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Erschienen in:
01.12.2012
Small Liver Remnants Are More Vulnerable to Ischemia/Reperfusion Injury after Extended Hepatectomies: A Case–Control Study
Erschienen in: World Journal of Surgery | Ausgabe 12/2012
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Background
There is evidence that small-for-size liver grafts are more vulnerable to ischemia/reperfusion injury after liver transplantation. We hypothesized that ischemic injury is more pronounced in small liver remnants after major hepatectomies.
Methods
Fifteen patients underwent extended hepatectomy with remnant liver mass less than 30 % of standard liver weight (study group). These patients were matched with patients who underwent minor liver resection, with liver remnants equal to or more than 70 % of standard liver weight (control group). Ischemia/reperfusion injury was assessed by tissue caspase-3 activity postoperatively as well as peak aspartate aminotransferase (AST) values and a-glutathione S-transferase (α-GST) levels adjusted for remnant liver weight. In addition, caspase-3 activity and adjusted serum markers of hepatocyte injury were correlated with the degree of postoperative portal hypertension.
Results
Caspase-3 activity was higher in patients with small liver remnants (22.66 ± 6.57 vs. 12.60 ± 4.06 count per high-power field, p < 0.001). Serum markers of hepatocyte injury, when adjusted per gram of liver remnant, were found to be higher in the study group than in the control group (AST: 1.26 ± 0.25 vs. 0.54 ± 0.11 IU g−1, p < 0.001; α-GST: 0.14 ± 0.02 vs. 0.08 ± 0.01 IU g−1, p < 0.001). Tissue caspase-3 expression in the small liver remnant group correlated with both AST and α-GST levels adjusted per gram of liver remnant (r
2 = 0.51, p = 0.005 and r
2 = 0.71, p < 0.001, respectively). Significant correlations between postoperative portal hypertension and the same markers as well as caspase-3 activity were also demonstrated.
Conclusion
Liver remnants less than 30 % of standard liver weight are much more susceptible to ischemia/reperfusion injury than controls twice the size. Adjustment of serum markers of hepatocyte injury to the liver remnant weight depicts injury more accurately.