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Graefe's Archive for Clinical and Experimental Ophthalmology

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01.05.2004 | Short Communication

Protein expression pattern of collagen type XV in mouse cornea

verfasst von: Shizuya Saika, Yuka Okada, Takeshi Miyamoto, Osamu Yamanaka, Yoshitaka Ohnishi, Akio Yamanaka, Akira Ooshima

Erschienen in: Graefe's Archive for Clinical and Experimental Ophthalmology | Ausgabe 5/2004

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Abstract

Purpose

To examine the alteration of protein expression pattern of collagen type XV in cornea during embryonic development and adult tissue repair. Collagen type XV is a basement membrane collagen of a subfamily of multiplexins (multiple triple helix domains and interruptions). Its COOH-terminal peptide has an anti-angiogenic effect and its distribution in avascular tissue of cornea is of interest.

Methods

Eyes of mouse embryos [day (E) 10.5–18.5] and healing adult mouse corneas following either débridement injury or incision were embedded in paraffin. Deparaffinized sections were processed for immunofluorescent staining with anti-collagen XV antibody.

Results

At E14.5 embryonic corneal epithelium, as well as fibroblasts in eyelids, began to express this collagen type very faintly, and at E18.5, besides corneal epithelial expression, epidermis, palpebral conjunctiva, and keratocytes started to express collagen type XV. In adult mouse cornea, collagen type XV was observed in basal and suprabasal epithelial cells and stroma, but not in the subepithelial basement membrane. Healing epithelial cells following débridement or incision injury down-regulated its protein expression.

Conclusions

Mouse embryonic corneal epithelium and keratocytes begin to express collagen type XV before birth. Healing murine corneal epithelium down-regulates collagen XV expression. The presence of collagen XV in corneal stroma may play a part in avascularity.

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Titel: Protein expression pattern of collagen type XV in mouse cornea
verfasst von: Shizuya Saika
Yuka Okada
Takeshi Miyamoto
Osamu Yamanaka
Yoshitaka Ohnishi
Akio Yamanaka
Akira Ooshima
Publikationsdatum: 01.05.2004
Verlag: Springer-Verlag
Erschienen in: Graefe's Archive for Clinical and Experimental Ophthalmology / Ausgabe 5/2004
Print ISSN: 0721-832X
Elektronische ISSN: 1435-702X
DOI: https://doi.org/10.1007/s00417-003-0854-y

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Springer Medizin

Protein expression pattern of collagen type XV in mouse cornea

Abstract

Purpose

Methods

Results

Conclusions

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Springer Medizin

Abstract

Purpose

Methods

Results

Conclusions

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