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Erschienen in: Forensic Science, Medicine and Pathology 2/2015

01.06.2015 | Original Article

Seasonal variation of postmortem microbial communities

verfasst von: David O. Carter, Jessica L. Metcalf, Alexander Bibat, Rob Knight

Erschienen in: Forensic Science, Medicine and Pathology | Ausgabe 2/2015

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Abstract

Body-associated microbes were recently shown to change significantly during decomposition, undergoing an ecological succession in experimental conditions using rodent and swine models. We investigated microbial succession in soils associated with swine carcasses under experimental field conditions in summer and winter. We demonstrate that these postmortem microbial communities change in a specific, reproducible fashion, and that soil microbes represent a significant component of the postmortem microbial community, contrary to widespread belief in forensic science. However, the effects of decomposition on soil microbial communities were different in summer and winter. We suggest that the microbial ecological succession will be useful in medicolegal death investigation; however, observations in winter might not be applicable to summer, which indicates a need for a greater understanding of the seasonality of decomposition.
Literatur
1.
Zurück zum Zitat Metcalf J, Wegener-Parfrey L, Gonzalez A, Lauber CL, Knights D, Ackermann G, et al. A microbial clock provides an accurate estimate of the postmortem interval in a mouse model system. eLife. 2013;2:e01104.CrossRefPubMedCentralPubMed Metcalf J, Wegener-Parfrey L, Gonzalez A, Lauber CL, Knights D, Ackermann G, et al. A microbial clock provides an accurate estimate of the postmortem interval in a mouse model system. eLife. 2013;2:e01104.CrossRefPubMedCentralPubMed
2.
Zurück zum Zitat Pechal JL, Crippen TL, Benbow ME, Tarone AM, Dowd S, Tomberlin JK. The potential use of bacterial community succession in forensics as described by high throughput metagenomic sequencing. Int J Leg Med. 2014;128:193–205.CrossRef Pechal JL, Crippen TL, Benbow ME, Tarone AM, Dowd S, Tomberlin JK. The potential use of bacterial community succession in forensics as described by high throughput metagenomic sequencing. Int J Leg Med. 2014;128:193–205.CrossRef
3.
Zurück zum Zitat Hyde ER, Haarmann DP, Lynne AM, Bucheli SR, Pertrosino JF. The living dead: bacterial community structure of a cadaver at the onset and end of the bloat stage of decomposition. PLoS One. 2013;8:e77733.CrossRefPubMedCentralPubMed Hyde ER, Haarmann DP, Lynne AM, Bucheli SR, Pertrosino JF. The living dead: bacterial community structure of a cadaver at the onset and end of the bloat stage of decomposition. PLoS One. 2013;8:e77733.CrossRefPubMedCentralPubMed
4.
Zurück zum Zitat Pechal JL, Crippen TL, Tarone AM, Lewis AJ, Tomberlin JK, Benbow ME. Microbial community functional change during vertebrate carrion decomposition. PLoS One. 2013;8:e79035.CrossRefPubMedCentralPubMed Pechal JL, Crippen TL, Tarone AM, Lewis AJ, Tomberlin JK, Benbow ME. Microbial community functional change during vertebrate carrion decomposition. PLoS One. 2013;8:e79035.CrossRefPubMedCentralPubMed
5.
Zurück zum Zitat Vass AA. The elusive universal post-mortem interval formula. Forensic Sci Int. 2011;204:34–40.CrossRefPubMed Vass AA. The elusive universal post-mortem interval formula. Forensic Sci Int. 2011;204:34–40.CrossRefPubMed
6.
Zurück zum Zitat Carter DO, Yellowlees D, Tibbett M. Cadaver decomposition in terrestrial ecosystems. Naturwissenschaften. 2007;94:12–24.CrossRefPubMed Carter DO, Yellowlees D, Tibbett M. Cadaver decomposition in terrestrial ecosystems. Naturwissenschaften. 2007;94:12–24.CrossRefPubMed
7.
Zurück zum Zitat Carter DO, Yellowlees D, Tibbett M. Temperature affects microbial decomposition of cadavers (Rattus rattus) in contrasting soils. Appl Soil Ecol. 2008;40:129–37.CrossRef Carter DO, Yellowlees D, Tibbett M. Temperature affects microbial decomposition of cadavers (Rattus rattus) in contrasting soils. Appl Soil Ecol. 2008;40:129–37.CrossRef
8.
Zurück zum Zitat Carter DO, Yellowlees D, Tibbett M. Moisture can be the dominant environmental parameter governing cadaver decomposition in soil. Forensic Sci Int. 2010;200:60–6.CrossRefPubMed Carter DO, Yellowlees D, Tibbett M. Moisture can be the dominant environmental parameter governing cadaver decomposition in soil. Forensic Sci Int. 2010;200:60–6.CrossRefPubMed
9.
