Treadmill distance (m) was used to assess physical performance and was recorded via a computerised program (Treadmill Measuring System Version 2.0, UWA, Australia) with data averaged every 30 min. The treadmill display board (speed and distance) was hidden from participant viewing to ensure condition blinding.
Six hours prior to arrival, participants ingested a radiotelemetry pill to measure gastrointestinal T
c (CorTemp, HQ Inc, USA). On arrival and at the end of the protocol, a mid-stream urine sample was collected to determine USG (Kavouras,
2002), using a desktop refractometer (Model T3-NE, Atago Co. Ltd., Japan) to establish hydration status. Nude body mass was then measured to the nearest 0.05 kg using a digital platform (Model ED3300; Sauter Multi-Range Ebingen, Germany); this was also repeated immediately after the protocol (towel dried) for the calculation of sweat loss (pre – post nude body mass + food + water intake + urinary output). A HR monitor (Polar RS400, Finland) was fitted around the participant’s chest, and thermistors (Skin Sensor SST-1, Physitemp Instruments Inc, NJ, USA) were taped to three sites: sternum, right medial forearm, and right mid-posterior calf to determine mean T
sk via a computerised program (DASYLab Light, National Instruments, Ireland Resources Ltd.). Mean T
sk was calculated using the formula of Burton (
1935): T
sk = (0.5 × sternum temperature) + (0.14 × forearm temperature) + (0.36 × calf temperature). Measures of TS and TC were obtained every 5 min during the protocol on visual analog scales ranging from green to red scale (0 = ‘very cold’ to 20 = ‘very hot’) and from white to black graduation (0 = ‘very comfortable’ to 20 = ‘very uncomfortable’)
, respectively (Gaoua et al.
2012). These physiological (T
c, T
sk and HR) and perceptual (TS and TC) measures were obtained at baseline and every 5 min thereafter, with the exception of T
sk which was not recorded during rest breaks.
Short-term working memory was assessed using the counting span task (Case et al.
1982; Millisecond Software, Seattle, USA), with assessments made upon entering the climate chamber and after every 60 min of simulated work. Briefly, participants were presented with a number of cards (‘spans’) which included yellow and green dots. They were required to remember the number of green dots presented on each card in the order of presentation within the span and then to recall this information later. Counting span performance was recorded as the highest level in which participants successfully recalled the spans, plus one-third of a point for each span recalled at a higher level (Case et al.
1982).
Perceived fatigue was assessed before and after the treadmill exercise using the Multidimensional Fatigue Inventory (Smets et al.
1995). This questionnaire measures the sub-dimensions of general, mental and physical fatigue, as well as reduced motivation and reduced activity. The Multidimensional Fatigue Inventory has displayed good internal consistency between general, physical and mental fatigue sub-dimensions (Cronbach’s alpha > 0.80) and adequate reliability for reduced activity and reduced motivation sub-dimensions (Cronbach’s alpha > 0.65) (Lin et al.
2009). Test–retest reliability had also been reported to be between 0.59 and 0.68 for each of the sub-dimensions of perceived fatigue (Chung et al.
2014).