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01.11.2008 | ORIGINAL CONTRIBUTION

TGF-β1 enhances cardiomyogenic differentiation of skeletal muscle-derived adult primitive cells

verfasst von: Ahmed Abdel-Latif, MD, Ewa K. Zuba-Surma, PhD, Jamie Case, PhD, Sumit Tiwari, MD, Greg Hunt, BS, Smita Ranjan, BS, Robert J. Vincent, BS, Edward F. Srour, PhD, Roberto Bolli, MD, Buddhadeb Dawn, MD

Erschienen in: Basic Research in Cardiology | Ausgabe 6/2008

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Abstract

The optimal medium for cardiac differentiation of adult primitive cells remains to be established. We quantitatively compared the efficacy of IGF-1, dynorphin B, insulin, oxytocin, bFGF, and TGF-β1 in inducing cardiomyogenic differentiation. Adult mouse skeletal muscle-derived Sca1+/CD45-/c-kit-/Thy-1+ (SM+) and Sca1-/CD45-/c-kit-/Thy-1+ (SM-) cells were cultured in basic medium (BM; DMEM, FBS, IGF-1, dynorphin B) alone and BM supplemented with insulin, oxytocin, bFGF, or TGF-β1. Cardiac differentiation was evaluated by the expression of cardiac-specific markers at the mRNA (qRT-PCR) and protein (immunocytochemistry) levels. BM+TGF-β1 upregulated mRNA expression of Nkx2.5 and GATA-4 after 4 days and Myl2 after 9 days. After 30 days, BM+TGF-β1 induced the greatest extent of cardiac differentiation (by morphology and expression of cardiac markers) in SM- cells. We conclude that TGF-β1 enhances cardiomyogenic differentiation in skeletal muscle-derived adult primitive cells. This strategy may be utilized to induce cardiac differentiation as well as to examine the cardiomyogenic potential of adult tissue-derived stem/progenitor cells.

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Titel: TGF-β1 enhances cardiomyogenic differentiation of skeletal muscle-derived adult primitive cells
verfasst von: Ahmed Abdel-Latif, MD
Ewa K. Zuba-Surma, PhD
Jamie Case, PhD
Sumit Tiwari, MD
Greg Hunt, BS
Smita Ranjan, BS
Robert J. Vincent, BS
Edward F. Srour, PhD
Roberto Bolli, MD
Buddhadeb Dawn, MD
Publikationsdatum: 01.11.2008
Verlag: D. Steinkopff-Verlag
Erschienen in: Basic Research in Cardiology / Ausgabe 6/2008
Print ISSN: 0300-8428
Elektronische ISSN: 1435-1803
DOI: https://doi.org/10.1007/s00395-008-0729-9

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