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03.04.2018 | Gynecologic Endocrinology and Reproductive Medicine

Serum levels of Pentraxin 3 differ significantly at the time of blastocyst transfer depending on implantation success: a pilot study

verfasst von: Alexander Freis, Kyra Von Horn, Tamara Göggl, Stephanie Hecht, Sabine Roesner, Thomas Strowitzki, Ariane Germeyer

Erschienen in: Archives of Gynecology and Obstetrics | Ausgabe 6/2018

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Abstract

Objective

Many approaches try to identify the underlying molecular mechanisms to detect new potential biomarkers for successful artificial reproductive therapies. One factor has been described as a possible regulator of inflammation during implantation: Pentraxin 3 (PTX3), which seems to be essential for female fertility on one hand, but whose overexpression has been described in many obstetric complications based on abnormal placentation on the other hand. Therefore, we investigated if serum levels of PTX3 at the time of embryo transfer differ between women with an ongoing pregnancy compared to those without implantation.

Methods/design

During in vitro fertilization cycles of 51 patients, PTX3 levels at the time of embryo transfer were compared between patients without implantation (n = 26) and those with ongoing pregnancy (n = 25) using an enzyme-linked immunosorbent assay. Statistical analysis was performed using the Kolmogorov–Smirnov test, Fisher's exact test and Student’s t test

Results

No significant differences were found concerning possible confounders (patients age, smoking pattern, embryo quality, number of embryos transferred and prior IVF attempts). Patients without implantation presented with significantly higher serum levels of PTX3 at the time of embryo transfer compared to women who became pregnant (0.781 ± 0.074 ng/ml vs. 0.578 ± 0.055 ng/ml, p < 0.05).

Conclusions

PTX3 could present as a possible biomarker for ART success. The main limitation of this pilot study is its small sample size that needs validation with a larger study population.

Szekeres-Bartho J (2015) Successful implantation from the embryonic aspect. Am J Reprod Immunol 75:382–387. https://doi.org/10.1111/aji.12448 CrossRefPubMed

Popovici RM, Betzler NK, Krause MS et al (2006) Gene expression profiling of human endometrial-trophoblast interaction in a coculture model. Endocrinology 147:5662–5675. https://doi.org/10.1210/en.2006-0916 CrossRefPubMed

Schjenken JE, Zhang B, Chan HY et al (2016) miRNA regulation of immune tolerance in early pregnancy. Am J Reprod Immunol 75:272–280. https://doi.org/10.1111/aji.12490 CrossRefPubMed

Garlanda C, Maina V, Martinez de la Torre Y et al (2008) Inflammatory reaction and implantation: the new entries PTX3 and D6. Placenta 29:129–134. https://doi.org/10.1016/j.placenta.2008.06.008 CrossRefPubMed

Doni A, Garlanda C, Mantovani A (2016) Innate immunity, hemostasis and matrix remodeling: PTX3 as a link. Semin Immunol 28:570–577. https://doi.org/10.1016/j.smim.2016.10.012 CrossRefPubMedPubMedCentral

Kume N, Mitsuoka H, Hayashida K, Tanaka M (2011) Pentraxin 3 as a biomarker for acute coronary syndrome: comparison with biomarkers for cardiac damage. J Cardiol 58:38–45. https://doi.org/10.1016/j.jjcc.2011.03.006 CrossRefPubMed

Nerkiz P, Doganer YC, Aydogan U et al (2015) Serum pentraxin-3 level in patients who underwent coronary angiography and relationship with coronary atherosclerosis. Med Princ Pract 24:369–375. https://doi.org/10.1159/000381879 CrossRefPubMedPubMedCentral

Daigo K, Inforzato A, Barajon I et al (2016) Pentraxins in the activation and regulation of innate immunity. Immunol Rev 274:202–217. https://doi.org/10.1111/imr.12476 CrossRefPubMedPubMedCentral

Popovici RM, Krause MS, Jauckus J et al (2008) The long pentraxin PTX3 in human endometrium: regulation by steroids and trophoblast products. Endocrinology 149:1136–1143. https://doi.org/10.1210/en.2007-1302 CrossRefPubMed

10.

Salustri A (2004) PTX3 plays a key role in the organization of the cumulus oophorus extracellular matrix and in in vivo fertilization. Development 131:1577–1586. https://doi.org/10.1242/dev.01056 CrossRefPubMed

11.

Garlanda C, Bottazzi B, Bastone A, Mantovani A (2005) Pentraxins at the crossroads between innate immunity, inflammation, matrix deposition, and female fertility. Annu Rev Immunol 23:337–366. https://doi.org/10.1146/annurev.immunol.23.021704.115756 CrossRefPubMed

12.

Tranguch S, Chakrabarty A, Guo Y et al (2007) Maternal pentraxin 3 deficiency compromises implantation in mice. Biol Reprod 77:425–432. https://doi.org/10.1095/biolreprod.107.062414 CrossRefPubMed

13.

Zhang X, Jafari N, Barnes RB et al (2005) Studies of gene expression in human cumulus cells indicate pentraxin 3 as a possible marker for oocyte quality. Fertil Steril 83(Suppl 1):1169–1179. https://doi.org/10.1016/j.fertnstert.2004.11.030 CrossRefPubMed

14.

Nakashima A, Aoki A, Kusabiraki T et al (2017) Role of autophagy in oocytogenesis, embryogenesis, implantation, and pathophysiology of pre-eclampsia. J Obstet Gynaecol Res 43:633–643. https://doi.org/10.1111/jog.13292 CrossRefPubMed

15.

