Background
Methods
Animals
Welding fume collection and characterization
Welding fume preparation
Mouse pharyngeal aspiration exposure
Body weight determination
Whole lung RNA isolation
MouseRef-8 v1.1 Illumina BeadChips
Statistics and data analysis strategy for Illumina beadchips
Hierarchical clustering
Molecular Network Analysis using Ingenuity Pathways Analysis (IPA)
Confirmation of microarray data by RT-qPCR
Results and Discussion
Hierarchical clustering
Gene activation 4 and 16 weeks post-exposure to welding fume
4 weeks post-exposure to GMA-MS: IPA analysis
16 weeks post-exposure to GMA-MS: IPA analysis
Summary of network discovery after GMA-MS welding fume exposure
4 weeks post-exposure to GMA-SS: IPA analysis
16 weeks post-exposure to GMA-SS: IPA analysis
Summary of network discovery after GMA-SS welding fume exposure
Functional analysis of the lung response after GMA-MS and GMA-SS exposure
Strain | Diseases and disorders | p-valueb
| # of genes (up,down) |
---|---|---|---|
A/J | Connective tissue disorders | 5.92E-07 - 3.04E-02 | 10 (10,0) |
Immunological disease | 5.92E-07 - 3.39E-02 | 11 (11,0) | |
Inflammatory disease | 5.92E-07 - 3.11E-02 | 11 (11,0) | |
Skeletal and muscular disorders | 5.92E-07 - 3.04 E-02 | 11 (11,0) | |
Organismal injury and abnormalities | 6.90E-05 - 8.58E-03 | 6 (5,1) | |
B6 | Cancer | 9.46E-10 - 2.51E-03 | 14 (3,11) |
Cardiovascular disease | 1.13E-08 - 1.25E-03 | 9 (2,7) | |
Connective tissue disorders | 3.71E-08 - 2.51E-03 | 8 (1,7) | |
Immunological disease | 3.71E-08 - 2.51E-03 | 9 (1,8) | |
Inflammatory disease | 3.71E-08 - 1.32E-03 | 8 (1,7) |
Strain
|
Molecular and Cellular Functions
|
p-value
b
|
# of genes (up,down)
|
---|---|---|---|
A/J | Cellular movement | 2.41E-05 - 3.28E-02 | 14 (11,3) |
Cell-to-cell signaling and interaction | 5.91E-04 - 3.28E-02 | 9 (6,3) | |
Post-translational modification | 6.16E-04 - 1.54E-02 | 5 (4,1) | |
Cellular development | 9.42E-04 - 3.39E-02 | 7 (5,2) | |
Cell death | 1.24E-03 - 3.39E-02 | 13 (9,4) | |
B6 | Cell death | 9.46E-10 - 2.60E-03 | 14 (3,11) |
Cellular movement | 4.44E-08 - 2.51E-03 | 11 (2,9) | |
Cellular development | 5.74E-08 - 2.51E-03 | 11 (2,9) | |
Cellular growth and proliferation | 1.52E-07 - 2.51E-03 | 15 (3,12) | |
Cell cycle | 1.52E-07 - 2.54E-03 | 7 (0,7) |
Strain
|
Physiological System Development and Function
|
p-value
b
|
# of genes (up,down)
|
---|---|---|---|
A/J | Hematological system development and function | 2.41E-05 - 3.37E-02 | 14 (10,4) |
Immune response | 4.67E-05 - 3.22E-02 | 14 (11,3) | |
Behavior | 3.57E-04 - 3.95E-04 | 3 (3,0) | |
Nervous system development/function | 3.57E-04 - 3.11E-02 | 7 (7,0) | |
Cardiovascular system development/function | 2.87E-03 - 3.28E-02 | 5 (3,2) | |
B6 | Tissue morphology | 1.63E-06 - 2.51E-03 | 9 (0,9) |
Connective tissue development/function | 8.69E-06 - 2.51E-03 | 8 (0,8) | |
Skeletal and muscular system development/function | 1.50E-05 - 2.57E-03 | 9 (1,8) | |
Cardiovascular system development/function | 2.03E-05 - 2.51E-03 | 7 (2,5) | |
Nervous system development/function | 2.34E-05 - 2.51E-03 | 6 (1,5) |
Strain | Diseases and disorders | p-valueb
| # of genes (up,down) |
---|---|---|---|
