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Brain Structure and Function

Erschienen in:

01.11.2006 | Original Article

Promoter of the heat shock testis-specific Hsp70.2/Hst70 gene is active in nervous system during embryonic development of mice

verfasst von: Weronika Rupik, Agata Stawierej, Iwona Stolarczyk, Wieslawa Widłak

Erschienen in: Brain Structure and Function | Ausgabe 6/2006

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Abstract

The Hsp70.2/Hst70 gene is a unique member of the 70 kDa heat shock proteins multigene family whose activity is regulated developmentally; in adult mice and rats its expression is restricted mostly to meiotic and postmeiotic male germ cells. In aim to analyze activity of the Hsp70.2/Hst70 promoter in developing embryos we have constructed transgenic mice expressing EGFP reporter gene under control of the rat Hst70 promoter. The appearance of EGFP fluorescence coincides with series of major developmental events, such as extra-embryonic membranes formation, axial rotation, formation of neural tube and the primordium of central nervous system, formation of differentiated somites, extensive remodeling of the heart, development of fingers and toes, and sensory organs formation. Activity of the Hst70 promoter localizes mostly inside nervous system indicating the role of Hsp70.2/Hst70 gene in development of this system.

Allen JW, Dix DJ, Collins BW, Merrick BA, He C, Selkirk JK, Poorman-Allen P, Dresser ME, Eddy EM (1996) HSP70-2 is part of the synaptonemal complex in mouse and hamster spermatocytes. Chromosoma 104:414–421PubMedCrossRef

Bell AC, West AG, Felsenfeld G (1999) The protein CTCF is required for the enhancer blocking activity of vertebrate insulators. Cell 98:387–396PubMedCrossRef

Beere H (2004) “The stress of dying”: the role of heat shock proteins in the regulation of apoptosis. J Cell Sci 117:2641–2651PubMedCrossRef

Berruti G, Martegani E (2001) MSJ-1, a mouse testis-specific DnaJ protein, is highly expressed in haploid male germ cells and interacts with the testis-specific heat shock protein Hsp70-2. Biol Reprod 65:488–495PubMedCrossRef

Binder RJ, Vatner R, Srivastava P (2004) The heat-shock protein receptors: some answers and more questions. Tissue Antigens 64:442–451PubMedCrossRef

Bonnycastle LLC, Yu Ch-E, Hunt CR, Trask BJ, Clancy KP, Weber JL, Patterson D, Schellenberg GD (1994) Cloning, sequencing, and mapping of the human chromosome 14 heat shock protein gene (HspA2). Genomics 23:85–93PubMedCrossRef

Christians ES, Zhou Q, Renard JP, Benjamin IJ (2003) Heat shock proteins in mammalian development. Cell Dev Biol 14:283–290CrossRef

Dawson HN, Ferreira A, Eyster MV, Ghoshal N, Binder LI, Vitek MP (2001) Inhibition of neuronal maturation in primary hippocampal neurons from tau deficient mice. J Cell Sci 114:1179–1187PubMed

Dix DJ, Allen JW, Collins W, Mori C, Nakamura N, Poorman-Allen P, Goulding EH, Eddy EM (1996) Targeted gene disruption of Hsp70-2 results in failed meiosis, germ cell apoptosis, and male infertility. Proc Natl Acad Sci USA 16:3264–3268CrossRef

Domanico SZ, DeNagel DC, Dahlseid JN, Green JM, Pierce SK (1993) Cloning of the gene encoding peptide-binding protein 74 shows that it is a new member of the heat shock protein 70 family. Mol Cell Biol 13:3598–3610PubMed

Dou F, Netzer WJ, Tanemura K, Li F, Hartl FU, Takashima A, Gouras GK, Greengard P, Xu H (2003) Chaperones increase association of tau protein with microtubules. Proc Natl Acad Sci USA 100:721–726PubMedCrossRef

Eriksson M, Jokinen E, Sistonen L, Leppa S (2000) Heat shock factor 2 is activated during mouse heart development. Int J Dev Biol 44:171–477

Fiorenza MT, Farkas T, Dissing M, Kolding D, Zimarino V (1995) Complex expression of murine heat shock transcription factors. Nucleic Acids Res 23:467–474PubMedCrossRef

Giebel LB, Dworniczak BP, Bautz EK (1988) Developmental regulation of a constitutively expressed mouse mRNA encoding a 72-kDa heat shock-like protein. Dev Biol 125:200–207PubMedCrossRef

Gullo CA, Teoh G (2004) Heat shock proteins: to present or not, that is the question. Immunol Lett 94:1–10PubMedCrossRef

