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Journal of the Association for Research in Otolaryngology

Erschienen in:

08.09.2023 | Research Article

Sheep as a Large-Animal Model for Otology Research: Temporal Bone Extraction and Transmastoid Facial Recess Surgical Approach

verfasst von: Nicholas A. Waring, Alexander Chern, Brandon J. Vilarello, Jeffrey H. Lang, Elizabeth S. Olson, Hideko Heidi Nakajima

Erschienen in: Journal of the Association for Research in Otolaryngology | Ausgabe 5/2023

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Abstract

Purpose

Sheep are used as a large-animal model for otology research and can be used to study implantable hearing devices. However, a method for temporal bone extraction in sheep, which enables various experiments, has not been described, and literature on middle ear access is limited. We describe a method for temporal bone extraction and an extended facial recess surgical approach to the middle ear in sheep.

Methods

Ten temporal bones from five Hampshire sheep head cadavers were extracted using an oscillating saw. After craniotomy and removal of the brain, a coronal cut was made at the posterior aspect of the orbit followed by a midsagittal cut of the occipital bone and disarticulation of the atlanto-occipital joint. Temporal bones were surgically prepared with an extended facial recess approach. Micro-CT scans of each temporal bone were obtained, and anatomic dimensions were measured.

Results

Temporal bone extraction was successful in 10/10 temporal bones. Extended facial recess approach exposed the malleus, incus, stapes, and round window while preserving the facial nerve, with the following surgical considerations: minimally pneumatized mastoid; tegmen (superior limit of mastoid cavity) is low-lying and sits below temporal artery; chorda tympani sacrificed to optimize middle ear exposure; incus buttress does not obscure view of middle ear. Distance between the superior aspect of external auditory canal and tegmen was 2.7 (SD 0.9) mm.

Conclusion

We identified anatomic landmarks for temporal bone extraction and describe an extended facial recess approach in sheep that exposes the ossicles and round window. This approach is feasible for studying implantable hearing devices.

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Gurr A, Pearson MD, Dazert S (2011) Lambs’ temporal bone anatomy under didactic aspects. Braz J Otorhinolaryngol 77(1):51–57. https://doi.org/10.1590/s1808-86942011000100009CrossRefPubMed

Soares HB, Lavinsky L (2011) Histology of sheep temporal bone. Braz J Otorhinolaryngol 77(3):285–292. https://doi.org/10.1590/s1808-86942011000300003CrossRefPubMed

Seibel VA, Lavinsky L, De Oliveira JA (2006) Morphometric study of the external and middle ear anatomy in sheep: a possible model for ear experiments. Clin Anat 19(6):503–509. https://doi.org/10.1002/ca.20218CrossRefPubMed

Seibel VA, Lavinsky L, Irion K (2006) CT-Scan sheep and human inner ear morphometric comparison. Braz J Otorhinolaryngol 72(3):370–376. https://doi.org/10.1016/s1808-8694(15)30971-xCrossRefPubMed

Schnabl J, Glueckert R, Feuchtner G, Recheis W, Potrusil T, Kuhn V, Wolf-Magele A, Riechelmann H, Sprinzl GM (2012) Sheep as a large animal model for middle and inner ear implantable hearing devices: a feasibility study in cadavers. Otol Neurotol 33(3):481–489. https://doi.org/10.1097/MAO.0b013e318248ee3aCrossRefPubMed

Han S, Suzuki-Kerr H, Suwantika M, Telang RS, Gerneke DA, Anekal PV, Bird P, Vlajkovic SM, Thorne PR (2021) Characterization of the sheep round window membrane. J Assoc Res Otolaryngol 22(1):1–17. https://doi.org/10.1007/s10162-020-00778-9CrossRefPubMed

Kerneis S, Escoffre J-M, Galvin JJ, Bouakaz A, Presset A, Alix C, Oujagir E, Lefèvre A, Emond P, Blasco H, Bakhos D (2023) Sonoporation of the round window membrane on a sheep model: a safety study. Pharmaceutics 15(2). https://doi.org/10.3390/pharmaceutics15020442

Ames DR, Arehart LA (1972) Physiological response of lambs to auditory stimuli. J Anim Sci 34(6):994–998. https://doi.org/10.2527/jas1972.346994xCrossRefPubMed

Peus D, Dobrev I, Prochazka L, Thoele K, Dalbert A, Boss A, Newcomb N, Probst R, Roosli C, Sim JH, Huber A, Pfiffner F (2017) Sheep as a large animal ear model: middle-ear ossicular velocities and intracochlear sound pressure. Hear Res 351:88–97. https://doi.org/10.1016/j.heares.2017.06.002CrossRefPubMed

10.

