nach oben

Digestive Diseases and Sciences

Erschienen in:

01.06.2009 | Original Article

Small-Intestinal Manifestations of Dextran Sulfate Sodium Consumption in Rats and Assessment of the Effects of Lactobacillus fermentum BR11

verfasst von: Mark S. Geier, Cassie L. Smith, Ross N. Butler, Gordon S. Howarth

Erschienen in: Digestive Diseases and Sciences | Ausgabe 6/2009

Einloggen, um Zugang zu erhalten

Abstract

The dextran sulfate sodium (DSS) colitis model has been utilized to screen for novel therapeutics for ulcerative colitis. Evidence suggests the small intestine may also be affected by DSS. We characterized the effects of DSS on the small intestine and assessed the potential for Lactobacillus fermentum BR11 to modify or normalize DSS-induced changes. Rats were allocated to three groups, Water + Vehicle, DSS + Vehicle, and DSS + L. fermentum BR11. BR11 was administered twice daily for 14 days. DSS (2%) was provided from days 7 to 14. Small-intestinal tissue was analyzed for sucrase activity, histology, and crypt cell proliferation. Increased ileum crypt depth and cell proliferation was observed in DSS-treated rats compared to controls (P < 0.05). BR11 normalized these parameters. While DSS predominantly induces colonic damage, minor morphological alterations were also detected in the distal small intestine. L. fermentum BR11 normalized these features.

Geier MS, Butler RN, Howarth GS. Inflammatory bowel disease: current insights into pathogenesis and new therapeutic options; probiotics, prebiotics and synbiotics. Int J Food Microbiol. 2007;115:1–11. doi:10.1016/j.ijfoodmicro.2006.10.006.PubMedCrossRef

Vowinkel T, Kalogeris TJ, Mori M, Krieglstein CF, Granger DN. Impact of dextran sulfate sodium load on the severity of inflammation in experimental colitis. Dig Dis Sci. 2004;49:556–564. doi:10.1023/B:DDAS.0000026298.72088.f7.PubMedCrossRef

Geier MS, Tenikoff D, Yazbeck R, McCaughan GW, Abbott CA, Howarth GS. Development and resolution of experimental colitis in mice with targeted deletion of dipeptidyl peptidase iv. J Cell Physiol. 2005;204:687–692. doi:10.1002/jcp.20333.PubMedCrossRef

Hendrickson BA, Gokhale R, Cho JH. Clinical aspects and pathophysiology of inflammatory bowel disease. Clin Microbiol Rev. 2002;15:79–94. doi:10.1128/CMR.15.1.79-94.2002.PubMedCrossRef

Tham DM, Whitin JC, Cohen HJ. Increased expression of extracellular glutathione peroxidase in mice with dextran sodium sulfate-induced experimental colitis. Pediatr Res. 2002;51:641–646.PubMed

Gaudier E, Michel C, Segain JP, Cherbut C, Hoebler C. The VSL#3 probiotic mixture modifies microflora but does not heal chronic dextran-sodium sulfate-induced colitis or reinforce the mucus barrier in mice. J Nutr. 2005;135:2753–2761.PubMed

Gaudio E, Taddei G, Vetuschi A, et al. Dextran sulfate sodium (DSS) colitis in rats: clinical, structural, and ultrastructural aspects. Dig Dis Sci. 1999;44:1458–1475. doi:10.1023/A:1026620322859.PubMedCrossRef

Kim EJ, Chung MY, Chung HJ, et al. Pharmacokinetics of 7-carboxymethyloxy-3′,4′,5-trimethoxy flavone (da-6034), a derivative of flavonoid, in mouse and rat models of chemically induced inflammatory bowel disease. J Pharm Pharmacol. 2006;58:27–35. doi:10.1211/jpp.58.1.0004.PubMedCrossRef

Ohtsuka Y, Sanderson IR. Dextran sulfate sodium-induced inflammation is enhanced by intestinal epithelial cell chemokine expression in mice. Pediatr Res. 2003;53:143–147.PubMedCrossRef

10.

Tanaka T, Kohno H, Suzuki R, et al. Dextran sodium sulfate strongly promotes colorectal carcinogenesis in apc(min/+) mice: Inflammatory stimuli by dextran sodium sulfate results in development of multiple colonic neoplasms. Int J Cancer. 2006;118:25–34. doi:10.1002/ijc.21282.PubMedCrossRef

11.

Takahashi S, Kawamura T, Kanda Y, et al. Multipotential acceptance of Peyer’s patches in the intestine for both thymus-derived t cells and extrathymic t cells in mice. Immunol Cell Biol. 2005;83:504–510. doi:10.1111/j.1440-1711.2005.01361.x.PubMedCrossRef

12.

