Erschienen in:
01.12.2001 | Paper Report
Targeted arthritis gene therapy with T cells
verfasst von:
David Gould
Erschienen in:
Arthritis Research & Therapy
|
Ausgabe 1/2000
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Excerpt
CD4
+ T cells have tissue-specific homing properties and are observed in target organs in autoimmunity. As such, these cells could be utilised as carrier cells to achieve local expression of immunoregulatory genes at disease sites.
IL-12 is a heterodimeric cytokine that promotes Th1 differentiation of T cells. The p40 subunit of IL-12 expressed by itself, however, is an antagonist of IL-12 function and therefore has potential therapeutic benefit in arthritis. To provide direct evidence of T cell homing to disease sites in the mouse collagen-induced arthritis (CIA) model, the authors have utilised collagen type II (CII)-specific primary T cells and hybridomas derived from CII-specific
TCR transgenic mice. Cells engineered to express reporter genes enabled analysis of
in vivo localisation, and cells transduced to express IL-12 p40 were assessed for therapeutic effect. …