Cetin I, Cozzi V, Pasqualini F et al (2006) Elevated maternal levels of the long pentraxin 3 (PTX3) in preeclampsia and intrauterine growth restriction. Am J Obstet Gynecol 194:1347–1353. https://doi.org/10.1016/j.ajog.2005.11.018 CrossRefPubMed

16.

Ibrahim MI, Harb HM, Ellaithy MI et al (2012) First trimester assessment of Pentraxin-3 levels in women with primary unexplained recurrent pregnancy loss. Eur J Obstet Gynecol 165:37–41. https://doi.org/10.1016/j.ejogrb.2012.07.016 CrossRef

17.

Fornai F, Carrizzo A, Forte M et al (2016) The inflammatory protein pentraxin 3 in cardiovascular disease. Immun Ageing. https://doi.org/10.1186/s12979-016-0080-1 PubMedPubMedCentral

18.

Monach PA (2014) Biomarkers in vasculitis. Curr Opin Rheumatol 26:24–30. https://doi.org/10.1097/BOR.0000000000000009 CrossRefPubMedPubMedCentral

19.

Ortega-Hernandez OD, Bassi N, Shoenfeld Y, Anaya JM (2009) The long pentraxin 3 and its role in autoimmunity. YSARH 39:38–54. https://doi.org/10.1016/j.semarthrit.2008.03.006

20.

Agrawal A, Singh PP, Bottazzi B et al (2009) Pattern recognition by pentraxins. Adv Exp Med Biol 653:98–116CrossRefPubMedPubMedCentral

21.

Robertson SA, Jin M, Yu D et al (2016) Corticosteroid therapy in assisted reproduction—immune suppression is a faulty premise. Hum Reprod 31:2164–2173. https://doi.org/10.1093/humrep/dew186 CrossRefPubMed

22.

Sanchez-Lopez JA, Caballero I, Montazeri M et al (2014) Local activation of uterine toll-like receptor 2 and 2/6 decreases embryo implantation and affects uterine receptivity in mice. Biol Reprod 90:1–13. https://doi.org/10.1095/biolreprod.113.115253 CrossRef

23.

Ruíz-Alonso M, Blesa D, Díaz-Gimeno P et al (2013) The endometrial receptivity array for diagnosis and personalized embryo transfer as a treatment for patients with repeated implantation failure. Fertil Steril 100:818–824. https://doi.org/10.1016/j.fertnstert.2013.05.004 CrossRefPubMed

24.

Muller B, Peri G, Doni A et al (2001) Circulating levels of the long pentraxin PTX3 correlate with severity of infection in critically ill patients. Crit Care Med 29:1404–1407CrossRefPubMed

25.

Peter Durairaj RR, Aberkane A, Polanski L et al (2017) Deregulation of the endometrial stromal cell secretome precedes embryo implantation failure. Mol Hum Reprod. https://doi.org/10.1093/molehr/gax023

26.

Saito S, Nakashima A, Shima T, Ito M (2010) Th1/Th2/Th17 and regulatory T-cell paradigm in pregnancy. Am J Reprod Immunol 63:601–610. https://doi.org/10.1111/j.1600-0897.2010.00852.x CrossRefPubMed

27.

Zhang J, Koussih L, Shan L et al (2015) TNF up-regulates pentraxin 3 expression in human airway smooth muscle cells via JNK and ERK1/2 MAPK pathways. Allergy Asthma Clin Immunol. https://doi.org/10.1186/s13223-015-0104-y PubMedPubMedCentral

28.

Han B, Mura M, Andrade CF et al (2005) TNF-induced long pentraxin PTX3 expression in human lung epithelial cells via JNK. J Immunol 175:8303–8311. https://doi.org/10.4049/jimmunol.175.12.8303 CrossRefPubMed

29.

Bouvier S, Paulmyer-Lacroix O, Molinari N et al (2017) Soluble CD146, an innovative and non-invasive biomarker of embryo selection for in vitro fertilization. PLoS ONE 12(3):e0173724. https://doi.org/10.1371/journal.pone.0173724 CrossRefPubMedPubMedCentral

30.

Altmae S, Esteban FJ, Stavreus-Evers A et al (2013) Guidelines for the design, analysis and interpretation of “omics” data: focus on human endometrium. Hum Reprod Update 20:12–28. https://doi.org/10.1093/humupd/dmt048 CrossRefPubMedPubMedCentral

31.

Ishihara O, Araki R, Kuwahara A et al (2014) Impact of frozen-thawed single-blastocyst transfer on maternal and neonatal outcome: an analysis of 277,042 single-embryo transfer cycles from 2008 to 2010 in Japan. Fertil Steril 101:128–133. https://doi.org/10.1016/j.fertnstert.2013.09.025 CrossRefPubMed

32.

Roque M (2014) Freeze-all policy: is it time for that? J Assist Reprod Genet 32:171–176. https://doi.org/10.1007/s10815-014-0391-0 CrossRefPubMedPubMedCentral

Titel: Serum levels of Pentraxin 3 differ significantly at the time of blastocyst transfer depending on implantation success: a pilot study
verfasst von: Alexander Freis
Kyra Von Horn
Tamara Göggl
Stephanie Hecht
Sabine Roesner
Thomas Strowitzki
Ariane Germeyer
Publikationsdatum: 03.04.2018
Verlag: Springer Berlin Heidelberg
Erschienen in: Archives of Gynecology and Obstetrics / Ausgabe 6/2018
Print ISSN: 0932-0067
Elektronische ISSN: 1432-0711
DOI: https://doi.org/10.1007/s00404-018-4769-6

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Serum levels of Pentraxin 3 differ significantly at the time of blastocyst transfer depending on implantation success: a pilot study