nach oben

Clinical and Experimental Medicine

Erschienen in:

19.01.2017 | Original Article

Spatial distribution of mast cells around vessels and glands in human gastric carcinoma

verfasst von: Diego Guidolin, Simona Ruggieri, Tiziana Annese, Cinzia Tortorella, Andrea Marzullo, Domenico Ribatti

Erschienen in: Clinical and Experimental Medicine | Ausgabe 4/2017

Einloggen, um Zugang zu erhalten

Abstract

The spatial distribution of mast cells inside the tumor stroma has been little investigated. In this study, we have evaluated tumor mast cells (MCs) distribution in gastric cancer through the analysis of the morphological features of the spatial patterns generated by these cells, including size, shape, and architecture of the cell pattern. The pattern of distribution of tryptase- and chymase-positive MCs around the blood vessels and gastric glands in human gastric adenocarcinoma samples was investigated by immunohistochemical techniques and by introducing a quantitative approach to characterize the spatial distribution of MCs. In human gastric cancer, both chymase-positive MC and vessels exhibited significant deviations from randomness for what it concerns their spatial relationship with gastric parenchyma. As indicated by cell-to-gland distances shorter than expected by chance, in grade II samples a preferential localization of chymase-positive MC near the gastric glands was observed. Interestingly, the same type of spatial association was exhibited by vessels in grade IV samples, where vessel-to-gland distances shorter than expected by chance were observed. These two findings allow to speculate about a sequence of events in which a subpopulation of MC is first recruited around gastric parenchyma to drive the subsequent development of a vascular support to the tissue.

Ammendola M, Sacco R, Donato G, et al. Mast cell positivity to tryptase correlates with metastatic lymph nodes in gastrointestinal cancer patients treated surgically. Oncology. 2013;85:111–6.CrossRefPubMed

Araya M, Terashima M, Takagane A, et al. Microvessel count predicts metastasis and prognosis in patients with gastric cancer. J Surg Oncol. 1997;65:232–6.CrossRefPubMed

Maeda K, Chung YS, Takatsuka S, et al. Tumour angiogenesis and tumour cell proliferation as prognostic indicators in gastric carcinoma. Br J Cancer. 1995;72:319–23.CrossRefPubMedPubMedCentral

Takahashi Y, Cleary KR, Mai M, Kitadai Y, Bucana CD, Ellis LM. Significance of vessel count and vascular endothelial growth factor and its receptor (KDR) in intestinal-type gastric cancer. Clin Cancer Res. 1996;2:1679–84.PubMed

Tanigawa N, Amaya H, Matsumura M, Shimomatsuya T. Association of tumour vasculature with tumour progression and overall survival of patients with non-early gastric carcinomas. Br J Cancer. 1997;75:566–71.CrossRefPubMedPubMedCentral

Tanigawa N, Amaya H, Matsumura M, Shimomatsuya T. Correlation between expression of vascular endothelial growth factor and tumor vascularity, and patient outcome in human gastric carcinoma. J Clin Oncol. 1997;15:826–32.CrossRefPubMed

Tanigawa N, Amaya H, Matsumura M, et al. Extent of tumor vascularization correlates with prognosis and hematogenous metastasis in gastric carcinomas. Cancer Res. 1996;56:2671–6.PubMed

Maeda K, Y-s Chung, Ogawa Y, et al. Prognostic value of vascular endothelial growth factor expression in gastric carcinoma. Cancer. 1996;77:858–63.CrossRefPubMed

Takahashi Y, Bucana CD, Akagi Y, et al. Significance of platelet-derived endothelial cell growth factor in the angiogenesis of human gastric cancer. Clin Cancer Res. 1998;4:429–34.PubMed

10.

Maehara Y, Kabashima A, Koga T, et al. Vascular invasion and potential for tumor angiogenesis and metastasis in gastric carcinoma. Surgery. 2000;128:408–16.CrossRefPubMed

11.

Zhang H, Wu J, Meng L, Shou CC. Expression of vascular endothelial growth factor and its receptors KDR and Flt-1 in gastric cancer cells. World J Gastroenterol. 2002;8:994–8.CrossRefPubMedPubMedCentral

12.

Ribatti D, Crivellato E. Mast cells and tumours. Berlin: Springer; 2011.CrossRef

13.

Ribatti D, Guidolin D, Marzullo A, et al. Mast cells and angiogenesis in gastric carcinoma. Int J Exp Pathol. 2010;91:350–6.CrossRefPubMedPubMedCentral

14.

