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Inflammation
Erschienen in: Inflammation 2/2010

01.04.2010

The Effect of Epigallocatechin Gallate on Lipopolysaccharide-Induced Acute Lung Injury in a Murine Model

verfasst von: Hong-Beom Bae, Mei Li, Jong-Phil Kim, Seok-Jai Kim, Cheol-Won Jeong, Hyung-Gon Lee, Woong-Mo Kim, Hyung-Seok Kim, Sang-Hyun Kwak

Erschienen in: Inflammation | Ausgabe 2/2010

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Abstract

This study was performed to evaluate the effects of epigallocatechin 3 gallate (EGCG) on lipopolysaccharide (LPS)-induced acute lung injury in a murine model. In the present study, production of TNF-α and MIP-2 and activation of extracellular signal-regulated kinases (ERK)1/2, c-Jun amino terminal kinases (JNK) and p38 in RAW264.7 cells were measured. EGCG inhibited the production of TNF-α and MIP-2, and attenuated phosphorylation levels of ERK1/2 and JNK, but not p38 in RAW264.7 cells stimulated with LPS. Also, EGCG attenuated the production of TNF-α and MIP-2, and the phosphorylation of ERK1/2 and JNK in the lungs of mice administered with LPS intratracheally. It reduced wet/dry weight ratio, histological severities, and neutrophil accumulation in the lungs in mice given LPS. Our results showed that EGCG attenuated LPS-induced lung injury by suppression of the MIP-2 and TNF-α production, and ERK1/2 and JNK activation in macrophage stimulated with LPS.
Literatur
1.
Ware, L.B., and M.A. Matthay. 2000. The acute respiratory distress syndrome. New England Journal of Medicine 342: 1334–1349.CrossRefPubMed
2.
Kollef, M.H., and D.P. Schuster. 1995. The acute respiratory distress syndrome. New England Journal of Medicine 332: 27–37.CrossRefPubMed
3.
Knaus, W.A., X. Sun, R.B. Hakim, and D.P. Wagner. 1994. Evaluation of definitions for adult respiratory distress syndrome. American Journal of Respiratory and Critical Care Medicine 150: 311–317.PubMed
4.
Repine, J.E. 1992. Scientific perspective on adult respiratory distress syndrome. Lancet 339: 466–469.CrossRefPubMed
5.
Puneet, P., S. Moochhala, and M. Bhatia. 2005. Chemokines in acute respiratory distress syndrome. American Journal of Physiology. Lung Cellular and Molecular Physiology 288: L3–L15.CrossRefPubMed
6.
Martich, G.D., R.L. Danner, M. Ceska, and A.F. Suffredini. 1991. Detection of interleukin 8 and tumor necrosis factor in normal humans after intravenous endotoxin: The effect of anti-inflammatory agents. Journal of Experimental Medicine 173: 1021–1024.CrossRefPubMed
7.
Goodman, R.B., R.M. Strieter, C.W. Frevert, C.J. Cummings, P. Tekamp-Olson, S.L. Kunkel, A. Walz, and T.R. Martin. 1998. Quantitative comparison of C–X–C chemokines produced by endotoxin-stimulated human alveolar macrophages. American Journal of Physiology. Lung Cellular and Molecular Physiology 275: L87–L95.
8.
Schnaitman, C.A., and J.D. Klena. 1993. Genetics of lipopolysaccharide biosynthesis in enteric bacteria. Microbiological Reviews 57: 655–682.PubMed
9.
Suffredini, A.F., R.E. Fromm, M.M. Parker, M. Brenner, J.A. Kovacs, R.A. Wesley, and J.E. Parrillo. 1989. The cardiovascular response of normal humans to the administration of endotoxin. New England Journal of Medicine 321: 280–287.PubMedCrossRef
10.
Szarka, R.J., N. Wang, L. Gordon, P.N. Nation, and R.H. Smith. 1997. A murine model of pulmonary damage induced by lipopolysaccharide via intranasal instillation. Journal of Immunological Methods 202: 49–57.CrossRefPubMed
11.