Grabig A, Paclik D, Guzy C, et al. Escherichia coli strain Nissle 1917 ameliorates experimental colitis via toll-like receptor 2- and toll-like receptor 4-dependent pathways. Infect Immun. 2006;74:4075–4082. doi:10.1128/IAI.01449-05.PubMedCrossRef

13.

Kruis W, Fric P, Pokrotnieks J, et al. Maintaining remission of ulcerative colitis with the probiotic Escherichia coli Nissle 1917 is as effective as with standard mesalazine. Gut. 2004;53:1617–1623. doi:10.1136/gut.2003.037747.PubMedCrossRef

14.

Madsen KL, Doyle JS, Jewell LD, Tavernini MM, Fedorak RN. Lactobacillus species prevents colitis in interleukin 10 gene-deficient mice. Gastroenterology. 1999;116:1107–1114. doi:10.1016/S0016-5085(99)70013-2.PubMedCrossRef

15.

Dieleman LA, Goerres MS, Arends A, et al. Lactobacillus gg prevents recurrence of colitis in HLA-b27 transgenic rats after antibiotic treatment. Gut. 2003;52:370–376. doi:10.1136/gut.52.3.370.PubMedCrossRef

16.

Setoyama H, Imaoka A, Ishikawa H, Umesaki Y. Prevention of gut inflammation by bifidobacterium in dextran sulfate-treated gnotobiotic mice associated with bacteroides strains isolated from ulcerative colitis patients. Microbes Infect. 2003;5:115–122. doi:10.1016/S1286-4579(02)00080-1.PubMedCrossRef

17.

Shibolet O, Karmeli F, Eliakim R, et al. Variable response to probiotics in two models of experimental colitis in rats. Inflamm Bowel Dis. 2002;8:399–406. doi:10.1097/00054725-200211000-00004.PubMedCrossRef

18.

Bibiloni R, Fedorak RN, Tannock GW, et al. VSL#3 probiotic-mixture induces remission in patients with active ulcerative colitis. Am J Gastroenterol. 2005;100:1539–1546. doi:10.1111/j.1572-0241.2005.41794.x.PubMedCrossRef

19.

Herias MV, Koninkx JF, Vos JG, Huisin’t Veld JH, van Dijk JE. Probiotic effects of Lactobacillus casei on DSS-induced ulcerative colitis in mice. Int J Food Microbiol. 2005;103:143–155. doi:10.1016/j.ijfoodmicro.2004.11.032.PubMedCrossRef

20.

Osman N, Adawi D, Ahrne S, Jeppsson B, Molin G. Modulation of the effect of dextran sulfate sodium-induced acute colitis by the administration of different probiotic strains of lactobacillus and bifidobacterium. Dig Dis Sci. 2004;49:320–327. doi:10.1023/B:DDAS.0000017459.59088.43.PubMedCrossRef

21.

Rachmilewitz D, Katakura K, Karmeli F, et al. Toll-like receptor 9 signaling mediates the anti-inflammatory effects of probiotics in murine experimental colitis. Gastroenterology. 2004;126:520–528. doi:10.1053/j.gastro.2003.11.019.PubMedCrossRef

22.

Moon G, Myung SJ, Jeong JY, et al. Prophylactic effect of Lactobacillus GG in animal colitis and its effect on cytokine secretion and mucin gene expressions. Korean J Gastroenterol. 2004;43:234–245.PubMed

23.

Geier MS, Butler RN, Giffard PM, Howarth GS. Lactobacillus fermentum BR11, a potential new probiotic, alleviates symptoms of colitis induced by dextran sulfate sodium (DSS) in rats. Int J Food Microbiol. 2007;114:267–274. doi:10.1016/j.ijfoodmicro.2006.09.018.PubMedCrossRef

24.

Tomas FM, Knowles SE, Owens PC, et al. Effects of full-length and truncated insulin-like growth factor-i on nitrogen balance and muscle protein metabolism in nitrogen-restricted rats. J Endocrinol. 1991;128:97–105.PubMedCrossRef

25.

Pelton NC, Tivey DR, Howarth GS, Davidson GP, Butler RN. A novel breath test for the non-invasive assessment of small intestinal mucosal injury following methotrexate administration in the rat. Scand J Gastroenterol. 2004;39:1015–1016. doi:10.1080/00365520410003416.PubMedCrossRef

26.

Tooley KL, Howarth GS, Lymn KA, Lawrence A, Butler RN. Oral ingestion of streptococcus thermophilus diminishes severity of small intestinal mucositis in methotrexate treated rats. Cancer Biol Ther. 2006;5:593–600. doi:10.1158/1535-7163.MCT-05-0457.PubMedCrossRef

27.

Mauger CA, Butler RN, Geier MS, Tooley KL, Howarth GS. Probiotic effects on 5-fluorouracil-induced mucositis assessed by the sucrose breath test in rats. Dig Dis Sci. 2007;52:612–619. doi:10.1007/s10620-006-9464-y.PubMedCrossRef

28.

