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Netherlands Heart Journal

Erschienen in:

01.05.2009 | original article

Renal failure induces telomere shortening in the rat heart

verfasst von: L. S. Wong, W. A. Windt, A. J. Roks, R. P. van Dokkum, R. G. Schoemaker, D. de Zeeuw, R. H. Henning

Erschienen in: Netherlands Heart Journal | Ausgabe 5/2009

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Abstract

BackgroundRenal failure aggravates pathological cardiac remodelling induced by myocardial infarction (MI). Cardiac remodelling is associated with telomere shortening, a marker for biological ageing. We investigated whether mild and severe renal failure shorten cardiac telomeres and excessively shorten telomeres after MI.

MethodsRats were subjected to sham, unilateral (UNX) or 5/6th nephrectomy (5/6NX) to induce none, mild or severe renal failure. MI was induced by left coronary artery ligation. Renal function parameters and blood pressure were measured. DNA was isolated from non-infarcted cardiac tissue. Telomere length was assessed by quantitative polymerase chain reaction (PCR).

ResultsProteinuria was unchanged in UNX and MI compared with control, but strongly increased in 5/6NX, UNX+MI and 5/6NX+MI. Serum creatinine levels were increased fourfold in 5/6NX and tenfold in 5/6NX+MI. 5/6NX and groups with both renal failure and MI showed an approximate 20% reduction of telomere length, similar to the MI group. No excess telomere shortening was observed in hearts from rats with renal ablation after MI.

ConclusionSevere renal failure, but not mild renal failure, leads to shortening of cardiac telomeres to a similar extent as found after MI. Renal failure did not induce excessive telomere shortening after MI. (Neth Heart J 2009;17:190–4.)

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Titel: Renal failure induces telomere shortening in the rat heart
verfasst von: L. S. Wong
W. A. Windt
A. J. Roks
R. P. van Dokkum
R. G. Schoemaker
D. de Zeeuw
R. H. Henning
Publikationsdatum: 01.05.2009
Verlag: Bohn Stafleu van Loghum
Erschienen in: Netherlands Heart Journal / Ausgabe 5/2009
Print ISSN: 1568-5888
Elektronische ISSN: 1876-6250
DOI: https://doi.org/10.1007/BF03086245

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