Ammendola M, Sacco R, Sammarco G, et al. Mast cells positive to tryptase and c-Kit receptor expressing cells correlates with angiogenesis in gastric cancer patients surgically treated. Gastroenterol Res Pract. 2013;2013:1–5.CrossRef

15.

Guidolin D, Zunarelli E, Genedani S, et al. Opposite patterns of age-associated changes in neurons and glial cells of the thalamus of human brain. Neurobiol Aging. 2008;29:926–36.CrossRefPubMed

16.

Ruifrok AC, Katz RL, Johnston DA. Comparison of quantification of histochemical staining by hue-saturation-intensity (HSI) transformation and color-deconvolution. Appl Immunohisto Mol Morphol. 2003;11:85–91.

17.

Guidolin D, Marinaccio C, Tortorella C, et al. A fractal analysis of the spatial distribution of tumoral mast cells in lymph nodes and bone marrow. Exp Cell Res. 2015;339:96–102.CrossRefPubMed

18.

Russ JC. The image processing handbook, vol. 6. Boca Raton: Taylor & Francis; 2011.

19.

Besag J, Diggle PJ. Simple Monte Carlo tests for spatial pattern. Appl Stat. 1977;26:327.CrossRef

20.

Gatrell AC, Bailey TC, Diggle PJ, Rowlingson BS. spatial point pattern analysis and its application in geographical epidemiology. Trans Inst Br Geogr. 1996;21:256.CrossRef

21.

Philimonenko AA, Janáček J, Hozák P. Statistical evaluation of colocalization patterns in immunogold labeling experiments. J Struct Biol. 2000;132:201–10.CrossRefPubMed

22.

Trabucchi E, Radaelli E, Marazzi M, et al. The role of mast cells in wound healing. Int J Tissue React. 1988;10:367–72.PubMed

23.

Krishna A, Beesley K, Terranova PF. Histamine, mast cells and ovarian function. J Endocrinol. 1989;120:363–71.CrossRefPubMed

24.

Yamada T, Sawatsubashi M, Itoh Y, et al. Localization of vascular endothelial growth factor in synovial membrane mast cells: examination with ”multi-labelling subtraction immunostaining”. Virchows Arch. 1998;433:567–70.CrossRefPubMed

25.

Glowacki J, Mulliken JB. Mast cells in hemangiomas and vascular malformations. Pediatrics. 1982;70:48–51.PubMed

26.

Qu Z, Kayton RJ, Ahmadi P, et al. Ultrastructural immunolocalization of basic fibroblast growth factor in mast cell secretory granules: morphological evidence for bFGF release through degranulation. J Histochem Cytochem. 1998;46:1119–28.CrossRefPubMed

27.

Marinaccio C, Ingravallo G, Gaudio F, et al. Microvascular density, CD68 and tryptase expression in human diffuse large B-cell lymphoma. Leuk Res. 2014;38:1374–7.CrossRefPubMed

28.

Ribatti D, Nico B, Vacca A, et al. Do mast cells help to induce angiogenesis in B-cell non-Hodgkin’s lymphomas? Br J Cancer. 1998;77:1900–6.CrossRefPubMedPubMedCentral

29.

Ribatti D, Vacca A, Marzullo A, et al. Angiogenesis and mast cell density with tryptase activity increase simultaneously with pathological progression in B-cell non-Hodgkin’s lymphomas. Int J Cancer. 2000;85:171–5.CrossRefPubMed

30.

Ribatti D, Vacca A, Nico B, et al. Bone marrow angiogenesis and mast cell density increase simultaneously with progression of human multiple myeloma. Br J Cancer. 1999;79:451–5.CrossRefPubMedPubMedCentral

31.

Ribatti D, Polimeno G, Vacca A, et al. Correlation of bone marrow angiogenesis and mast cells with tryptase activity in myelodysplastic syndromes. Leukemia. 2002;16:1680–4.CrossRefPubMed

32.

Molica S, Vacca A, Crivellato E, Cuneo A, Ribatti D. Tryptase-positive mast cells predict clinical outcome of patients with early B-cell chronic lymphocytic leukemia. Eur J Haematol. 2003;71:137–9.CrossRefPubMed

33.

Bowrey PF, King J, Magarey C, et al. Histamine, mast cells and tumour cell proliferation in breast cancer: does preoperative cimetidine administration have an effect? Br J Cancer. 2000;82:167–70.CrossRefPubMed

34.