Huang L, Mivechi NF, Moskophidis D (2001) Insights into regulation and function of the major stress-induced hsp70 molecular chaperone in vivo: analysis of mice with targeted gene disruption of the hsp70.1 or hsp70.3 gene. Mol Cell Biol 21:8575–8591PubMedCrossRef

Hunt CR, Gasser DL, Chaplin DD, Pierce JC, Kozak CA (1993) Chromosomal localization of five murine HSP70 gene family members: Hsp70–1, Hsp70-2, Hsp70-3, Hsc70t, and Grp78. Genomics 16:193–198PubMedCrossRef

Huszar G, Stone K, Dix D, Vigue L (2000) Putative creatine kinase M-isoform in human sperm is identified as the 70-kilodalton heat shock protein HspA2. Biol Reprod 63:925–32PubMedCrossRef

Ikawa M, Yamada S, Nakanishi T, Okabe M (1999) Green fluorescent protein (GFP) as a vital marker in mammals. Curr Top Dev Biol 44:1–20PubMedCrossRef

Kalio M, Chang Y, Manuel M, Alastalo T, Rallau M, Gitton Y, Pirkalla L, Loones M, Paslaru L, Larney S, Hiard S, Morange M, Sistonen L, Mezger L (2002) Brain abnormalities, defective meiotic chromosome synapsis and female subfertility in HSF2 null mice. EMBO J 21:2591–2601CrossRef

Kozutsumi Y, Normington K, Press E, Slaughter C, Sambrook J, Gething MJ (1989) Identification of immunoglobulin heavy chain binding protein as glucose-regulated protein 78 on the basis of amino acid sequence, immunological cross-reactivity, and functional activity. J Cell Sci Suppl 11:115–137PubMed

Krawczyk Z, Wisniewski J, Biesiada E (1987) A hsp70-related gene is constitutively highly expressed in testis of rat and mouse. Mol Biol Rep 12:27–34PubMedCrossRef

Krawczyk Z, Mali P, Parvinen M (1988) Expression of testis-specific hsp70 gene-related RNA in defined stages of rat seminiferous epithelium. J Cell Biol 107:1317–1323PubMedCrossRef

Li GC, Mivechi NF (1986) Thermotolerance in mamalian systems: a review, vol 1. CRC Press, Boca Raton

Loones MT, Morange M (1998) Hsp and chaperone distribution during endochondral bone development in mouse embryo. Cell Stress Chaperones 3:237–244PubMedCrossRef

Loones MT, Rallu M, Mezger V, Morange M (1997) HSP gene expression and HSF2 in mouse development. Cell Mol Life Sci 53:179–190PubMedCrossRef

Marshal OJ, Harley RV (2001) Identification of an interaction between SOX9 and HSP70. FEBS Lett 496:75–80CrossRef

Matsumoto M, Fujimoto H (1990) Cloning of a hsp70-related gene expressed in mouse spermatids. Biochem Biophys Res Commun 166:43–49PubMedCrossRef

Matwee Ch, Kamaruddin M, Betts DH, Basrur PK, King WA (2001) The effects of antibodies to heat shock protein 70 in fertilization and embryo development. Mol Hum Reprod 7:829–837CrossRef

Morange M, Diu A, Bensaude O, Babinet C (1984) Altered expression of heat shock proteins in embryonal carcinoma and mouse early embryonic cells. Mol Cell Biol 4:730–735PubMed

Moriya M, Ochiai M, Yuasa HJ, Suzuki N, Yazawa M (2004) Identification of Ca2+-dependent calmodulin-binding proteins in rat spermatogenic cells as complexes of the heat-shock proteins. Mol Reprod Dev 69:316–324PubMedCrossRef

Murashov AK, Wolgemuth DJ (1996) Sense and antisense transcripts of the developmentally regulated murine hsp70.2 gene are expressed in distinct and only partially overlapping areas in the adult brain. Brain Res Mol Brain Res 37:85–95PubMedCrossRef

Murphy SP, Gorzowski JJ, Sarge KD, Phillips B (1994) Characterization of constitutive HSF2 DNA-binding activity in mouse embryonal carcinoma cells. Mol Cell Biol 14:5309–5317PubMed

Parsell DA, Lindquist S (1993) The function of heat-shock proteins in stress tolerance: degradation and reactivation of damaged proteins. Annu Rev Genet 27:437–496PubMedCrossRef

Pikaart MJ, Recillas-Targa F, Felsenfeld G (1998) Loss of transcriptional activity of a transgene is accompanied by DNA methylation and histone deacetylation and is prevented by insulators. Genes Dev 12:2852–2862PubMedCrossRef

