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International Ophthalmology

Erschienen in:

08.01.2020 | Original Paper

Protective effects of autophagy inhibitor 3-methyladenine on ischemia–reperfusion-induced retinal injury

verfasst von: Xiaorui Wang, Yuyu Wu

Erschienen in: International Ophthalmology | Ausgabe 5/2020

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Abstract

Objective

To investigate the protective effects of autophagy inhibitor 3-methyladenine (3-MA) in a rat model of ischemic–reperfusion injury (IRI).

Methods

Forty Sprague–Dawley male rats (weight 220–250 g) were randomly divided into four groups: a control group (NC, n = 10), a Sham surgery group (n = 10), an IRI group (n = 10), and a 3-MA-treated IRI group [10 μL 3-MA (10 mmol/L) was injected in vitreous after the injury, n = 10]. The retinal IRI was induced by elevating the intraocular pressure to 110 mmHg for 60 min. Hematoxylin and eosin (HE) staining was used to calculate the number of retinal ganglion cells (RGCs). The level of microtubule-associated protein 1A/1B light chain 3 (LC3), Beclin-1, and Caspase-3 in the retina was detected using the immunofluorescence staining method. The LC3, Beclin-1, B-cell lymphoma/leukemia-2 (Bcl-2), and Caspase-3 protein levels were examined by Western blotting.

Results

The number of RGCs in IRI group was significantly lower than that in NC group (P < 0.05), demonstrated by HE staining. Western blotting results indicated that the protein expression of LC3 and Beclin-1 in the IRI group was significantly elevated compared with those in the NC group (P < 0.05). However, with 3-MA treatment, the number of RCGs in 3-MA-treated IRI group was elevated and protein levels of LC3, Beclin-1 were down-regulated, compared with those in the IRI group (P < 0.05). Further immunohistochemistry staining and Western blot showed that 3-MA-treated IRI group presented down-regulated Caspase-3 and up-regulated Bcl-2 protein expression with comparison of IRI group (P < 0.05).

Conclusions

Retina IRI-caused RGCs loss involved activated autophagy pathway and apoptosis, which could be prevented by autophagy inhibitor 3-MA. Autophagy inhibitor 3-MA may act as a potent therapeutic tool in attenuating retina IRI.

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Titel: Protective effects of autophagy inhibitor 3-methyladenine on ischemia–reperfusion-induced retinal injury
verfasst von: Xiaorui Wang
Yuyu Wu
Publikationsdatum: 08.01.2020
Verlag: Springer Netherlands
Erschienen in: International Ophthalmology / Ausgabe 5/2020
Print ISSN: 0165-5701
Elektronische ISSN: 1573-2630
DOI: https://doi.org/10.1007/s10792-019-01272-9

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Springer Medizin

Protective effects of autophagy inhibitor 3-methyladenine on ischemia–reperfusion-induced retinal injury

Abstract

Objective

Methods

Results

Conclusions

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Ophthalmika in der Schwangerschaft

Operative Therapie und Keimnachweis bei endogener Endophthalmitis

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Update Augenheilkunde

Springer Medizin

Abstract

Objective

Methods

Results

Conclusions

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