Zurück zum Zitat Meyer J, Anderson B, Carter DO. Seasonal variation of carcass decomposition and gravesoil chemistry in a cold (Dfa) climate. J Forensic Sci. 2013;58:1175–82.CrossRefPubMed Meyer J, Anderson B, Carter DO. Seasonal variation of carcass decomposition and gravesoil chemistry in a cold (Dfa) climate. J Forensic Sci. 2013;58:1175–82.CrossRefPubMed
10.
Zurück zum Zitat Benbow ME, Lewis AJ, Tomberlin JK, Pechal JL. Seasonal necrophagous insect community assembly during vertebrate carrion decomposition. J Med Entomol. 2013;50:440–50.CrossRefPubMed Benbow ME, Lewis AJ, Tomberlin JK, Pechal JL. Seasonal necrophagous insect community assembly during vertebrate carrion decomposition. J Med Entomol. 2013;50:440–50.CrossRefPubMed
11.
Zurück zum Zitat Peel MC, Finlayson BL, McMahon TA. Updated world map of the Köppen–Geiger climate classification. Hydrol Earth Syst Sci. 2007;11:1633–44.CrossRef Peel MC, Finlayson BL, McMahon TA. Updated world map of the Köppen–Geiger climate classification. Hydrol Earth Syst Sci. 2007;11:1633–44.CrossRef
12.
Zurück zum Zitat Caporaso JG, Kuczynski J, Stombaugh J, Bittinger K, Bushman FD, Costello EK, et al. QIIME allows analysis of high-throughput community sequencing data. Nat Methods. 2010;7:335–6.CrossRefPubMedCentralPubMed Caporaso JG, Kuczynski J, Stombaugh J, Bittinger K, Bushman FD, Costello EK, et al. QIIME allows analysis of high-throughput community sequencing data. Nat Methods. 2010;7:335–6.CrossRefPubMedCentralPubMed
13.
Zurück zum Zitat Putman RJ. Patterns of carbon dioxide evolution from decaying carrion. 1. Decomposition of small mammal carrion in temperate systems. Oikos. 1978;31:47–57.CrossRef Putman RJ. Patterns of carbon dioxide evolution from decaying carrion. 1. Decomposition of small mammal carrion in temperate systems. Oikos. 1978;31:47–57.CrossRef
14.
Zurück zum Zitat Putman RJ. Flow of energy and organic matter from a carcase during decomposition. 2. Decomposition of small mammal carrion in temperate systems. Oikos. 1978;31:58–68.CrossRef Putman RJ. Flow of energy and organic matter from a carcase during decomposition. 2. Decomposition of small mammal carrion in temperate systems. Oikos. 1978;31:58–68.CrossRef
15.
Zurück zum Zitat Lauber CL, Metcalf JL, Keepers K, Ackermann G, Carter DO, Knight R. Vertebrate decomposition is accelerated by soil microbes. Appl Environ Microbiol. 2014;80:4920–9.CrossRefPubMedCentralPubMed Lauber CL, Metcalf JL, Keepers K, Ackermann G, Carter DO, Knight R. Vertebrate decomposition is accelerated by soil microbes. Appl Environ Microbiol. 2014;80:4920–9.CrossRefPubMedCentralPubMed
16.
Zurück zum Zitat Evans WED. The chemistry of death. Springfield: Charles C. Thomas; 1963. Evans WED. The chemistry of death. Springfield: Charles C. Thomas; 1963.
17.
Zurück zum Zitat Fierer N, Ladau J, Clemente JC, Leff JW, Owens SM, Pollard KS, et al. Reconstructing the microbial diversity and function of pre-agricultural tallgrass prairie soils in the United States. Science. 2013;6158:621–4.CrossRef Fierer N, Ladau J, Clemente JC, Leff JW, Owens SM, Pollard KS, et al. Reconstructing the microbial diversity and function of pre-agricultural tallgrass prairie soils in the United States. Science. 2013;6158:621–4.CrossRef
18.
Zurück zum Zitat Bongers T, Ferris H. Nematode community structure as a bioindicator in environmental sampling. Trends Ecol Evol. 1999;14:224–8.CrossRefPubMed Bongers T, Ferris H. Nematode community structure as a bioindicator in environmental sampling. Trends Ecol Evol. 1999;14:224–8.CrossRefPubMed
19.
Zurück zum Zitat Tibbett M, Carter DO. Mushrooms and taphonomy: the fungi that mark woodland graves. Mycologist. 2003;17:20–4.CrossRef Tibbett M, Carter DO. Mushrooms and taphonomy: the fungi that mark woodland graves. Mycologist. 2003;17:20–4.CrossRef
20.
Zurück zum Zitat Sagara N, Yamanaka T, Tibbett M. Soil fungi associated with graves and latrines: toward a forensic mycology. In: Tibbett M, Carter DO, editors. Soil analysis in forensic taphonomy: chemical and biological effects of buried human remains. Boca Raton: CRC Press; 2008. p. 67–108.CrossRef Sagara N, Yamanaka T, Tibbett M. Soil fungi associated with graves and latrines: toward a forensic mycology. In: Tibbett M, Carter DO, editors. Soil analysis in forensic taphonomy: chemical and biological effects of buried human remains. Boca Raton: CRC Press; 2008. p. 67–108.CrossRef
Metadaten
Titel
Seasonal variation of postmortem microbial communities
verfasst von
David O. Carter
Jessica L. Metcalf
Alexander Bibat
Rob Knight
Publikationsdatum
01.06.2015
Verlag
Springer US
Erschienen in
Forensic Science, Medicine and Pathology / Ausgabe 2/2015
Print ISSN: 1547-769X
Elektronische ISSN: 1556-2891
DOI
https://doi.org/10.1007/s12024-015-9667-7

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