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01.07.2010 | Original Article

SIRT1, AMP-activated protein kinase phosphorylation and downstream kinases in response to a single bout of sprint exercise: influence of glucose ingestion

verfasst von: Borja Guerra, Amelia Guadalupe-Grau, Teresa Fuentes, Jesús Gustavo Ponce-González, David Morales-Alamo, Hugo Olmedillas, José Guillén-Salgado, Alfredo Santana, José A. L. Calbet

Erschienen in: European Journal of Applied Physiology | Ausgabe 4/2010

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Abstract

This study was designed to examine potential in vivo mechanisms of AMP-activated protein kinase (AMPK) phosphorylation inhibition and its downstream signaling consequences during the recovery period after a single bout of sprint exercise. Sprint exercise induces Thr¹⁷²-AMPK phosphorylation and increased PGC-1α mRNA, by an unknown mechanism. Muscle biopsies were obtained in 15 young healthy men in response to a 30-s sprint exercise (Wingate test) randomly distributed into two groups: the fasting (n = 7, C) and the glucose group (n = 8, G), who ingested 75 g of glucose 1 h before exercising to inhibit AMPKα phosphorylation. Exercise elicited different patterns of Ser²²¹-ACCβ, Ser⁴⁷³-Akt and Thr⁶⁴²-AS160 phosphorylation, during the recovery period after glucose ingestion. Thirty minutes after the control sprint, Ser⁴⁸⁵-AMPKα1/Ser⁴⁹¹-AMPKα2 phosphorylation was reduced by 33% coinciding with increased Thr¹⁷²-AMPKα phosphorylation (both, P < 0.05). Glucose abolished the 30-min Thr¹⁷²-AMPKα phosphorylation. Ser²²¹-ACCβ phosphorylation was elevated immediately following and 30 min after exercise in C and G, implying a dissociation between Thr¹⁷²-AMPKα and Ser²²¹-ACCβ phosphorylation. Two hours after the sprint, PGC-1α protein expression remained unchanged while SIRT1 (its upstream deacetylase) was increased. Glucose ingestion abolished the SIRT1 response without any significant effect on PGC-1α protein expression. In conclusion, glucose ingestion prior to a sprint exercise profoundly affects Thr¹⁷²-AMPKα phosphorylation and its downstream signaling during the recovery period.

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Titel: SIRT1, AMP-activated protein kinase phosphorylation and downstream kinases in response to a single bout of sprint exercise: influence of glucose ingestion
verfasst von: Borja Guerra
Amelia Guadalupe-Grau
Teresa Fuentes
Jesús Gustavo Ponce-González
David Morales-Alamo
Hugo Olmedillas
José Guillén-Salgado
Alfredo Santana
José A. L. Calbet
Publikationsdatum: 01.07.2010
Verlag: Springer-Verlag
Erschienen in: European Journal of Applied Physiology / Ausgabe 4/2010
Print ISSN: 1439-6319
Elektronische ISSN: 1439-6327
DOI: https://doi.org/10.1007/s00421-010-1413-y

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