Pratt WB, Galigniana MD, Morishima Y, Murphy PJ (2004) Role of molecular chaperones in steroid receptor action. Essays Biochem 40:41–58PubMed

Rallu M, Loones M, Lallemand Y, Morimoto R, Morange M, Mezger V (1997) Function and regulation of heat shock factor 2 during mouse embryogenesis. Proc Natl Acad Sci USA 94:2392–2397PubMedCrossRef

Recillas-Targa F, Pikaart MJ, Burgess-Beusse B, Bell AC, Litt MD, West AG, Gaszner M, Felsenfeld G (2002) Position-effect protection and enhancer blocking by the chicken beta-globin insulator are separable activities. Proc Natl Acad Sci USA 99:6883–6888PubMedCrossRef

Reddi PP, Kallio M, Herr JC (1999) Green fluorescent protein as a reporter for promoter analysis of testis-specific genes in transgenic mice. Methods Enzymol 302:272–284PubMedCrossRef

Sarge KD, Park-Sarge OK, Kirby JD, Mayo KE, Morimoto RI (1994) Expression of heat shock factor 2 in mouse testis: potential role as a regulator of heat-shock protein gene expression during spermatogenesis. Biol Reprod 50:1334–1343PubMedCrossRef

Ścieglińska D, Widłak W, Rusin M, Markkula M, Krawczyk Z (1997) Expression of the testis-specific HSP70-related gene (HST70) gene in somatic non-testicular rat tissues revealed by RT-PCR and transgenic mice analysis. Cell Biol Int 21:813–821PubMedCrossRef

Trichilis A, Wróblewski J (1997) Expression of p53 and Hsp70 in relation to apoptosis during Meckel’s cartilage development in the mouse. Anat Embryol (Berl) 196:107–113CrossRef

Vanmuylder N, Werry-Huet A, Rooze M, Louryan S (2002) Heat shock protein HSP86 expression during mouse embryo development, especially in the germ-line. Anat Embryol 205:301–306PubMedCrossRef

Voss AK, Thomas T, Gruss P (2000) Mice lacking HSP90β fail to develop a placental labyrinth. Development 127:1–11PubMed

Walter I, Fleischmann M, Klein D, Muller M, Salmons B, Gunzburg WH, Renner M, Gelbmann W (2000) Rapid and sensitive detection of enhanced green fluorescent protein expression in paraffin sections by confocal laser scanning microscopy. Histochem J 32(2):99–103PubMedCrossRef

Widłak W, Markkula M, Krawczyk Z, Huhtaniemi I (1994) Functional analysis of spermatocyte-specific hst70 gene promoter in transgenic mice. Acta Biochim Pol 41:103–105PubMed

Widłak W, Markkula M, Krawczyk Z, Kananen K, Huhtaniemi I (1995) A 252 bp upstream region of the rat spermatocyte-specific hst70 gene is sufficient to promote expression of the hst70-CAT hybrid gene in testis and brain of transgrnic mice. Biochim Biophys Acta 1264:191–200PubMed

Widłak W, Scieglinska D, Vydra N, Malusecka E, Krawczyk Z (2003) In vivo electroporation of the testis versus transgenic mice model in functional studies of spermatocyte-specific hst70 gene promoter: a comparative study. Mol Reprod Dev 65:382–388PubMedCrossRef

Zakeri ZF, Wolgemuth DJ, Hunt CR (1988) Identification and sequence analysis of a new member of the mouse HSP70 gene family and characterization of its unique cellular and developmental pattern of expression in the male germ line. Mol Cell Biol 8:2925–2932PubMed

Zhu D, Dix DJ, Eddy EM (1997) HSP70-2 is required for CDC2 kinase activity in meiosis I of mouse spermatocytes. Development 124:3007–3014PubMed

Titel: Promoter of the heat shock testis-specific Hsp70.2/Hst70 gene is active in nervous system during embryonic development of mice
verfasst von: Weronika Rupik
Agata Stawierej
Iwona Stolarczyk
Wieslawa Widłak
Publikationsdatum: 01.11.2006
Verlag: Springer-Verlag
Erschienen in: Brain Structure and Function / Ausgabe 6/2006
Print ISSN: 1863-2653
Elektronische ISSN: 1863-2661
DOI: https://doi.org/10.1007/s00429-006-0125-x

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Promoter of the heat shock testis-specific Hsp70.2/Hst70 gene is active in nervous system during embryonic development of mice

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