Menezes, S.L., P.T. Bozza, H.C. Neto, A.P. Laranjeira, E.M. Negri, V.L. Capelozzi, W.A. Zin, and P.R. Rocco. 2005. Pulmonary and extrapulmonary acute lung injury: Inflammatory and ultrastructural analyses. Journal of Applied Physiology 98: 1777–1783.CrossRefPubMed
12.
Itoh, T., H. Obata, S. Murakami, K. Hamada, K. Kangawa, H. Kimura, and N. Nagaya. 2007. Adrenomedullin ameliorates lipopolysaccharide-induced acute lung injury in rats. American Journal of Physiology. Lung Cellular and Molecular Physiology 293: L446–L452.CrossRefPubMed
13.
Speyer, C.L., T.A. Neff, R.L. Warner, R.F. Guo, J.V. Sarma, N.C. Riedemann, M.E. Murphy, H.S. Murphy, and P.A. Ward. 2003. Regulatory effects of iNOS on acute lung inflammatory responses in mice. American Journal of Pathology 163: 2319–2328.PubMed
14.
Shi, L., R. Kishore, M.R. Mcmullen, and L.E. Nagy. 2002. Lipopolysaccharide stimulation of ERK1/2 increases TNF-alpha production via Egr-1. American Journal of Physiology. Cell Physiology 282: C1205–C1211.PubMed
15.
Kwak, H.J., J.S. Song, J.Y. Heo, S.D. Yang, J.Y. Nam, and H.G. Cheon. 2005. Roflumilast inhibits lipopolysaccharide-induced inflammatory mediators via suppression of nuclear factor-kappa B, p38 mitogen-activated protein kinase, and c-Jun NH2-terminal kinase activation. Journal of Pharmacology and Experimental Therapeutics 315: 1188–1195.CrossRefPubMed
16.
Kim, H.Y., and H.S. Kim. 2007. Upregulation of MIP-2 (CXCL2) expression by 15-deoxy-Delta(12,14)-prostaglandin J(2) in mouse peritoneal macrophages. Immunology and Cell Biology 85: 60–67.CrossRefPubMed
17.
Xu, Z., C.X. Huang, Y. Li, P.Z. Wang, G.L. Ren, C.S. Chen, F.J. Shang, Y. Zhang, Q.Q. Liu, Z.S. Jia, Q.H. Nie, Y.T. Sun, and X.F. Bai. 2007. Toll-like receptor 4 siRNA attenuates LPS-induced secretion of inflammatory cytokines and chemokines by macrophages. Journal of Infection 55: e1–e9.CrossRefPubMed
18.
Lin, J.K., Y.C. Liang, and S.Y. Lin-Shiau. 1999. Cancer chemoprevention by tea polyphenols through mitotic signal transduction blockade. Biochemical pharmacology 58: 911–915.CrossRefPubMed
19.
Serafini, M., A. Ghiselli, and A. Ferro-Luzzi. 1996. In vivo antioxidant effect of green and black tea in man. European Journal of Clinical Nutrition 50: 28–32.PubMed
20.
Donà, M., I. Dell’aica, F. Calabrese, R. Benelli, M. Morini, A. Albini, and S. Garbisa. 2003. Neutrophil restraint by green tea: Inhibition of inflammation, associated angiogenesis, and pulmonary fibrosis. Journal of Immunology 170: 4335–4341.
21.
Yang, F., W.J. De Villiers, C.J. Mcclain, and G.W. Varilek. 1998. Green tea polyphenols block endotoxin-induced tumor necrosis factor-production and lethality in a murine model. Journal of Nutrition 128: 2334–2340.PubMed
22.
Okabe, S., Y. Ochiai, M. Aida, K. Park, S.J. Kim, T. Nomura, M. Suganuma, and H. Fujiki. 1999. Mechanistic aspects of green tea as a cancer preventive: Effect of components on human stomach cancer cell lines. Japanese Journal of Cancer Research 90: 733–739.PubMed
23.
Rogers, J., I. Perkins, A. Van Olphen, N. Burdash, T.W. Klein, and H. Friedman. 2005. Epigallocatechin gallate modulates cytokine production by bone marrow-derived dendritic cells stimulated with lipopolysaccharide or muramyldipeptide, or infected with Legionella pneumophila. Experimental Biology and Medicine (Maywood) 230: 645–651.
24.
Shin, H.Y., S.H. Kim, H.J. Jeong, S.Y. Kim, T.Y. Shin, J.Y. Um, S.H. Hong, and H.M. Kim. 2007. Epigallocatechin-3-gallate inhibits secretion of TNF-alpha, IL-6 and IL-8 through the attenuation of ERK and NF-kappa B in HMC-1 cells. International Archives of Allergy and Immunology 142: 335–344.CrossRefPubMed
