Background
Methods
Bacterial strains and drug susceptibility testing
Determination of MIC
RNA extraction and reverse transcription
Quantification of gene expression using real-time quantitative PCR
Statistical analysis
Results
M. tuberculosis strains | INH | RIF | STR | EMB | KAN | OFX | MIC of RIF mg/L |
---|---|---|---|---|---|---|---|
1 | S | R | S | S | S | S | 256 |
2 | S | R | S | S | S | S | 256 |
3 | R | R | R | R | S | S | 256 |
4 | R | R | R | S | S | S | 256 |
5 | R | R | R | S | S | S | 256 |
6 | R | R | S | S | S | R | 256 |
7 | R | R | R | R | S | R | 256 |
8 | R | R | S | R | S | S | 256 |
9 | R | R | R | R | R | S | 256 |
10 | R | R | R | R | R | S | 256 |
11 | R | R | R | R | S | R | 256 |
12 | R | R | R | R | R | R | 256 |
13 | R | R | R | R | R | S | 256 |
14 | R | R | R | R | R | S | 256 |
15 | R | R | R | R | S | R | 256 |
M. tuberculosis strains |
rpfA
|
rpfB
|
rpfC
|
rpfD
|
rpfE
|
---|---|---|---|---|---|
1 |
1.073
*
| 0.765 |
1.191
*
|
20.580
**
| 0.973 |
2 | 0.451 | 0.746 |
3.976
**
|
1.600
*
| 0.838 |
3 |
1.249
*
| 0.650 | 0.637 |
1.211
*
|
1.009
*
|
4 |
1.165
*
| 0.633 |
1.174
*
|
1.724
*
|
2.575
**
|
5 | 0.493 |
2.644
**
|
5.275
**
|
2.854
**
|
1.356
*
|
6 | 0.767 | 0.760 | 0.901 |
1.642
*
|
1.022
*
|
7 | 0.567 | 0.956 |
1.361
*
| 0.790 | 0.970 |
8 | 0.781 |
1.298
*
| 0.498 |
26.750
*
|
2.017
**
|
9 |
0.410
*
|
13.390
**
|
6.726
**
|
1.399
*
|
1.904
*
|
10 |
1.309
*
|
1.170
*
|
1.324
*
| 0.641 | 0.771 |
11 | – |
0.398
*
|
1.168
*
| 0.752 | 0.932 |
12 | 0.727 |
1.087
*
| 0.671 |
1.206
*
|
1.021
*
|
13 |
1.458
*
| 0.986 | 0.655 | 0.978 |
1.250
*
|
14 |
1.432
*
|
0.385
*
|
1.542
*
|
1.344
*
|
1.198
*
|
15 |
1.738
*
| 0.789 | 0.475 | 0.834 | 0.665 |