Torres DM, Tooley KL, Butler RN, Smith CL, Geier MS, Howarth GS. Lyprinol only partially improves indicators of small intestinal integrity in a rat model of 5-fluorouracil-induced mucositis. Cancer Biol Ther. 2008;7:295–302.PubMed

29.

Dahlqvist A. Assay of intestinal disaccharidases. Anal Biochem. 1968;22:99–107. doi:10.1016/0003-2697(68)90263-7.PubMedCrossRef

30.

Clarke JM, Pelton NC, Bajka BH, Howarth GS, Read LC, Butler RN. Use of the C-13-sucrose breath test to assess chemotherapy-induced small intestinal mucositis in the rat. Cancer Biol Ther. 2006;5:34–38.PubMedCrossRef

31.

Hong MY, Turner ND, Carroll RJ, Chapkin RS, Lupton JR. Differential response to DNA damage may explain different cancer susceptibility between small and large intestine. Exp Biol Med (Maywood). 2005;230:464–471.

32.

Howarth GS, Tooley KL, Davidson GP, Butler RN. A non-invasive method for detection of intestinal mucositis induced by different classes of chemotherapy drugs in the rat. Cancer Biol Ther. 2006;5:1189–1195.PubMed

Titel: Small-Intestinal Manifestations of Dextran Sulfate Sodium Consumption in Rats and Assessment of the Effects of Lactobacillus fermentum BR11
verfasst von: Mark S. Geier
Cassie L. Smith
Ross N. Butler
Gordon S. Howarth
Publikationsdatum: 01.06.2009
Verlag: Springer US
Erschienen in: Digestive Diseases and Sciences / Ausgabe 6/2009
Print ISSN: 0163-2116
Elektronische ISSN: 1573-2568
DOI: https://doi.org/10.1007/s10620-008-0495-4

Leitlinien kompakt für die Innere Medizin

Mit medbee Pocketcards sicher entscheiden.

^{Seit 2022 gehört die medbee GmbH zum Springer Medizin Verlag}

Kostenlos registrieren

Neu im Fachgebiet Innere Medizin

Notfall-TEP der Hüfte ist auch bei 90-Jährigen machbar

26.04.2024 Hüft-TEP Nachrichten

Ob bei einer Notfalloperation nach Schenkelhalsfraktur eine Hemiarthroplastik oder eine totale Endoprothese (TEP) eingebaut wird, sollte nicht allein vom Alter der Patientinnen und Patienten abhängen. Auch über 90-Jährige können von der TEP profitieren.

Niedriger diastolischer Blutdruck erhöht Risiko für schwere kardiovaskuläre Komplikationen

25.04.2024 Hypotonie Nachrichten

Wenn unter einer medikamentösen Hochdrucktherapie der diastolische Blutdruck in den Keller geht, steigt das Risiko für schwere kardiovaskuläre Ereignisse: Darauf deutet eine Sekundäranalyse der SPRINT-Studie hin.

Bei schweren Reaktionen auf Insektenstiche empfiehlt sich eine spezifische Immuntherapie

25.04.2024 Allergien und Intoleranzreaktionen Nachrichten

Insektenstiche sind bei Erwachsenen die häufigsten Auslöser einer Anaphylaxie. Einen wirksamen Schutz vor schweren anaphylaktischen Reaktionen bietet die allergenspezifische Immuntherapie. Jedoch kommt sie noch viel zu selten zum Einsatz.

Therapiestart mit Blutdrucksenkern erhöht Frakturrisiko

25.04.2024 Hypertonie Nachrichten

Beginnen ältere Männer im Pflegeheim eine Antihypertensiva-Therapie, dann ist die Frakturrate in den folgenden 30 Tagen mehr als verdoppelt. Besonders häufig stürzen Demenzkranke und Männer, die erstmals Blutdrucksenker nehmen. Dafür spricht eine Analyse unter US-Veteranen.

Update Innere Medizin

Bestellen Sie unseren Fach-Newsletter und bleiben Sie gut informiert.

Newsletter bestellen

Springer Medizin

Abstract

Bitte loggen Sie sich ein, um Zugang zu diesem Inhalt zu erhalten

Weitere Artikel der Ausgabe 6/2009

Sensitivity of Ultrasonography for Gastric Cancer Diagnosis in Symptomatic Patients

Poor Response to Pegylated Interferon and Ribavirin in Older Women Infected with Hepatitis C Virus of Genotype 1b in High Viral Loads

Influence of Previously Ingested Wheat on Fasting Breath Hydrogen in Celiac Patients

The Effect of Hypericum perforatum (St. John’s Wort) on Experimental Colitis in Rat

Palliation of Malignant Obstructive Jaundice