 (p = 0,02). Ein antiproportionales Verhältnis wurde für die PAI- und Ku70/80-Expression festgestellt (p = 0,042). Die Cyclin-D1-Färbung nahm mit dem Tumorvolumen zu (r = 0,314, p = 0,009). Es konnte kein signifikanter Zusammenhang zwischen PAI und Hypoxämie oder anderen klinisch-pathologischen Parametern beobachtet werden.

Schlussfolgerung

Den vorliegenden Ergebnisse zufolge scheint die PAI-2-Überexpression – neben der Downregulierung der DNA-Reparatur mittels nichthomologem Endjoining (niedrige Ku70/80-Expression) in Kombination mit einer erhöhten p53- und Cyclin-D1-Expression – am Tumorverhalten des Zervixkarzinoms beteiligt zu sein.

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Titel: Plasminogen activator inhibitor-1 (PAI-1) expression in relation to hypoxia and oncoproteins in clinical cervical tumors
verfasst von: P.C. Lara
M. Lloret
A. Valenciano
B. Clavo
B. Pinar
A. Rey
L. A. Henríquez-Hernández
Publikationsdatum: 01.12.2012
Verlag: Springer-Verlag
Erschienen in: Strahlentherapie und Onkologie / Ausgabe 12/2012
Print ISSN: 0179-7158
Elektronische ISSN: 1439-099X
DOI: https://doi.org/10.1007/s00066-012-0216-y

Springer Medizin

Plasminogen activator inhibitor-1 (PAI-1) expression in relation to hypoxia and oncoproteins in clinical cervical tumors

Abstract