Hartveit F. Mast cells and metachromasia in human breast cancer: Their occurrence, significance and consequence: a preliminary report. J Pathol. 1981;134:7–11.CrossRefPubMed

35.

Lachter J, Stein M, Lichtig C, Eidelman S, Munichor M. Mast cells in colorectal neoplasias and premalignant disorders. Dis Colon Rectum. 1995;38:290–3.CrossRefPubMed

36.

Benitez-Bribiesca L, Wong A, Utrera D, Castellanos E. The role of mast cell tryptase in neoangiogenesis of premalignant and malignant lesions of the uterine cervix. J Histochem Cytochem. 2001;49:1061–2.CrossRefPubMed

37.

Guidolin D, Marinaccio C, Tortorella C, et al. Non-random spatial relationships between mast cells and microvessels in human endometrial carcinoma. Clin Exp Med. 2016. doi:10.1007/s10238-016-0407-4.PubMed

38.

Ribatti D, Finato N, Crivellato E, et al. Neovascularization and mast cells with tryptase activity increase simultaneously with pathologic progression in human endometrial cancer. Am J Obstet Gynecol. 2005;193:1961–5.CrossRefPubMed

39.

Dvorak AM, Mihm MC, Osage JE, Dvorak HF. Melanoma. An ultrastructural study of the host inflammatory and vascular responses. J Invest Dermatol. 1980;75:388–93.CrossRefPubMed

40.

Reed JA, McNutt NS, Bogdany JK, Albino AP. Expression of the mast cell growth factor interleukin-3 in melanocytic lesions correlates with an increased number of mast cells in the perilesional stroma: implications for melanoma progression. J Cutan Pathol. 2006;23:495–505.CrossRef

41.

Ribatti D, Vacca A, Ria R, et al. Neovascularisation, expression of fibroblast growth factor-2, and mast cells with tryptase activity increase simultaneously with pathological progression in human malignant melanoma. Eur J Cancer. 2003;39:666–74.CrossRefPubMed

42.

Ullah E, Nagi A, Lail R. Angiogenesis and mast cell density in invasive pulmonary adenocarcinoma. J Cancer Res Ther. 2012;8:537.CrossRefPubMed

43.

Ribatti D, Ennas MG, Vacca A, et al. Tumor vascularity and tryptase-positive mast cells correlate with a poor prognosis in melanoma. Eur J Clin Invest. 2003;33:420–5.CrossRefPubMed

44.

Toth-Jakatics R, Jimi S, Takebayashi S, Kawamoto N. Cutaneous malignant melanoma: correlation between neovascularization and peritumor accumulation of mast cells overexpressing vascular endothelial growth factor. Hum Pathol. 2000;31:955–60.CrossRefPubMed

45.

Diggle PJ, Besag J, Gleaves T (1976) Statistical Analysis of Spatial Point Patterns. Academic Press, London, vol. 1983, pp. 354.

46.

Ripley BD. Tests for randomness for spatial point patterns. J R Stat Soc. 1979;B41:368–74.

47.

Ribatti D, Finato N, Crivellato E, et al. Angiogenesis and mast cells in human breast cancer sentinel lymph nodes with and without micrometastases. Histopathology. 2007;51:837–42.CrossRefPubMed

48.

Starkey JR, Crowle PK, Taubenberger S. Mast-cell-deficient W/Wv mice exhibit a decreased rate of tumor angiogenesis. Int J Cancer. 1988;42:48–52.CrossRefPubMed

49.

Puxeddu I, Piliponsky AM, Bachelet I, Levi-Schaffer F. Mast cells in allergy and beyond. Int J Biochem Cell Biol. 2003;35:1601–7.CrossRefPubMed

50.

Norrby K. Oral administration of a nitric oxide synthase inhibitor enhances de novo mammalian angiogenesis mediated by TNF-alpha, saline and mast-cell secretion. Acta Pathol Microbiol Scand. 2000;108:496–502.CrossRef

51.

Grutzkau A, Kruger-Krasagakes S, Kogel H, Moller A, Lippert U, Henz BM. Detection of intracellular interleukin-8 in human mast cells: flow cytometry as a guide for immunoelectron microscopy. J Histochem Cytochem. 1997;45:935–45.CrossRefPubMed

52.