Peus D, Dobrev I, Pfiffner F, Sim JH (2020) Comparison of sheep and human middle-ear ossicles: anatomy and inertial properties. J Comp Physiol A Neuroethol Sens Neural Behav Physiol 206(5):683–700. https://doi.org/10.1007/s00359-020-01430-wCrossRefPubMedCentralPubMed

11.

McFadden D, Pasanen EG, Valero MD, Roberts EK, Lee TM (2008) Dissociation between distortion-product and click-evoked otoacoustic emissions in sheep (Ovis aries). J Acoust Soc Am 124(6):3730–3738. https://doi.org/10.1121/1.2982402CrossRefPubMedCentralPubMed

12.

McFadden D, Pasanen EG, Valero MD, Roberts EK, Lee TM (2009) Effect of prenatal androgens on click-evoked otoacoustic emissions in male and female sheep (Ovis aries). Horm Behav 55(1):98–105. https://doi.org/10.1016/j.yhbeh.2008.08.013CrossRefPubMed

13.

Maia FC, Lavinsky L, Mollerke RO, Duarte ME, Pereira DP, Maia JE (2008) Distortion product otoacoustic emissions in sheep before and after hyperinsulinemia induction. Braz J Otorhinolaryngol 74(2):181–187. https://doi.org/10.1016/s1808-8694(15)31086-7CrossRefPubMed

14.

Zuma e Maia FC, Lavinsky L (2006) Distortion product otoacoustic emissions in an animal model of induced hyperinsulinemia. Int Tinnitus J 12(2):133–9

15.

Angeli RD, Lavinsky L, Dolganov A (2009) Alterations in cochlear function during induced acute hyperinsulinemia in an animal model. Braz J Otorhinolaryngol 75(5):760–764. https://doi.org/10.1016/s1808-8694(15)30530-9CrossRefPubMed

16.

Boullaud L, Blasco H, Caillaud E, Emond P, Bakhos D (2022) Immediate-early modifications to the metabolomic profile of the perilymph following an acoustic trauma in a sheep model. J Clin Med 11(16). https://doi.org/10.3390/jcm11164668

17.

Pohl F, Paasche G, Lenarz T, Schuon R (2017) Tympanometric measurements in conscious sheep - a diagnostic tool for pre-clinical middle ear implant studies. Int J Audiol 56(1):53–61. https://doi.org/10.1080/14992027.2016.1227480CrossRefPubMed

18.

Gocer C, Eryilmaz A, Genc U, Dagli M, Karabulut H, Iriz A (2007) An alternative model for stapedectomy training in residency program: sheep cadaver ear. Eur Arch Otorhinolaryngol 264(12):1409–1412. https://doi.org/10.1007/s00405-007-0437-3CrossRefPubMed

19.

Cordero A, del mar Medina M, Alonso A, Labatut T (2011) Stapedectomy in sheep: an animal model for surgical training. Otol Neurotol 32(5):742–7. https://doi.org/10.1097/MAO.0b013e31821ddbc2

20.

Cordero A, Benitez S, Reyes P, Vaca M, Polo R, Perez C, Alonso A, Cobeta I (2015) Ovine ear model for fully endoscopic stapedectomy training. Eur Arch Otorhinolaryngol 272(9):2167–2174. https://doi.org/10.1007/s00405-014-3114-3CrossRefPubMed

21.

Anschuetz L, Bonali M, Ghirelli M, Mattioli F, Villari D, Caversaccio M, Presutti L (2017) An ovine model for exclusive endoscopic ear surgery. JAMA Otolaryngol Head Neck Surg 143(3):247–252. https://doi.org/10.1001/jamaoto.2016.3315CrossRefPubMed

22.

Beckmann S, Yacoub A, Fernandez IJ, Niederhauser L, Fermi M, Caversaccio M, Bonali M, Anschuetz L (2021) Exclusive endoscopic laser-stapedotomy: feasibility of an ovine training model. Otol Neurotol 42(7):994–1000. https://doi.org/10.1097/MAO.0000000000003168CrossRefPubMed

23.

Shrivastava T, Khan MM, Parab SR (2022) Learning Curve of Two Handed Endoscopic Ear Surgery on Sheep Temporal Bone: A Fellow’s Perspective. Indian J Otolaryngol Head Neck Surg 74(Suppl 1):550–558. https://doi.org/10.1007/s12070-021-02388-0CrossRefPubMed

24.

Okhovat S, Milner TD, Iyer A (2019) Feasibility of ovine and synthetic temporal bone models for simulation training in endoscopic ear surgery. J Laryngol Otol 133(11):966–973. https://doi.org/10.1017/S0022215119002135CrossRefPubMed

25.

