Background
Methods
Subjects
Methods
Effect of SEA on cell proliferation
Effect of SEA on HCV replication in PBMC
Reverse transcription – polymerase chain reaction (RT-PCR)
Quantification of intracellular HCV RNA
Results
Detection of HCV in circulating PBMC
Patients | Age | Sex | Genotype | Serum Plus strand | PBMC Plus strand +ve | PBMC Minus strand |
---|---|---|---|---|---|---|
R.F.
|
35
|
♂
|
4
| + | + | + |
M.H.
|
46
|
♂
|
4
| + | + | + |
S.H.
|
45
|
♂
|
4
| + | + | + |
M.AA.
|
40
|
♂
|
4
| + | + | + |
809
|
37
|
♂
|
4
| + | + | - |
K.I.
|
30
|
♂
|
4
| + | + | + |
G.B.
|
37
|
♂
|
4
| + | + | + |
S.A.
|
36
|
♀
|
1b
| + | + | + |
F.AA.
|
42
|
♀
|
4
| + | + | + |
no 47
|
45
|
♂
|
4
| + | + | + |
A.N
|
37
|
♂
|
4
| + | + | + |
M.W.
|
40
|
♂
|
4
| + | + | + |
A.AZ.
|
37
|
♂
|
4
| + | + | - |
Y.AM.
|
42
|
♂
|
1b
| + | + | + |
R.M.
|
42
|
♂
|
4
| + | + | + |
no 51
|
53
|
♂
|
4
| + | + | + |
A.M.
|
47
|
♂
|
4
| + | + | + |
M.A.
|
42
|
♂
|
4
| + | + | + |
AH.AZ.
|
45
|
♂
|
4
| + | + | - |
A 730
|
47
|
♂
|
4
| + | + | + |
A.A.
|
43
|
♂
|
4
| + | + | + |
M.O.
|
45
|
♂
|
4
| + | + | + |
K.M.
|
48
|
♂
|
4
| + | + | + |
A.S.
|
38
|
♂
|
4
| + | + | + |
S.Y.
|
36
|
♂
|
4
| + | + | - |
S.K.
|
43
|
♀
|
4
| + | + | - |
Effect of SEA on PBMC proliferation
Effect of SEA on cultured PBMC count from HCV patients
Effect of SEA on HCV replication in PBMC in vitro
patients | Before stimulation (copies/cell) | After stimulation (copies/cell) | Induction | ||
---|---|---|---|---|---|
Plus | minus | Plus | minus | ||
R.F. | 169 | 196 | 496 | 148 |
I
|
M.H. | 146 | 93 | UD | 119 |
I
|
S.H. | UD | UD | 64 | 64 |
I
|
M.AA. | 125 | UD | UD | UD |
NI
|
809 | 73 | 64 | 79 | 64 |
I
|
K.I. | UD | UD | UD | 122 |
I
|
G.B. | 42 | 51 | 64 | UD |
I
|
S.A. | 185 | UD | 234 | UD |
I
|
F.AA. | 100 | UD | UD | UD |
NI
|
no 47 | 92 | 137 | 126 | UD |
I
|
A.N | UD | UD | UD | 86 |
I
|
M.W. | 100 | 100 | 100 | 105 |
I
|
A.AZ. | UD | 64 | UD | 64 |
NI
|
Y.AM. | 64 | 64 | 64 | 86 |
I
|
R.M. | UD | 100 | 100 | 100 |
NI
|
no 51 | 264 | 264 | 235 | 228 |
NI
|
A.M. | UD | UD | UD | 122 |
I
|
M.A. | 24 | UD | 38 | 84 |
I
|
AH.AZ. | 104 | 100 | 100 | 90 |
NI
|
A 730 | 345 | 404 | 100 | 346 |
NI
|
A.A. | 73 | 73 | 104 | 100 |
I
|
M.O. | 182 | 257 | 185 | 259 |
I
|
K.M. | 73 | 73 | 73 | 79 |
I
|
A.S. | UD | UD | 64 | UD |
I
|
S.Y. | UD | UD | UD | UD |
NI
|
S.K. | UD | UD | UD | UD |
NI
|