This study aimed at investigating the relative roles of the duration versus intensity of exercise on the metabolic adaptations in vastus lateralis to short-term (10 day) aerobic-based cycle training. Healthy males with a peak aerobic power (
\( {\dot{\text{V}}\text{O}}_{{ 2 {\text{peak}}}} \)) of 46.0 ± 2.0 ml kg
−1 min
−1 were assigned to either a 30-min (
n = 7) or a 60-min (
n = 8) duration performed at two different intensities (with order randomly assigned), namely moderate (M) and heavy (H), corresponding to 70 and 86 %
\( {\dot{\text{V}}\text{O}}_{{ 2 {\text{peak}}}} \), respectively. No change (
P > 0.05) in
\( {\dot{\text{V}}\text{O}}_{{ 2 {\text{peak}}}} \) was observed regardless of the training program. Based on the metabolic responses to prolonged exercise (60 %
\( {\dot{\text{V}}\text{O}}_{{ 2 {\text{peak}}}} \)), both M and H and 30 and 60 min protocols displayed less of a decrease (
P < 0.05) in phosphocreatine (PCr) and glycogen (Glyc) and less of an increase (
P < 0.05) in free adenosine diphosphate (ADP
f), free adenosine monophosphate (AMP
f), inosine monophosphate (IMP) and lactate (La). Training for 60 min compared with 30 min resulted in a greater protection (
P < 0.05) of ADP
f, AMP
f, PCr and Glyc during exercise, effects that were not displayed between M and H. The reduction in both
\( {\dot{\text{V}}\text{O}}_{ 2} \) and RER (
P < 0.05) observed during submaximal exercise did not depend on training program specifics. These findings indicate that in conjunction with our earlier study (Green et al., Eur J Appl Physiol,
2012b), a threshold exists for duration rather than intensity of aerobic exercise to induce a greater training impact in reducing metabolic strain.