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European Journal of Clinical Microbiology & Infectious Diseases

Erschienen in:

16.09.2021 | Brief Report

Possible misinterpretation of penicillin susceptibility in Staphylococcus aureus blood isolate due to in vitro loss of the blaZ gene

verfasst von: Helle Brander Eriksen, Andreas Petersen, Michael Pedersen, Søren Overballe-Petersen, Anders Rhod Larsen, Barbara Juliane Holzknecht

Erschienen in: European Journal of Clinical Microbiology & Infectious Diseases | Ausgabe 1/2022

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Abstract

We describe a case of recurrent catheter-related blood stream infections (BSI) with Staphylococcus aureus, in which the first isolate tested susceptible to penicillin, while subsequent isolates were resistant. Phenotypic susceptibility correlated with the absence/presence of the blaZ gene. The in vitro stability of penicillin resistance was investigated by subculturing single colonies. In two out of five colonies, phenotypical resistance was lost after a single subculture, which correlated with loss of the blaZ gene. This in vitro phenomenon probably resulted in a very major error in the microbiology report of the first BSI, where penicillin had been recommended as treatment.

DANMAP (2019) Use of antimicrobial agents and occurrence of antimicrobial resistance in bacteria from food animals, food and humans in Denmark. ISSN 1600-2032. www.danmap.org

HagstrandAldman M, Skovby A, Påhlman LI (2017) Penicillin-susceptible Staphylococcus aureus : susceptibility testing, resistance rates and outcome of infection. Infect Dis (Auckl) 49:454–60. https://doi.org/10.1080/23744235.2017.1280617CrossRef

Richter SS, Doern GV, Heilmann KP, Miner S, Tendolkar S, Riahi F et al (2016) Detection and prevalence of penicillin-susceptible Staphylococcus aureus in the United States in 2013. J Clin Microbiol 54:812–814. https://doi.org/10.1128/JCM.03109-15CrossRefPubMedPubMedCentral

Foster TJ (2017) Antibiotic resistance in Staphylococcus aureus. Current status and future prospects. FEMS Microbiol Rev 41:430–49. https://doi.org/10.1093/femsre/fux007CrossRefPubMed

El Feghaly RE, Stamm JE, Fritz SA, Burnham CAD (2012) Presence of the blaZbeta-lactamase gene in isolates of Staphylococcus aureus that appear penicillin susceptible by conventional phenotypic methods. Diagn Microbiol Infect Dis 74:388–393. https://doi.org/10.1016/j.diagmicrobio.2012.07.013CrossRefPubMed

Larsen AR, Stegger M, Sørum M (2008) Spa typing directly from a mecA, spa and pvl multiplex PCR assay-a cost-effective improvement for methicillin-resistant Staphylococcus aureus surveillance. Clin Microbiol Infect 14:611–614. https://doi.org/10.1111/j.1469-0691.2008.01995.xCrossRefPubMed

Kaase M, Lenga S, Friedrich S, Szabados F, Sakinc T, Kleine B et al (2008) Comparison of phenotypic methods for penicillinase detection in Staphylococcus aureus. Clin Microbiol Infect 14:614–616. https://doi.org/10.1111/j.1469-0691.2008.01997.xCrossRefPubMed

Hammerum AM, Porsbo LJ, Hansen F, Roer L, Kaya H, Henius A et al (2020) Surveillance of OXA-244-producing Escherichia coli and epidemiologic investigation of cases, Denmark, January 2016 to August 2019. Eurosurveillance 25:1–9. https://doi.org/10.2807/1560-7917.ES.2020.25.18.1900742CrossRef

Sahl JW, Lemmer D, Travis J, Schupp JM, Gillece JD, Aziz M et al (2016) NASP: an accurate, rapid method for the identification of SNPs in WGS datasets that supports flexible input and output formats. Microb genomics 2:e000074. https://doi.org/10.1099/mgen.0.000074CrossRef

10.

Leopold SR, Goering RV, Witten A, Harmsen D, Mellmann A (2014) Bacterial whole-genome sequencing revisited: portable, scalable, and standardized analysis for typing and detection of virulence and antibiotic resistance genes. J Clin Microbiol 52:2365–2370. https://doi.org/10.1128/JCM.00262-14CrossRefPubMedPubMedCentral

11.

