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European Journal of Nutrition

Erschienen in:

01.08.2009 | Original Contribution

Site-related white adipose tissue lipid-handling response to oleoyl-estrone treatment in overweight male rats

verfasst von: María del Mar Romero, José Antonio Fernández-López, Montserrat Esteve, Marià Alemany

Erschienen in: European Journal of Nutrition | Ausgabe 5/2009

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Abstract

Background

Oleoyl-estrone (OE) decreases energy intake while maintaining glucose homeostasis, and energy expenditure at the expense of body fat. White adipose tissue (WAT) depots behave differently under starvation, postprandial state and pharmacologically induced lipolysis.

Aim of the study

To understand the mechanism of massive lipid loss from WAT elicited by OE treatment.

Methods

We used overweight male rats. Rats receiving OE (10 nmol/g) gavages were compared with controls and a pair-fed group. Whole fat pads from the mesenteric, retroperitoneal, epididymal and inguinal subcutaneous sites were excised and analyzed for lipid, DNA, mRNA and the expression of lipogenic, fatty acid transporters and lipase genes.

Results

In OE and pair-fed rats, WAT weights decreased, with the limited loss of cells. Patterns of gene expression in most WAT sites were similar for OE and PF, suggesting a shared mechanism of fat mobilization, but in mesenteric WAT, PF increased lipogenic and fatty acid transporter gene expressions. However, OE inhibited lipogenic expressions more deeply than PF.

Conclusions

White adipose tissue sites showed different expression patterns, hinting at relatively specialized functions in fat storage; thus, single site analyses cannot be extrapolated to whole WAT. Differences between mesenteric and the other sites suggest that ‘visceral fat’ should be reserved for this site only, and not applied to other abdominal fat depots (epididymal, retroperitoneal).

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Titel: Site-related white adipose tissue lipid-handling response to oleoyl-estrone treatment in overweight male rats
verfasst von: María del Mar Romero
José Antonio Fernández-López
Montserrat Esteve
Marià Alemany
Publikationsdatum: 01.08.2009
Verlag: D. Steinkopff-Verlag
Erschienen in: European Journal of Nutrition / Ausgabe 5/2009
Print ISSN: 1436-6207
Elektronische ISSN: 1436-6215
DOI: https://doi.org/10.1007/s00394-009-0013-2

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Abstract

Background

Aim of the study

Methods

Results

Conclusions

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