T cell unresponsiveness in a pediatric cystic fibrosis patient: a case report

Born at term in southern Ontario, Canada, of non-consanguineous Caucasian parents following an uneventful pregnancy, the patient was appropriate in size for a full term baby and was discharged from hospital at four hours of age. She was initially breast fed but readmitted to hospital on the third day of life for failure to pass meconium. Intestinal perforation was noted and about 15 cm of bowel were resected. A diagnosis of CF was confirmed by repeated sweat chloride tests, consistent with her CFTR genotype of F508del homozygote. She has been followed regularly at the multidisciplinary CF Clinic of The Hospital for Sick Children, Toronto, on a quarterly basis at a minimum. Maintaining adequate weight gain has been a challenge. Although respiratory symptoms were not recognized in the immediate newborn period, at three months she had a severe pulmonary infection with Respiratory Syncitial Virus. By the age of 9 months, her chest X-Ray had early bronchiectatic changes that progressed to diffuse cystic bronchiectasis at 12 years. Her respiratory secretions intermittently grew Pseudomonas aeruginosa since the age of two years, Staphylococcus aureus since four years, as well as (less frequently) Haemophilus influenzae and Stenotrophomonas maltophilia. Although Aspergillus fumigatus has been identified several times, her serum IgE levels and skin tests for aspergillus allergy have been consistently normal, arguing against a diagnosis of allergic bronchopulmonary aspergillosis. From an early age, her pulmonary function has been more compromised than that of her birth cohort peers at our institution. Despite aggressive treatment of an increasingly frequent series of acute pulmonary exacerbations, her FEV₁ during her 18th year was consistently below 30 and for several months she required 3 - 4 L/min of supplemental oxygen to maintain her hemoglobin oxygen saturations over 95%. Until her lung transplantation just before her 18th birthday, she had not been on systemic corticosteroids or other immunosuppressive agents. Her serology had been negative for IgG antibody to cytomegalovirus (CMV) by enzyme immunoassay (5 times out of 5 assessments over the last 15 months) and also negative when assayed (on at least one occasion) for IgG antibodies against each of Epstein-Barr virus early or nuclear antigens, hepatitis C virus, human immunodeficiency viruses 1 or 2, herpes simplex virus, or Toxoplasma gondii. Her most recent serology was also negative for IgG antibody to Epstein-Barr virus capsid antigen, although she had been positive for this antibody once over a year ago. No evidence was found for adenoviral DNA by polymerase chain reaction, or for hepatitis B surface antigen or HIV P24 antigen by chemiluminescence immunoassay. No antigens of the following respiratory viruses were detected on immunofluorescence microscopy of respiratory secretions on nasopharyngeal swabs: parainfluenza viruses 1, 2, 3, adenovirus, respiratory syncytial virus, human metapneumovirus, influenza viruses A, B. Nevertheless, we have evidence of good antibody responses to her vaccines against measles, mumps, rubella, varicella zoster virus and hepatitis B.

We carried out immunological analysis of T cell response in the peripheral blood of this patient prior to her lung transplantation. Peripheral blood mononuclear cells were isolated and stimulated with anti-CD3 and anti-CD28 antibodies to assess for cytokine production from T cells [10]. Whereas T cells amongst the mononuclear cells from healthy controls produced identifiable levels of IFN-γ which could be detected by flow cytometry [11], T cells from the patient failed to show any IFN-γ production (see Figure 1A). This could either be explained by massive immunosuppression within the immune system of the patient or an unlikely scenario in which her T cells are intrinsically defective in cytokine production. In order to test for this scenario, we isolated and carried out differentiation of naïve CD4+ T cells into inflammatory T helper type 1 (Th1) or T helper type 17 (Th17) lineages, or into regulatory T cells (Tregs), using exogenous cytokines [12, 13]. Although T cells from healthy controls were able to differentiate into Th1 and Th17 lineages, detected by production of IFN-γ and Th17 respectively, along with differentiation into Tregs detected by expression of the transcription actor Foxp3 [14], T cells from the patient showed a complete block in T cell differentiation into the three T cell lineages tested [12, 13], pointing towards an intrinsic loss of T cell responsiveness to differentiate in the presence of exogenous cytokines (Figures 1B, C). Furthermore, this block in T cell differentiation was not specific for one particular lineage but was spread across all three lineages. Loss of responsiveness to exogenous cytokines led us to hypothesize that the patient could be demonstrating T cell exhaustion.

T cell exhaustion is a condition of progressive loss of T cell function, which was initially reported as clonal deletion of virus-specific T cells during high grade chronic CMV infections [15, 16]. Gradual improvements in methodology for assessing T cell exhaustion identified that there was no clonal deletion of T cells but rather the T cells became unable to respond to stimuli due to sustained expression of inhibitory receptors that prevent effector function of T cells [15, 16]. In addition to CMV infections, T cell exhaustion has also been reported following chronic exposure to HIV and/or Hepatitis C [17], Hepatitis B [18], Plasmodium falciparum[19] and Toxoplasma gondii (reviewed in [20]), but we have no evidence to suggest that our patient has been chronically infected by any of these pathogens. To the best of our knowledge, T cell exhaustion has not been documented in CF.

We hypothesized that the patient demonstrated T cell exhaustion, whereby even naïve T cells were inept at undergoing differentiation. In order to test this hypothesis, we assessed the levels of T cell exhaustion markers, LAG-3 [21] and CTLA-4 [16], on unstimulated as well as stimulated CD4+ T cells. Although LAG-3 and CTLA-4 are not expressed on naïve T cells from healthy individuals [22, 23], freshly isolated naïve CD4+ T cells from peripheral blood of our present patient showed sustained expression of LAG-3 and CTLA-4, which were further induced even upon ex vivo stimulation with cytokines that drive T cell proliferation (Figure 2). These findings point towards T cell exhaustion as being the cause of the failure of the patient’s T cells to undergo differentiation in response to cytokine stimulation.

Circulating T lymphocytes are known to have defects in CFTR expression in CF [24]. Generally, reported abnormalities of T cells in CF have involved increased immune responses, especially relating to Th17 helper T cells, which have been linked to CF pulmonary exacerbations [9, 25] and to neutrophilia very early in life [26]. Elevated sputum levels of Th17 cytokines are associated with active CF lung infections with P. aeruginosa. Elevated IL-17 levels predict future acquisition of P. aeruginosa infections [8]. Again, to the best of our knowledge T cell exhaustion has not been documented in CF.

We proceeded to also study another teen-aged girl with CF in our clinic, patient B, who had a very similar degree of lung dysfunction (e.g., FEV₁ chronically < 30% of the predicted value) and who also underwent double lung transplantation since the initial submission of this report. Unlike the subject of our present report (patient A), whose T cells demonstrated a marked induction of the T cell exhaustion markers LAG-3 and CTLA-4, pre-transplant patient B showed only a mild induction of LAG-3 and CTLA-4 (Figure 2). Patient B also more closely resembled control than patient A, in that patient B showed Th1 and Th17 differentiation (Figures 1B, C), but compared to control showed somewhat more Th17 and less Th1, consistent with our recent report of an innate predisposition of naïve CF helper T cells to differentiate towards the Th17 phenotype [27]. Thus we cannot suggest that the T cell exhaustion described in patient A is an event that occurs in all patients at this stage of CF lung disease. It remains unknown if T cell exhaustion in CF is unique to patient A.