Qu Z, Liebler JM, Powers MR, et al. Mast cells are a major source of basic fibroblast growth factor in chronic inflammation and cutaneous hemangioma. Am J Pathol. 1995;147:564–73.PubMedPubMedCentral

53.

Grutzkau A, Kruger-Krasagakes S, Baumeister H, et al. Synthesis, storage, and release of vascular endothelial growth factor/vascular permeability factor (VEGF/VPF) by human mast cells: implications for the biological significance of VEGF206. Mol Biol Cell. 1998;9:875–84.CrossRefPubMedPubMedCentral

54.

Muramatsu M, Katada J, Hattori M, Hayashi I, Majima M. Chymase mediates mast cell-induced angiogenesis in hamster sponge granulomas. Eur J Pharmacol. 2000;402:181–91.CrossRefPubMed

55.

Ribatti D, Ranieri G, Nico B, Benagiano V, Crivellato E. Tryptase and chymase are angiogenic in vivo in the chorioallantoic membrane assay. Int J Dev Biol. 2011;55:99–102.CrossRefPubMed

Titel: Spatial distribution of mast cells around vessels and glands in human gastric carcinoma
verfasst von: Diego Guidolin
Simona Ruggieri
Tiziana Annese
Cinzia Tortorella
Andrea Marzullo
Domenico Ribatti
Publikationsdatum: 19.01.2017
Verlag: Springer International Publishing
Erschienen in: Clinical and Experimental Medicine / Ausgabe 4/2017
Print ISSN: 1591-8890
Elektronische ISSN: 1591-9528
DOI: https://doi.org/10.1007/s10238-017-0452-7

Leitlinien kompakt für die Innere Medizin

Mit medbee Pocketcards sicher entscheiden.

^{Seit 2022 gehört die medbee GmbH zum Springer Medizin Verlag}

Kostenlos registrieren

Neu im Fachgebiet Innere Medizin

Notfall-TEP der Hüfte ist auch bei 90-Jährigen machbar

26.04.2024 Hüft-TEP Nachrichten

Ob bei einer Notfalloperation nach Schenkelhalsfraktur eine Hemiarthroplastik oder eine totale Endoprothese (TEP) eingebaut wird, sollte nicht allein vom Alter der Patientinnen und Patienten abhängen. Auch über 90-Jährige können von der TEP profitieren.

Niedriger diastolischer Blutdruck erhöht Risiko für schwere kardiovaskuläre Komplikationen

25.04.2024 Hypotonie Nachrichten

Wenn unter einer medikamentösen Hochdrucktherapie der diastolische Blutdruck in den Keller geht, steigt das Risiko für schwere kardiovaskuläre Ereignisse: Darauf deutet eine Sekundäranalyse der SPRINT-Studie hin.

Bei schweren Reaktionen auf Insektenstiche empfiehlt sich eine spezifische Immuntherapie

25.04.2024 Allergien und Intoleranzreaktionen Nachrichten

Insektenstiche sind bei Erwachsenen die häufigsten Auslöser einer Anaphylaxie. Einen wirksamen Schutz vor schweren anaphylaktischen Reaktionen bietet die allergenspezifische Immuntherapie. Jedoch kommt sie noch viel zu selten zum Einsatz.

Therapiestart mit Blutdrucksenkern erhöht Frakturrisiko

25.04.2024 Hypertonie Nachrichten

Beginnen ältere Männer im Pflegeheim eine Antihypertensiva-Therapie, dann ist die Frakturrate in den folgenden 30 Tagen mehr als verdoppelt. Besonders häufig stürzen Demenzkranke und Männer, die erstmals Blutdrucksenker nehmen. Dafür spricht eine Analyse unter US-Veteranen.

Update Innere Medizin

Bestellen Sie unseren Fach-Newsletter und bleiben Sie gut informiert.

Newsletter bestellen

Springer Medizin

Abstract

Bitte loggen Sie sich ein, um Zugang zu diesem Inhalt zu erhalten

Weitere Artikel der Ausgabe 4/2017

New single nucleotide polymorphisms (SNPs) in homologous recombination repair genes detected by microarray analysis in Polish breast cancer patients

Upregulation of ROCK2 in gastric cancer cell promotes tumor cell proliferation, metastasis and invasion

Expression of Bruton’s tyrosine kinase in B-cell neoplasms evaluated by flow cytometry

Relationship between IL-27 and coronary arterial lesions in children with Kawasaki disease

The use of frozen plasma samples in thromboelastometry