nach oben

European Journal of Clinical Microbiology & Infectious Diseases

Erschienen in:

16.10.2020 | Brief Report

Pooling of samples to optimize SARS-CoV-2 diagnosis by RT-qPCR: comparative analysis of two protocols

verfasst von: Fabiana Volpato, Daiana Lima-Morales, Priscila Lamb Wink, Julia Willig, Fernanda de-Paris, Patricia Ashton-Prolla, Afonso Luís Barth

Erschienen in: European Journal of Clinical Microbiology & Infectious Diseases | Ausgabe 4/2021

Einloggen, um Zugang zu erhalten

Abstract

This study has aimed to evaluate the use pool of samples as a strategy to optimize the diagnostic of SARS-CoV-2 by RT-qPCR. A total of 220 naso/orofaryngeal swab samples were collected and tested using two different protocols of sample pooling. Results from protocol A were identical with the individual results. However, for results from protocol B, reduced agreement (91%) was observed in relation to individual testing. Inconsistencies observed were related to RT-qPCR results with higher cycle thresholds. These results suggest that pooling of samples before RNA extraction is preferable in terms of diagnostic for SARS-CoV-2.

Sun J, He WT, Wang L, Lai A, Ji X, Zhai X, Li G, Suchard MA, Tian J, Zhou J, Veit M, Su S (2020) COVID-19: epidemiology, evolution, and cross-disciplinary perspectives. Trends Mol Med 26:483–495. https://doi.org/10.1016/j.molmed.2020.02.008CrossRefPubMedPubMedCentral

Tang YW, Schmitz JE, Persing DH, Stratton CW (2020) Laboratory diagnosis of COVID-19: current issues and challenges. J Clin Microbiol 58:e00512–e00520. https://doi.org/10.1128/JCM.00512-20CrossRefPubMedPubMedCentral

Lu X, Whitaker B, Sakthivel SKK, Kamili S, Rose LE, Lowe L, Mohareb E, Elassal EM, Al-sanouri T, Haddadin A, Erdman DD (2014) Real-time reverse transcription-PCR assay panel for Middle East respiratory syndrome coronavirus. J Clin Microbiol 52(1):67–75. https://doi.org/10.1128/JCM.02533-13CrossRefPubMedPubMedCentral

Shen M, Zhou Y, Ye J, Al-maskri AAA, Kang Y, Zeng S, Cai S (2020) Recent advances and perspectives of nucleic acid detection for coronavirus. J Pharm Anal 10:97–101. https://doi.org/10.1016/j.jpha.2020.02.010CrossRefPubMedPubMedCentral

Nguyen T, Bang DD, Wolff A (2020) 2019 novel coronavirus disease (COVID-19): paving the road for rapid detection and point-of-care diagnostics. Micromachines 11. https://doi.org/10.3390/mi11030306

Lohse S, Pfuhl T, Berkó-Göttel B, Rissland J, Geiβler T, Gärtner B (2020) Pooling of samples for testing for SARS-CoV-2 in asymptomatic people. Lancet Infect Dis. https://doi.org/10.1016/S1473-3099(20)30362-5

Buckingham L (2019) Chapter 6: nucleic acid amplification. In Buckingham L. Molecular diagnostics: Fundamentals, methods, and clinical applications, 3rd edn. F.A. Davis Company, Philadelphia, p 142–178

Titel: Pooling of samples to optimize SARS-CoV-2 diagnosis by RT-qPCR: comparative analysis of two protocols
verfasst von: Fabiana Volpato
Daiana Lima-Morales
Priscila Lamb Wink
Julia Willig
Fernanda de-Paris
Patricia Ashton-Prolla
Afonso Luís Barth
Publikationsdatum: 16.10.2020
Verlag: Springer Berlin Heidelberg
Erschienen in: European Journal of Clinical Microbiology & Infectious Diseases / Ausgabe 4/2021
Print ISSN: 0934-9723
Elektronische ISSN: 1435-4373
DOI: https://doi.org/10.1007/s10096-020-04071-8

Leitlinien kompakt für die Innere Medizin

Mit medbee Pocketcards sicher entscheiden.

^{Seit 2022 gehört die medbee GmbH zum Springer Medizin Verlag}

Kostenlos registrieren

Neu im Fachgebiet Innere Medizin

25.04.2024 | Hypotonie | Nachrichten

Update Innere Medizin

Bestellen Sie unseren Fach-Newsletter und bleiben Sie gut informiert.

Newsletter bestellen

Springer Medizin

Pooling of samples to optimize SARS-CoV-2 diagnosis by RT-qPCR: comparative analysis of two protocols

Abstract

Leitlinien kompakt für die Innere Medizin

Neu im Fachgebiet Innere Medizin

Niedriger diastolischer Blutdruck erhöht Risiko für schwere kardiovaskuläre Komplikationen

Therapiestart mit Blutdrucksenkern erhöht Frakturrisiko

Viel Bewegung in der Parkinsonforschung

Adjuvante Immuntherapie verlängert Leben bei RCC

Update Innere Medizin

Springer Medizin

Abstract

Bitte loggen Sie sich ein, um Zugang zu diesem Inhalt zu erhalten

Weitere Artikel der Ausgabe 4/2021

Multi-year prevalence and macrolide resistance of Mycoplasma genitalium in clinical samples from a southern Italian hospital

Bloodstream infection due to Herbaspirillum sp.: case series and review of literature

Concomitant emphysema might increase the false-negative rate of urinary antigen tests in patients with pneumococcal pneumonia: results from a retrospective study

Epidemiological of cat scratch disease among inpatients in the Spanish health system (1997–2015)

The prevalence and risks of major comorbidities among inpatients with pulmonary tuberculosis in China from a gender and age perspective: a large-scale multicenter observational study

Predicting in-hospital mortality for sepsis: a comparison between qSOFA and modified qSOFA in a 2-year single-centre retrospective analysis

Leitlinien kompakt für die Innere Medizin

Neu im Fachgebiet Innere Medizin

Niedriger diastolischer Blutdruck erhöht Risiko für schwere kardiovaskuläre Komplikationen

Therapiestart mit Blutdrucksenkern erhöht Frakturrisiko

Viel Bewegung in der Parkinsonforschung

Adjuvante Immuntherapie verlängert Leben bei RCC

Update Innere Medizin