A/J | Cancer | 2.14E-04 - 4.71E-02 | 19 (7,12) |
Gastrointestinal disease | 2.14E-04 - 3.72E-02 | 6 (1,5) | |
Renal and urological disease | 9.37E-04 - 3.18E-02 | 4 (1,3) | |
Cardiovascular disease | 2.69E-03 - 4.73E-02 | 4 (2,2) | |
Connective tissue disorders | 2.69E-03 - 5.37E-03 | 1 (0,1) | |
B6 | Connective tissue disorders | 4.66E-04 - 5.37E-03 | 4 (3,1) |
Immunological disease | 4.66E-04 - 4.11E-02 | 5 (4,1) | |
Inflammatory disease | 4.66E-04 - 3.08E-02 | 4 (3,1) | |
Skeletal and muscular disorders | 4.66E-04 - 2.76E-02 | 4 (3,1) | |
Cancer | 4.99E-04 - 3.91E-02 | 7 (5,2) |
Strain
|
Molecular and cellular functions
|
p-value
b
|
# of genes (up,down)
|
---|---|---|---|
A/J | Cellular compromise | 1.03E-06 - 1.03E-06 | 4 (0,4) |
Cellular function and maintenance | 9.60E-06 - 3.44E-02 | 8 (3,5) | |
Gene expression | 2.12E-05 - 4.98E-02 | 9 (3,6) | |
Cell death | 6.95E-05 - 4.73E-02 | 17 (8,9) | |
Cellular development | 2.72E-04 - 4.99E-02 | 13 (6,7) | |
B6 | Cellular movement | 7.17E-05 - 4.11E-02 | 5 (4,1) |
Cell-to-cell signaling and interaction | 5.22E-04 - 4.08E-02 | 5 (5,0) | |
Cell morphology | 1.08E-03 - 3.60E-02 | 4 (3,1) | |
Cellular assembly and organization | 1.08E-03 - 1.92E-02 | 3 (1,2) | |
Cellular development | 1.08E-03 - 4.11E-02 | 7 (5,2) |
Strain
|
Physiological System Development and Function
|
p-value
b
|
# of genes (up,down)
|
---|---|---|---|
A/J | Organismal development | 2.54E-04 - 4.99E-02 | 9 (4,5) |
Connective tissue development/function | 2.05E-03 - 4.99E-02 | 7 (1,6) | |
Tissue morphology | 2.05E-03 - 4.53E-02 | 10 (4,6) | |
Digestive system development/function | 2.69E-03 - 4.48E-02 | 3 (0,3) | |
Embryonic development | 2.69E-03 - 4.99E-02 | 6 (3,3) | |
B6 | Behavior | 2.09E-07 - 4.89E-05 | 4 (2,2) |
Nervous system development/function | 2.09E-07 - 3.80E-02 | 7 (5,2) | |
Hematological system development/function | 7.17E-05 - 3.70E-02 | 5 (5,0) | |
Immune response | 7.17E-05 - 4.01E-02 | 5 (5,0) | |
Tumor morphology | 4.99E-04 - 2.76E-02 | 2 (2,0) |
Strain | Diseases and disorders | p-valueb
| # of genes (up,down) |
---|---|---|---|
A/J | Connective tissue disorders | 3.84E-15 - 5.61E-04 | 17 (17,0) |
Immunological disease | 3.84E-15 - 2.50E-03 | 20 (20,0) | |
Inflammatory disease | 3.84E-15 - 2.82E-03 | 19 (19,0) | |
Skeletal and muscular disorders | 3.84E-15 - 3.04E-03 | 19 (18,1) | |
Cancer | 5.03E-07 - 3.05E-03 | 24 (20,4) | |
B6 | Inflammatory disease | 5.17E-09 - 1.95E-03 | 12 (3,9) |
Connective tissue disorders | 2.08E-08 - 1.95E-03 | 9 (1,8) | |
Skeletal and muscular disorders | 2.08E-08 - 1.95E-03 | 11 (3,8) | |
Immunological disease | 2.80E-08 - 4.08E-04 | 11 (2,9) | |
Hematological disease | 2.76E-07 - 1.49E-03 | 8 (1,8) |
Strain
|
Molecular and cellular functions
|
p-value
b
|
# of genes (up,down)
|
---|---|---|---|
A/J | Cellular movement | 2.53E-13 - 2.94E-03 | 17 (17,0) |
Cell-to-cell signaling and interaction | 5.99E-10 - 3.05E-03 | 17 (16,1) | |
Cell morphology | 9.53E-09 - 2.82E-03 | 12 (12,0) | |
Cellular assembly and organization | 5.30E-07 - 2.33E-04 | 6 (6,0) | |
Cell signaling | 7.86E-07 - 2.05E-03 | 12 (12,0) | |
B6 | Cellular movement | 3.03E-09 - 2.04E-03 | 11 (2,9) |