25.
Kim, I.B., D.Y. Kim, S.J. Lee, M.J. Sun, M.S. Lee, H. Li, J.J. Cho, and C.S. Park. 2006. Inhibition of IL-8 production by green tea polyphenols in human nasal fibroblasts and a549 epithelial cells. Biological & Pharmaceutical Bulletin 29: 1120–1125.CrossRef
26.
Porath, D., C. Riegger, J. Drewe, and J. Schwager. 2005. Epigallocatechin-3-gallate impairs chemokine production in human colon epithelial cell lines. Journal of Pharmacology and Experimental Therapeutics 315: 1172–1180.CrossRefPubMed
27.
Netsch, M.I., H. Gutmann, C. Aydogan, and J. Drewe. 2006. Green tea extract induces interleukin-8 (IL-8) mRNA and protein expression but specifically inhibits IL-8 secretion in caco-2 cells. Planta Medica 72: 697–702.CrossRefPubMed
28.
Yun, H.J., W.H. Yoo, M.K. Han, Y.R. Lee, J.S. Kim, and S.I. Lee. 2008. Epigallocatechin-3-gallate suppresses TNF-alpha-induced production of MMP-1 and -3 in rheumatoid arthritis synovial fibroblasts. Rheumatology International 29: 23–29.CrossRefPubMed
29.
Ichikawa, D., A. Matsui, M. Imai, Y. Sonoda, and T. Kasahara. 2004. Effect of various catechins on the IL-12p40 production by murine peritoneal macrophages and a macrophage cell line, J774.1. Biological & Pharmaceutical Bulletin 27: 1353–1358.CrossRef
30.
Kitamura, Y., S. Hashimoto, N. Mizuta, A. Kobayashi, K. Kooguchi, I. Fujiwara, and H. Nakajima. 2001. Fas/FasL-dependent apoptosis of alveolar cells after lipopolysaccharide-induced lung injury in mice. American Journal of Respiratory and Critical Care Medicine 163: 762–769.PubMed
31.
Ito, Y., T. Betsuyaku, Y. Nasuhara, and M. Nishimura. 2007. Lipopolysaccharide-induced neutrophilic inflammation in the lungs differs with age. Experimental Lung Research 33: 375–384.CrossRefPubMed
32.
Zhang, Y.L., and C. Dong. 2005. MAP kinases in immune responses. Cellular and Molecular Immunology 2: 20–27.PubMed
33.
Darieva, Z., E.B. Lasunskaia, M.N. Campos, T.L. Kipnis, and W.D. Da Silva. 2004. Activation of phosphatidylinositol 3-kinase and c-Jun-N-terminal kinase cascades enhances NF-kappa B-dependent gene transcription in BCG-stimulated macrophages through promotion of p65/p300 binding. Journal of Leukocyte Biology 75: 689–697.CrossRefPubMed
34.
Geppert, T.D., C.E. Whitehurst, P. Thompson, and B. Beutler. 1994. Lipopolysaccharide signals activation of tumor necrosis factor biosynthesis through the ras/raf-1/MEK/MAPK pathway. Molecular Medicine 1: 93–103.PubMed
35.
Chen, G., J. Li, M. Ochani, B. Rendon-Mitchell, X. Qiang, S. Susarla, L. Ulloa, H. Yang, S. Fan, S.M. Goyert, P. Wang, K.J. Tracey, A.E. Sama, and H. Wang. 2004. Bacterial endotoxin stimulates macrophages to release HMGB1 partly through CD14- and TNF-dependent mechanisms. Journal of Leukocyte Biology 76: 994–1001.CrossRefPubMed
36.
Means, T.K., R.P. Pavlovich, D. Roca, M.W. Vermeulen, and M.J. Fenton. 2000. Activation of TNF-alpha transcription utilizes distinct MAP kinase pathways in different macrophage populations. Journal of Leukocyte Biology 67: 885–893.PubMed
37.
Weber, S.M., J.M. Chen, and S.M. Levitz. 2002. Inhibition of mitogen-activated protein kinase signaling by chloroquine. Journal of Immunology 168: 5303–5309.
38.
Wang, S.I., and H. Mukhtar. 2002. Gene expression profile in human prostate LNCaP cancer cells by (−)epigallocatechin-3-gallate. Cancer Letter 182: 43–51.CrossRef
39.
Lu, J., A. Gosslau, A.Y. Liu, and K.Y. Chen. 2008. PCR differential display-based identification of regulator of G protein signaling 10 as the target gene in human colon cancer cells induced by black tea polyphenol theaflavin monogallate. European Journal of Pharmacology 601: 66–72.CrossRefPubMed