Mantokoudis G, Huth ME, Weisstanner C, Friedrich HM, Nauer C, Candreia C, Caversaccio MD, Senn P (2016) Lamb temporal bone as a surgical training model of round window cochlear implant electrode insertion. Otol Neurotol 37(1):52–56. https://doi.org/10.1097/MAO.0000000000000921CrossRefPubMed

26.

Trinh TT, Cohen C, Boullaud L, Cottier JP, Bakhos D (2021) Sheep as a large animal model for cochlear implantation. Braz J Otorhinolaryngol. https://doi.org/10.1016/j.bjorl.2021.02.014CrossRefPubMedCentralPubMed

27.

Weisstanner C, Mantokoudis G, Huth M, Verma RK, Nauer C, Senn P, Caversaccio MD, Wagner F (2015) Radiation dose reduction in postoperative computed position control of cochlear implant electrodes in lambs - an experimental study. Int J Pediatr Otorhinolaryngol 79(12):2348–2354. https://doi.org/10.1016/j.ijporl.2015.10.040CrossRefPubMed

28.

Lavinsky L, Goycoolea M, Gananca MM, Zwetsch Y (1999) Surgical treatment of vertigo by utriculostomy: an experimental study in sheep. Acta Otolaryngol 119(5):522–527. https://doi.org/10.1080/00016489950180739CrossRefPubMed

29.

Neudert M, Beleites T, Ney M, Kluge A, Lasurashvili N, Bornitz M, Scharnweber D, Zahnert T (2010) Osseointegration of titanium prostheses on the stapes footplate. J Assoc Res Otolaryngol 11(2):161–171. https://doi.org/10.1007/s10162-009-0202-yCrossRefPubMedCentralPubMed

30.

Piu F, Wang X, Fernandez R, Dellamary L, Harrop A, Ye Q, Sweet J, Tapp R, Dolan DF, Altschuler RA, Lichter J, LeBel C (2011) OTO-104: a sustained-release dexamethasone hydrogel for the treatment of otic disorders. Otol Neurotol 32(1):171–179. https://doi.org/10.1097/MAO.0b013e3182009d29CrossRefPubMed

31.

Wang X, Fernandez R, Dellamary L, Harrop A, Ye Q, Lichter J, Lau D, Lebel C, Piu F (2011) Pharmacokinetics of dexamethasone solution following intratympanic injection in guinea pig and sheep. Audiol Neurootol 16(4):233–241. https://doi.org/10.1159/000320611CrossRefPubMed

32.

Larsson A, Andersson M, Wigren S, Pivodic A, Flynn M, Nannmark U (2015) Soft tissue integration of hydroxyapatite-coated abutments for bone conduction implants. Clin Implant Dent Relat Res 17(Suppl 2):e730–e735. https://doi.org/10.1111/cid.12304CrossRefPubMed

33.

Larsson A, Wigren S, Andersson M, Ekeroth G, Flynn M, Nannmark U (2012) Histologic evaluation of soft tissue integration of experimental abutments for bone anchored hearing implants using surgery without soft tissue reduction. Otol Neurotol 33(8):1445–1451. https://doi.org/10.1097/MAO.0b013e318268d4e0CrossRefPubMed

34.

Taghavi H, Hakansson B, Eeg-Olofsson M, Johansson CB, Tjellstrom A, Reinfeldt S, Bergqvist T, Olsson J (2013) A vibration investigation of a flat surface contact to skull bone for direct bone conduction transmission in sheep skulls in vivo. Otol Neurotol 34(4):690–698. https://doi.org/10.1097/MAO.0b013e3182877aeeCrossRefPubMed

35.

Kaufmann CR, Tejani VD, Fredericks DC, Henslee AM, Sun DQ, Abbas PJ, Hansen MR (2020) Pilot evaluation of sheep as in vivo model for cochlear implantation. Otol Neurotol 41(5):596–604. https://doi.org/10.1097/MAO.0000000000002587CrossRefPubMed

36.

Anso J, Stahl C, Gerber N, Williamson T, Gavaghan K, Rosler KM, Caversaccio MD, Weber S, Bell B (2014) Feasibility of using EMG for early detection of the facial nerve during robotic direct cochlear access. Otol Neurotol 35(3):545–554. https://doi.org/10.1097/MAO.0000000000000187CrossRefPubMed

37.

Wyss Balmer T, Anso J, Muntane E, Gavaghan K, Weber S, Stahel A, Buchler P (2017) In-vivo electrical impedance measurement in mastoid bone. Ann Biomed Eng 45(4):1122–1132. https://doi.org/10.1007/s10439-016-1758-4CrossRefPubMed

38.