Cellular development | 2.76E-07 - 1.90E-03 | 13 (4,9) | |
Cell-to-cell signaling and interaction | 3.41E-07 - 1.49E-03 | 10 (2,8) | |
Cell cycle | 3.78E-07 - 2.13E-03 | 7 (1,6) | |
Post-translational modification | 8.34E-07 - 1.49E-03 | 4 (2,2) |
Strain
|
Physiological System Development and Function
|
p-value
b
|
# of genes (up,down)
|
---|---|---|---|
A/J | Hematological system development/function | 2.53E-13 - 3.04E-03 | 21 (21,0) |
Immune response | 2.53E-13 - 2.28E-03 | 23 (23,0) | |
Immune and lymphatic system development/function | 5.99E-10 - 3.04E-03 | 19 (19,0) | |
Tissue development | 2.83E-07 - 3.05E-03 | 11 (10,1) | |
Tissue morphology | 2.28E-06 - 9.11E-04 | 10 (10,0) | |
B6 | Hematological system development/function | 3.03E-09 - 1.53E-03 | 11 (2,9) |
Immune response | 3.03E-09 - 1.49E-03 | 10 (2,8) | |
Immune and lymphatic system development/function | 8.99E-09 - 1.53E-03 | 10 (2,8) | |
Tissue morphology | 8.99E-09 - 1.53E-03 | 10 (2,8) | |
Tissue development | 1.46E-08 - 1.49E-03 | 11 (3,8) |
Strain | Diseases and disorders | p-valueb
| # of genes (up,down) |
---|---|---|---|
A/J | Inflammatory disease | 5.57E-06 - 7.31E-03 | 12 (8,4) |
Connective tissue disorders | 2.88E-05 - 5.13E-03 | 8 (7,1) | |
Immunological disease | 2.88E-05 - 7.69E-03 | 10 (9,1) | |
Skeletal and muscular disorders | 2.88E-05 - 7.69E-03 | 7 (7,0) | |
Cancer | 9.61E-05 - 7.69E-03 | 21 (14,7) | |
B6 | Connective tissue disorders | 1.29E-08 - 5.01E-03 | 8 (7,1) |
Inflammatory disease | 1.29E-08 - 5.01E-03 | 8 (7,1) | |
Skeletal and muscular disorders | 1.29E-08 - 3.58E-03 | 7 (7,0) | |
Immunological disease | 2.26E-08 - 5.01E-03 | 8 (7,1) | |
Hematological disease | 8.16E-07 - 3.58E-03 | 6 (5,1) |
Strain
|
Molecular and cellular functions
|
p-value
b
|
# of genes (up,down)
|
---|---|---|---|
A/J | Cell morphology | 6.31E-08 - 7.69E-03 | 9 (7,2) |
Cell cycle | 3.91E-06 - 7.59E-03 | 8 (5,3) | |
Cell-to-cell signaling and interaction | 6.45E-06 - 7.69E-03 | 11 (8,3) | |
Cellular function and maintenance | 6.45E-06 - 5.13E-03 | 6 (3,3) | |
Cellular growth and proliferation | 6.45E-06 - 7.69E-03 | 15 (10,5) | |
B6 | Cellular movement | 6.85E-10 - 5.01E-03 | 8 (7,1) |
Cell-to-cell signaling and interaction | 1.37E-08 - 4.52E-03 | 7 (6,1) | |
Cellular growth and proliferation | 5.17E-07 - 4.96E-03 | 9 (7,2) | |
Lipid metabolism | 1.41E-06 - 3.44E-04 | 2 (2,0) | |
Molecular transport | 1.41E-06 - 3.32E-03 | 4 (4,0) |
Strain
|
Physiological System Development and Function
|
p-value
b
|
# of genes (up,down)
|
---|---|---|---|
A/J | Hematological system development/function | 6.31E-08 - 7.69E-03 | 13 (10,3) |
Immune and lymphatic system development/function | 6.45E-06 - 7.69E-03 | 13 (10,3) | |
Immune response | 1.21E-05 - 7.69E-03 | 13 (11,2) | |
Organismal development | 3.08E-05 - 7.50E-03 | 8 (5,3) | |
Embryonic development | 3.86E-05 - 5.13E-03 | 3 (3,0) | |
B6 | Hematological system development/function | 2.25E-10 - 5.01E-03 | 7 (6,1) |
Immune response | 2.25E-10 - 5.01E-03 | 7 (6,1) | |
Immune and lymphatic system development/function | 2.25E-10 - 4.30E-03 | 7 (6,1) | |
Tissue development | 2.25E-10 - 4.30E-03 | 7 (6,1) | |
Tissue morphology | 3.09E-08 - 4.30E-03 | 7 (6,1) |