Introduction
Materials and Methods
Patients and Samples
TREC Test
Extraction of DNA
TREC qRT-PCR
TREC forward | 5′-CAC ATC CCT TTC AAC CAT GCT-3′ |
TREC reverse | 5’GCC AGC TGC AGG GTT TAG G-3′ |
TREC probe | 5′-FAM-ACA CCT CTG GTT TTT GTA AAG GTG CCC ACT-3′ TAMRA |
β-actin forward | 5′-ATT TCC CTC TCA GGC ATG GA-3′ |
β-actin reverse | |
5′-CGT CAC ACT TCA TGA TGG AGT TG-3′ | |
β-actin probe | 5′- FAM-GTG GCA TCC ACG AAA CTA-3′-TAMRA |
Statistical Analysis
Results
TREC Values in Different Groups of Heart Defect
Non-conotruncal | TREC/µLa | Conotruncal | TREC/µL | 22q11.2 del with conotruncal | TREC/µL | |
---|---|---|---|---|---|---|
Heart defects | Atrioventricular septal defect (nb = 1) | 252 | Truncus arteriosus (n = 3) | 261 (134–363) | Truncus arteriosus (n = 1) | 74 |
Atrial septal defect (n = 25) | 330 (110–595) | Transposition of the great arteries (n = 19) | 289 (75–795) | Simple septal defects (n = 4) | 143 (84–241) | |
Valvular pulmonary stenosis (n = 14) | 308 (124–635) | Tetralogy of fallot (n = 5) | 285 (238–380) | Tetralogy of fallot (n = 8) | 71 (23–215) | |
Aortic stenosis (n = 17) | 331(75–627) | Pulmonary atresia with ventricular septal defect (n = 5) | 230 (138–326) | Pulmonary atresia with ventricular septal defect (n = 4) | 74 (84–161) | |
Pulmonary atresia without ventricular septal defect (n = 3) | 250 (189–317) | Pulmonary atresia without ventricular septal defect (n = 1) | 53 | |||
Interrupted aortic arch (n = 6) | 275 (7–548) | Interrupted aortic arch (n = 6) | 113 (0–190) | |||
Congenital corrected transposition of the great arteries (n = 1) | 102 | Absent pulmonary valve, overriding aorta, ventricular septal defect (n = 1) | 84 |
Non-conotruncal | Conotruncal | 22q11.2 del with conotruncal | |
---|---|---|---|
Mean TREC | 323 TREC/µLa | 270 TREC/µL | 94 TREC/µL |
Operated within 14 days | 10b | 35 | 12 |
Died | 0 | 3 | 4 |
Hospitalization due to infections | 13/47c | 17/31 | 14/25 |
TREC Values and Heart Surgery/Intervention/Fatal Outcome
TREC Values and Prematurity, Birth Weight, and Delivery Mode
Heart defects | Premature infants | Gestational age | TREC values/ µL | Diagnosis |
---|---|---|---|---|
Non-conotruncal | 1 | 34 | 120 | ASD |
2 | 34 | 110 | ASD | |
3 | 36 | 220 | VPS | |
4 | 35 | 234 | ASD | |
5 | 34 | 302 | ASD | |
6 | 31 | 595 | ASD | |
Conotruncal | 7 | 36 | 102 | CCTGA |
Conotruncal with 22q11.2 del | 8 | 34 | 161 | PA, VSD |
9 | 36 | 141 | IAA | |
10 | 35 | 36 | IAA | |
11 | 34 | 53 | PA, VSD | |
12 | 33 | 40 | TOF |