40.
Coso, O.A., H. Teramoto, W.F. Simonds, and J.S. Gutkind. 1996. Signaling from G protein-coupled receptors to c-Jun kinase involves beta gamma subunits of heterotrimeric G proteins acting on a Ras and Rac1-dependent pathway. Journal of Biological Chemistry 271: 3963–3966.CrossRefPubMed
41.
Crespo, P., N. Xu, W.F. Simonds, and J.S. Gutkind. 1994. Ras-dependent activation of MAP kinase pathway mediated by G-protein beta gamma subunits. Nature 369: 418–420.CrossRefPubMed
42.
Remick, D.G., R.M. Strieter, M.K. Eskandari, D.T. Nguyen, M.A. Genord, C.L. Raiford, and S.L. Kunkel. 1990. Role of tumor necrosis factor-alpha in lipopolysaccharide-induced pathologic alterations. American Journal of Pathology 136: 49–60.PubMed
43.
Tessier, P.A., P.H. Naccache, I. Clark-Lewis, R.P. Gladue, K.S. Neote, and S.R. Mccoll. 1997. Chemokine networks in vivo: involvement of C–X–C and C–C chemokines in neutrophil extravasation in vivo in response to TNF-alpha. Journal of Immunology 159: 3595–3602.
44.
Wang, Y., and H. Thorlacius. 2005. Mast cell-derived tumour necrosis factor-alpha mediates macrophage inflammatory protein-2-induced recruitment of neutrophils in mice. British Journal of Pharmacology 145: 1062–1068.CrossRefPubMed
45.
Huang, S., J.D. Paulauskis, J.J. Godleski, and L. Kobzik. 1992. Expression of macrophage inflammatory protein-2 and KC mRNA in pulmonary inflammation. American Journal of Pathology 141: 981–988.PubMed
46.
Greenberger, M.J., R.M. Strieter, S.L. Kunkel, J.M. Danforth, L.L. Laichalk, D.C. Mcgillicuddy, and T.J. Standiford. 1996. Neutralization of macrophage inflammatory protein-2 attenuates neutrophil recruitment and bacterial clearance in murine Klebsiella pneumonia. Journal of Infectious Diseases 173: 159–165.PubMed
47.
Schmal, H., T.P. Shanley, M.L. Jones, H.P. Friedl, and P.A. Ward. 1996. Role for macrophage inflammatory protein-2 in lipopolysaccharide-induced lung injury in rats. Journal of Immunology 156: 1963–1972.
48.
Lomas-Neira, J.L., C.S. Chung, P.S. Grutkoski, E.J. Miller, and A. Ayala. 2004. CXCR2 inhibition suppresses hemorrhage-induced priming for acute lung injury in mice. Journal of Leukocyte Biology 76: 58–64.CrossRefPubMed
49.
Takano, K., K. Nakaima, M. Nitta, F. Shibata, and H. Nakagawa. 2004. Inhibitory effect of (−)-epigallocatechin 3-gallate, a polyphenol of green tea, on neutrophil chemotaxis in vitro and in vivo. Journal of Agricultural and Food Chemistry 52: 4571–4576.CrossRefPubMed
50.
Garbisa, S., L. Sartor, S. Biggin, B. Salvato, R. Benelli, and A. Albini. 2001. Tumor gelatinases and invasion inhibited by the green tea flavanol epigallocatechin-3-gallate. Cancer Letter 91: 822–832.
51.
Sartor, L., E. Pezzato, and S. Garbisa. 2002. (−)Epigallocatechin-3-gallate inhibits leukocyte elastase: Potential of the phyto-factor in hindering inflammation, emphysema, and invasion. Journal of Leukocyte Biology 71: 73–79.PubMed
Metadaten
Titel
The Effect of Epigallocatechin Gallate on Lipopolysaccharide-Induced Acute Lung Injury in a Murine Model
verfasst von
Hong-Beom Bae
Mei Li
Jong-Phil Kim
Seok-Jai Kim
Cheol-Won Jeong
Hyung-Gon Lee
Woong-Mo Kim
Hyung-Seok Kim
Sang-Hyun Kwak
Publikationsdatum
01.04.2010
Verlag
Springer US
Erschienen in
Inflammation / Ausgabe 2/2010
Print ISSN: 0360-3997
Elektronische ISSN: 1573-2576
DOI
https://doi.org/10.1007/s10753-009-9161-z

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