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Journal of Assisted Reproduction and Genetics

Erschienen in:

01.05.2014 | Gamete Biology

Syncytin-1 and its receptor is present in human gametes

verfasst von: B. Bjerregaard, J. G. Lemmen, M. R. Petersen, E. Østrup, L. H. Iversen, K. Almstrup, L.-I. Larsson, S. Ziebe

Erschienen in: Journal of Assisted Reproduction and Genetics | Ausgabe 5/2014

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Abstract

Main purpose and research question

To determine whether the true fusogen Syncytin-1 and its receptor (ASCT-2) is present in human gametes using qRT-PCR, immunoblotting and immunofluorescence.

Methods

Donated oocytes and spermatozoa, originating from a fertility center in tertiary referral university hospital, underwent qRT-PCR, immunoblotting and immunofluorescence analyzes.

Results

Quantitative RT-PCR of sperm samples from sperm donors showed that syncytin-1 is present in all samples, however, protein levels varied between donors. Syncytin-1 immunoreactivity predominates in the sperm head and around the equatorial segment. The receptor ASCT-2 is expressed in the acrosomal region and in the sperm tail. Moreover, ASCT-2, but not syncytin-1, is expressed in oocytes and the mRNA level increases with increasing maturity of the oocytes.

Conclusions

Syncytin and its receptor are present in human gametes and localization and temporal appearance is consistent with a possible role in fusion between oocyte and sperm.

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Titel: Syncytin-1 and its receptor is present in human gametes
verfasst von: B. Bjerregaard
J. G. Lemmen
M. R. Petersen
E. Østrup
L. H. Iversen
K. Almstrup
L.-I. Larsson
S. Ziebe
Publikationsdatum: 01.05.2014
Verlag: Springer US
Erschienen in: Journal of Assisted Reproduction and Genetics / Ausgabe 5/2014
Print ISSN: 1058-0468
Elektronische ISSN: 1573-7330
DOI: https://doi.org/10.1007/s10815-014-0224-1

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Syncytin-1 and its receptor is present in human gametes