Feldmann A, Anso J, Bell B, Williamson T, Gavaghan K, Gerber N, Rohrbach H, Weber S, Zysset P (2016) Temperature prediction model for bone drilling based on density distribution and in vivo experiments for minimally invasive robotic cochlear implantation. Ann Biomed Eng 44(5):1576–1586. https://doi.org/10.1007/s10439-015-1450-0CrossRefPubMed

39.

Anso J, Balmer TW, Jegge Y, Kalvoy H, Bell BJ, Dur C, Calvo EM, Williamson TM, Gerber N, Ferrario D, Forterre F, Buchler P, Stahel A, Caversaccio MD, Weber S, Gavaghan KA (2019) Electrical impedance to assess facial nerve proximity during robotic cochlear implantation. IEEE Trans Biomed Eng 66(1):237–245. https://doi.org/10.1109/TBME.2018.2830303CrossRefPubMed

40.

Henslee AM, Kaufmann CR, Andrick MD, Reineke PT, Tejani VD, Hansen MR (2022) Development and characterization of an electrocochleography-guided robotics-assisted cochlear implant array insertion system. Otolaryngol Head Neck Surg 167(2):334–340. https://doi.org/10.1177/01945998211049210CrossRefPubMed

41.

Djinovic Z, Pavelka R, Tomic M, Sprinzl G, Plenk H, Losert U, Bergmeister H, Plasenzotti R (2018) In-vitro and in-vivo measurement of the animal’s middle ear acoustical response by partially implantable fiber-optic sensing system. Biosens Bioelectron 103:176–181. https://doi.org/10.1016/j.bios.2017.12.015CrossRefPubMed

42.

Pfiffner F, Prochazka L, Dobrev I, Klein K, Sulser P, Péus D, Sim JH, Dalbert A, Röösli C, Obrist D, Huber A (2018) Proof of concept for an intracochlear acoustic receiver for use in acute large animal experiments. Sensors (Basel) 18(10). https://doi.org/10.3390/s18103565

43.

Fedorov A, Beichel R, Kalpathy-Cramer J, Finet J, Fillion-Robin JC, Pujol S, Bauer C, Jennings D, Fennessy F, Sonka M, Buatti J, Aylward S, Miller JV, Pieper S, Kikinis R (2012) 3D Slicer as an image computing platform for the Quantitative Imaging Network. Magn Reson Imaging 30(9):1323–1341. https://doi.org/10.1016/j.mri.2012.05.001CrossRefPubMedCentralPubMed

44.

Dinh C, Szczupak M, Moon S, Angeli S, Eshraghi A, Telischi FF (2015) Human temporal bone removal: the skull base block method. J Neurol Surg B Skull Base 76(4):278–280. https://doi.org/10.1055/s-0034-1543972CrossRefPubMedCentralPubMed

45.

Makki FM, Amoodi HA, van Wijhe RG, Bance M (2011) Anatomic analysis of the mastoid tegmen: slopes and tegmen shape variances. Otol Neurotol 32(4):581–588. https://doi.org/10.1097/MAO.0b013e31820e75f7CrossRefPubMed

46.

Yildiz E, Gerlitz M, Gadenstaetter AJ, Landegger LD, Nieratschker M, Schum D, Schmied M, Haase A, Kanz F, Kramer AM, Glueckert R, Staecker H, Honeder C, Arnoldner C (2022) Single-incision cochlear implantation and hearing evaluation in piglets and minipigs. Hear Res 426:108644. https://doi.org/10.1016/j.heares.2022.108644

47.

Fermi M, Chiari F, Mattioli F, Bonali M, Molinari G, Alicandri-Ciufelli M, Anschuetz L, Fernandez IJ, Presutti L (2022) Surgical training on ex vivo ovine model in otolaryngology head and neck surgery: a comprehensive review. Int J Environ Res Public Health 19(6). https://doi.org/10.3390/ijerph19063657

Titel: Sheep as a Large-Animal Model for Otology Research: Temporal Bone Extraction and Transmastoid Facial Recess Surgical Approach
verfasst von: Nicholas A. Waring
Alexander Chern
Brandon J. Vilarello
Jeffrey H. Lang
Elizabeth S. Olson
Hideko Heidi Nakajima
Publikationsdatum: 08.09.2023
Verlag: Springer US
Erschienen in: Journal of the Association for Research in Otolaryngology / Ausgabe 5/2023
Print ISSN: 1525-3961
Elektronische ISSN: 1438-7573
DOI: https://doi.org/10.1007/s10162-023-00907-0

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Sheep as a Large-Animal Model for Otology Research: Temporal Bone Extraction and Transmastoid Facial Recess Surgical Approach

Abstract

Purpose

Methods

Results

Conclusion

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Abstract

Purpose

Methods

Results

Conclusion

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