Background
Methods
Generation of stable EBNA1 HEK293T cells
Validation of EBNA1 expression by Western blot
Immunofluorescence of stably expressing EBNA1 HEK293T
High throughput RT-PCR screening of AS events
String network
Gene ontology analysis
Protein levels of splicing factors
RNA immunoprecipation
RIP-Seq library preparation
RIP-Seq analysis
Data availability
qPCR
As-PCR
Results
Expression of EBNA1 in HEK293T
EBNA1 modulation of AS events
Gene | ΔPSI | Gene | ΔPSI | Gene | ΔPSI |
---|---|---|---|---|---|
ABTB1
| 10.1 |
FSCN2
| −79.8 |
OPRL1
| −30.7 |
ADAMTSL1
| 25.4 |
GCNT1
| 25.4 |
OSBPL9
| −18.3 |
ALOX15B
| 76.1 |
GEM
| 12.8 |
OSCAR
| − 17.5 |
ANKMY1
| −13.7 |
GIGYF2
| −11.5 |
P2RX5
| 25.4 |
AOC2
| 12.9 |
GK
| −16.3 |
PAEP
| −69.3 |
ASAH2B
| 14.9 |
HGF
| 24.4 |
PAOX
| −73.8 |
ATG16L1
| −15.8 |
ICA1L
| −10.4 |
PAX2
| −15.6 |
ATP11A
| 15.7 |
IL37
| −38.6 |
PAXBP1
| −15.4 |
ATP6V1C2
| −10.0 |
IRF7
| 13.0 |
PCDH9
| 12.0 |
BMP4
| −14.7 |
ITGA6
| 13.9 |
PHKB
| 23.2 |
BRD8
| −20.1 |
ITGB1
| −35.5 |
PITX2
| 11.4 |
C16orf13
| −17.7 |
ITPR1
| 15.3 |
PKD1L2
| 19.0 |
C2orf88
| 76.4 |
IZUMO4
| 12.2 |
PLOD2
| 10.7 |
CAPN9
| 19.0 |
KIF25
| 42.0 |
PRC1
| −15.4 |
CCDC62
| −42.3 |
KIF9
| −19.4 |
PTP4A3
| −19.4 |
CD37
| 26.0 |
KL
| −28.8 |
PTPN13
| 15.2 |
CLK1
| 12.5 |
L3MBTL1
| 20.1 |
RBBP6
| −13.8 |
CLK2
| −18.3 |
LAMA4
| 10.2 |
RLN2
| −16.5 |
COL13A1
| 17.0 |
LETMD1
| 20.1 |
RNF135
| −24.7 |
COL8A1
| 11.2 |
LPPR5
| −23.2 |
RUNX2
| −16.3 |
CSPP1
| 15.6 |
MTMR3
| −13.3 |
SLC3A2
| −18.8 |
CTSB
| −24.5 |
MYH11
| −11.8 |
SYK
| 16.5 |
DCLRE1C
| −15.9 |
MYO18A
| −16.1 |
TM2D2
| −14.4 |
DLEC1
| −53.8 |
NAA60
| 11.7 |
TMTC4
| 13.3 |
DMBX1
| −13.8 |
NDEL1
| −11.2 |
TRPV4
| −18.3 |
DTX2
| −11.0 |
NFATC2
| 18.5 |
TSGA10
| 14.9 |
EFNA1
| −13.0 |
NFU1
| −12.0 |
TTC23
| −12.7 |
EPS8L3
| 13.8 |
NRXN2
| −11.8 |
ZNF493
| 25.0 |
FAM13B
| 20.4 |
NTRK2
| 41.0 |
ZRANB2
| −11.5 |
FAM86A
| 28.6 |
ODF2L
| −13.1 |