Erschienen in:
10.09.2018 | Translational Research and Biomarkers
Prognostic Impact of Immune-Related Gene Expression in Preoperative Peripheral Blood from Gastric Cancer Patients
verfasst von:
Shuhei Ito, MD, PhD, Takeo Fukagawa, MD, PhD, Miwa Noda, MD, Qingjiang Hu, MD, PhD, Sho Nambara, MD, PhD, Dai Shimizu, MD, PhD, Yosuke Kuroda, MD, PhD, Hidetoshi Eguchi, MD, PhD, Takaaki Masuda, MD, PhD, Tetsuya Sato, PhD, Hitoshi Katai, MD, PhD, Mitsuru Sasako, MD, PhD, Koshi Mimori, MD, PhD
Erschienen in:
Annals of Surgical Oncology
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Ausgabe 12/2018
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Abstract
Background
Anti-PD-1 therapy has shown a promising clinical outcome in gastric cancer (GC). We evaluated the clinical significance of systemic immune-related gene expression in GC patients who underwent surgery.
Methods
The correlation between the preoperative PD-1, PD-L1, and CD8 mRNA levels in peripheral blood (PB) and clinicopathological factors, including survival, in 372 GC patients was evaluated using quantitative RT-PCR. PD-1- and PD-L1-expressing cells were identified by flow cytometric analysis.
Results
The PD-1, PD-L1, and CD8 mRNA levels in GC patients were significantly higher than those in normal controls, respectively (all P < 0.0001). The levels of each gene were positively correlated with those of the other two genes (all P < 0.0001). GC patients with low PD-1, high PD-L1, and low CD8 mRNA levels had significantly poorer overall survival (OS) than those with high PD-1, low PD-L1, and high CD8 mRNA levels, respectively (P < 0.01, P < 0.05, and P < 0.05, respectively). Multivariate analysis showed that low PD-1 and high PD-L1 mRNA levels were independent poor prognostic factors for OS (PD-1: HR 2.38, 95% CI 1.27–4.78, P < 0.01; PD-L1: HR 1.81, 95% CI 1.15–2.78, P < 0.05). PD-1 and PD-L1 expression occurred on T cells (> 90%) and T cells or monocytes (> 70%), respectively.
Conclusions
The PD-1, PD-L1, and CD8 mRNA levels in preoperative PB reflected the anti-tumour immune response, and the low PD-1 and high PD-L1 mRNA levels in PB were independent poor prognostic markers in GC patients who underwent surgery.