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01.05.2014 | Original Article

The efficacy of the use of IR laser phototherapy (LPT) on bone defect grafted with biphasic ceramic on rats with iron deficiency anemia: Raman spectroscopy analysis

verfasst von: Cristiane Becher Rosa, Isabele Cardoso Vieira de Castro, João Alves Reis Júnior, Juliana Silveira Aragão, Artur Felipe Santos Barbosa, Landulfo Silveira Jr, Antonio L. B. Pinheiro

Erschienen in: Lasers in Medical Science | Ausgabe 3/2014

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Abstract

The aim of this study was to evaluate bone repair in anemic and non-anemic rats submitted or not to laser phototherapy and hydroxyapatite graft. Animals were divided in eight groups of five animals: Clot; Laser; Graft; Graft + Laser; iron deficiency anemia (IDA) + Clot; IDA + Laser; IDA + graft; IDA + graft + Laser. When appropriate irradiation with infrared laser was done during 15 days at a 48-h interval. Animals were killed at day 30; samples were analyzed by Raman spectroscopy. Three shifts were studied and statistically analyzed: ~960, ~1,070, and ~1,454 cm⁻¹. Graft + laser showed highest ~960 peak was statistically different from all other healthy groups. No statistical difference was found between Clot and IDA + Clot in any shift. The IDA + Graft and IDA + Graft + Laser groups had low mean peak values for shifts ~960, ~1,070, and ~1,454 cm⁻¹. The results in this study indicate that using hydroxyapatite (HA) and laser irradiation in healthy subjects is favorable to mineral deposition and bone maturation, this being of importance for some groups at risk, such as astronauts. In iron deficiency anemia cases, the use of graft, associated or not to laser irradiation, resulted in low collagen and low carbonate and phosphate HA.

Protocol n^o05/10

Labina®, Purina, São Paulo Brazil

AIN93-G iron-free, São Paulo, Brazil

Vetaset®, São Paulo, SP, Brazil

Coopazine®, Cooper, Brazil

GenPhos® HATCP.BAUMER®; Mogi Mirim, São Paulo, Brazil

Eppendorf®, Brazil

Andor Technology, model Shamrock SR-303i®, Belfast, Northern Ireland

B&W TEK, model BRM-785-0.30-100-0.22.s, Newark, DE, USA

B&W TEK, model BAC-100-785, Newark, DE, USA

Andor Technology, model IDUs® DU401A-BR-DD, Belfast, Northern Ireland, 1,024 × 128 pixels, Peltier-cooled down to -70 °C

Andor Technology, Solis (i) software, Belfast, Northern Ireland

Intensity correction and wavenumber calibration.

Oriel Instruments, model 63358, Strattford, CT, USA

The Mathworks, Newark, NJ, USA

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Titel: The efficacy of the use of IR laser phototherapy (LPT) on bone defect grafted with biphasic ceramic on rats with iron deficiency anemia: Raman spectroscopy analysis
verfasst von: Cristiane Becher Rosa
Isabele Cardoso Vieira de Castro
João Alves Reis Júnior
Juliana Silveira Aragão
Artur Felipe Santos Barbosa
Landulfo Silveira Jr
Antonio L. B. Pinheiro
Publikationsdatum: 01.05.2014
Verlag: Springer London
Erschienen in: Lasers in Medical Science / Ausgabe 3/2014
Print ISSN: 0268-8921
Elektronische ISSN: 1435-604X
DOI: https://doi.org/10.1007/s10103-013-1496-x

Springer Medizin

Abstract

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