Sieber RN, Overballe-Petersen S, Kaya H, Larsen AR, Petersen A (2020) Complete genome sequences of methicillin-resistant Staphylococcus aureus strains 110900 and 128254, two representatives of the CRISPR-Cas-carrying sequence type 630/spa type t4549 lineage. Microbiol Resour Announc 9:1–3CrossRef

12.

Barber M (1949) The incidence of penicillin-sensitive variant colonies in penicillinase-producing strains of Staphylococcus pyogenes. J Gen Microbiol 3(2):274–281. https://doi.org/10.1099/00221287-3-2-274

13.

Olsen JE, Christensen H, Aarestrup FM (2006) Diversity and evolution of blaZ from Staphylococcus aureus and coagulase-negative staphylococci. J Antimicrob Chemother 57:450–460. https://doi.org/10.1093/jac/dki492CrossRefPubMed

14.

Mama OM, Aspiroz C, Lozano C, Ruiz-Ripa L, Azcona JM, Seral C, Cercenado E, López-Cerero L, Palacian P, Belles-Belles A, Berdonces P, Siller M, Aguirre-Quiñonero A, Zarazaga M, Torres C, Spanish Study Group of Clinical S. aureus CC398 (2021) Penicillin susceptibility among invasive MSSA infections: a multicentre study in 16 Spanish hospitals. J Antimicrob Chemother 7:dkab208. https://doi.org/10.1093/jac/dkab208

15.

Gargis AS, Yoo BB, Lonsway DR, Anderson K, Campbell D, Ewing TO, Lawsin A, Machado MJ, Yamamoto N, Halpin AL, Lutgring JD, Karlsson M, Rasheed JK, Elkins CA (2020) Difficult-to-detect staphylococcus aureus: mecA-positive isolates associated with oxacillin and cefoxitin false-susceptible results. J Clin Microbiol 58(4):e02038-19. https://doi.org/10.1128/JCM.02038-19

16.

Goering RV, Swartzendruber EA, Obradovich AE, Tickler IA, Tenover FC (2019) Emergence of oxacillin resistance in stealth methicillin-resistant staphylococcus aureus due to mecA sequence instability. Antimicrob Agents Chemother 63(8):e00558-19. https://doi.org/10.1128/AAC.00558-19

17.

Nissen JL, Skov R, Knudsen JD, Østergaard C, Schønheyder HC, Frimodt-MØller N et al (2013) Effectiveness of penicillin, dicloxacillin and cefuroxime for penicillin-susceptible staphylococcus aureus bacteraemia: a retrospective, propensity-score-adjusted case-control and cohort analysis. J Antimicrob Chemother 68:1894–1900. https://doi.org/10.1093/jac/dkt108CrossRefPubMed

18.

Henderson A, Harris P, Hartel G, Paterson D, Turnidge J, Davis JS et al (2019) Benzylpenicillin versus flucloxacillin for penicillin-susceptible Staphylococcus aureus bloodstream infections from a large retrospective cohort study. Int J Antimicrob Agents 54:491–495. https://doi.org/10.1016/j.ijantimicag.2019.05.020CrossRefPubMed

Titel: Possible misinterpretation of penicillin susceptibility in Staphylococcus aureus blood isolate due to in vitro loss of the blaZ gene
verfasst von: Helle Brander Eriksen
Andreas Petersen
Michael Pedersen
Søren Overballe-Petersen
Anders Rhod Larsen
Barbara Juliane Holzknecht
Publikationsdatum: 16.09.2021
Verlag: Springer Berlin Heidelberg
Erschienen in: European Journal of Clinical Microbiology & Infectious Diseases / Ausgabe 1/2022
Print ISSN: 0934-9723
Elektronische ISSN: 1435-4373
DOI: https://doi.org/10.1007/s10096-021-04344-w

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Possible misinterpretation of penicillin susceptibility in Staphylococcus aureus blood isolate due to in vitro loss of the blaZ gene

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