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Journal of Natural Medicines

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10.05.2016 | Original Paper

Protective effect of Mimosa pudica L. in an l-arginine model of acute necrotising pancreatitis in rats

verfasst von: Jagdeep Kaur, Shabir Sidhu, Kanwaljit Chopra, M. U. Khan

Erschienen in: Journal of Natural Medicines | Ausgabe 3/2016

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Abstract

Mimosa pudica is used in traditional medicine for treating various disorders such as inflammatory conditions, diarrhoea, insomnia, alopecia, urogenital infections and wounds. The present study investigated the effect of M. pudica extract (MPE) on l-arginine-induced acute necrotising pancreatitis in rats. The ethanolic extract of M. pudica leaves was studied for the presence of quercetin and gallic acid using high-performance liquid chromatography. Four groups were employed—normal control rats, l-arginine control rats (two intraperitoneal [i.p.] injections of 2 g/kg at an interval of 1 h), MPE-treated rats (400 mg/kg orally) and melatonin-treated rats (positive control 10 mg/kg i.p.), which were further divided into subgroups according to time points (24 h, 3 days and 14 days). Serum amylase, lipase, tumour necrosis factor-α (TNF-α), pancreatic amylase, nucleic acid content, protein, transforming growth factor-β1 (TGF-β1), thiobarbituric reactive substances, glutathione, nitrite/nitrate, collagen content and histopathological examination were carried out. MPE significantly improved acute necrotising pancreatitis by modulating diagnostic markers of pancreatitis such as serum lipase and pancreatic amylase, inflammation (TNF-α), and oxidative and nitrosative stress. Moreover, MPE administration induced regenerative changes in the pancreas evidenced by increased levels of pancreatic proteins, nucleic acid content and histopathology report. In addition, MPE improved TGF-β1 and collagen levels thereby preventing fibrosis. The current investigation indicates the novel role of MPE in reducing the severity of acute necrotising pancreatitis by plausible mechanisms such as anti-inflammatory and anti-fibrotic activity and by promoting repair and regeneration of the pancreas.

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Titel: Protective effect of Mimosa pudica L. in an l-arginine model of acute necrotising pancreatitis in rats
verfasst von: Jagdeep Kaur
Shabir Sidhu
Kanwaljit Chopra
M. U. Khan
Publikationsdatum: 10.05.2016
Verlag: Springer Japan
Erschienen in: Journal of Natural Medicines / Ausgabe 3/2016
Print ISSN: 1340-3443
Elektronische ISSN: 1861-0293
DOI: https://doi.org/10.1007/s11418-016-0991-3

Springer Medizin

Abstract

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