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Archives of Gynecology and Obstetrics

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16.06.2022 | Maternal-Fetal Medicine

The investigation of hippo signaling pathway in mouse uterus during peri-implantation period

verfasst von: Ezgi Golal, Cemre Nur Balci, Ismail Ustunel, Nuray Acar

Erschienen in: Archives of Gynecology and Obstetrics | Ausgabe 6/2023

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Abstract

Purpose

Events in the uterus during the peri-implantation period include embryo development, acquisition of uterine receptivity, implantation and decidualization. Hippo signaling pathway regulates cell proliferation, apoptosis and differentiation. We aimed to determine localization and expressions of pYAP (Phospho Yes-associated protein), YAP (Yes-associated protein), TEAD1 (TEA domain family member 1) and CTGF (Connective tissue growth factor), members of the Hippo signaling pathway, in the mouse uterus during the peri-implantation period.

Methods

Pregnant mice were randomly separated into 5 groups: 1st, 4th, 5th, 6th, and 8th days of pregnancy groups. Non-pregnant female mice in estrous phase were included in the estrous group. Uteri and implantation sites were collected. Also, inter-implantation sites were collected from the 5th day of pregnancy group. pYAP, YAP, TEAD-1 and CTGF were detected by immunohistochemistry and Western blotting.

Results

We observed that the expressions of YAP, TEAD-1 and CTGF were increased in the luminal and glandular epithelium on the 1st and 4th days of pregnancy when epithelial proliferation occurred. pYAP expression was high, and YAP and CTGF expressions were low in the luminal epithelium of the implantation sites on the 5th day of pregnancy, when epithelial differentiation occurred. pYAP expression was low, YAP and CTGF expressions were high at implantation sites on the 6th and 8th days of pregnancy, where decidua was formed.

Conclusion

Our findings suggest that the Hippo signaling pathway might be involved in implantation and decidualization. Our findings will guide further studies and may help to elucidate underlying causes of implantation failure and pregnancy loss.

Paria BC, Song H, Dey SK (2001) Implantation: molecular basis of embryo-uterine dialogue. Int J Dev Biol 45(3):597–605PubMed

Tu Z et al (2014) Molecular determinants of uterine receptivity. Int J Dev Biol 58(2–4):147–154. https://doi.org/10.1387/ijdb.130345whCrossRefPubMed

Wang H, Dey SK (2006) Roadmap to embryo implantation: clues from mouse models. Nat Rev Genet 7(3):185–199. https://doi.org/10.1038/nrg1808CrossRefPubMed

Zhang S, Lin H, Kong S, Wang S, Wang H, Wang H, Armant DR (2013) Physiological and molecular determinants of embryo implantation. Mol Aspects Med 34(5):939–980. https://doi.org/10.1016/j.mam.2012.12.011CrossRefPubMedPubMedCentral

Namiki T, Ito J, Kashiwazaki N (2018) Molecular mechanisms of embryonic implantation in mammals: lessons from the gene manipulation of mice. Reprod Med Biol 17(4):331–342. https://doi.org/10.1002/rmb2.12103CrossRefPubMedPubMedCentral

Tu Z, Wang Q, Cui T, Wang J, Ran H, Bao H, Lu J, Wang B, Lydon JP, DeMayo F, Zhang S, Kong S, Wu X, Wang H (2016) Uterine RAC1 via Pak1-ERM signaling directs normal luminal epithelial integrity conducive to on-time embryo implantation in mice. Cell Death Differ 23(1):169–181. https://doi.org/10.1038/cdd.2015.98CrossRefPubMed

Bao Y, Nakagawa K, Yang Z, Ikeda M, Withanage K, Ishigami-Yuasa M, Okuno Y, Hata S, Nishina H, Hata Y (2011) A cell-based assay to screen stimulators of the Hippo pathway reveals the inhibitory effect of dobutamine on the YAP-dependent gene transcription. J Biochem 150(2):199–208. https://doi.org/10.1093/jb/mvr063CrossRefPubMed

Dong J, Feldmann G, Huang J, Wu S, Zhang N, Comerford SA, Gayyed MF, Anders RA, Maitra A, Pan D (2007) Elucidation of a universal size-control mechanism in Drosophila and mammals. Cell 130(6):1120–1133. https://doi.org/10.1016/j.cell.2007.07.019CrossRefPubMedPubMedCentral

Zhao B, Wei X, Li W, Udan RS, Yang Q, Kim J, Xie J, Ikenoue T, Yu J, Li L, Zheng P, Ye K, Chinnaiyan A, Halder G, Lai ZC, Guan KL (2007) Inactivation of YAP oncoprotein by the Hippo pathway is involved in cell contact inhibition and tissue growth control. Genes Dev 21(21):2747–2761. https://doi.org/10.1101/gad.1602907CrossRefPubMedPubMedCentral

10.

Yu FX, Guan KL (2013) The Hippo pathway: regulators and regulations. Genes Dev 27(4):355–371. https://doi.org/10.1101/gad.210773.112CrossRefPubMedPubMedCentral

11.

Camargo FD, Gokhale S, Johnnidis JB, Fu D, Bell GW, Jaenisch R, Brummelkamp TR (2007) YAP1 increases organ size and expands undifferentiated progenitor cells. Curr Biol 17(23):2054–2060. https://doi.org/10.1016/j.cub.2007.10.039CrossRefPubMed

12.

Cherrett C, Furutani-Seiki M, Bagby S (2012) The Hippo pathway: key interaction and catalytic domains in organ growth control, stem cell self-renewal and tissue regeneration. Essays Biochem 53:111–127. https://doi.org/10.1042/bse0530111CrossRefPubMed

13.

Zhang H, Pasolli HA, Fuchs E (2011) Yes-associated protein (YAP) transcriptional coactivator functions in balancing growth and differentiation in skin. Proc Natl Acad Sci U S A 108(6):2270–2275. https://doi.org/10.1073/pnas.1019603108CrossRefPubMedPubMedCentral

14.

Kaneko KJ, DePamphilis ML (1998) Regulation of gene expression at the beginning of mammalian development and the TEAD family of transcription factors. Dev Genet 22(1):43–55. https://doi.org/10.1002/(SICI)1520-6408(1998)22:1%3c43::AID-DVG5%3e3.0.CO;2-7CrossRefPubMed

15.

Zhao B, Lei QY, Guan KL (2008) The Hippo-YAP pathway: new connections between regulation of organ size and cancer. Curr Opin Cell Biol 20(6):638–646. https://doi.org/10.1016/j.ceb.2008.10.001CrossRefPubMedPubMedCentral

16.

Vassilev A, Kaneko KJ, Shu H, Zhao Y, DePamphilis ML (2001) TEAD/TEF transcription factors utilize the activation domain of YAP65, a Src/Yes-associated protein localized in the cytoplasm. Genes Dev 15(10):1229–1241. https://doi.org/10.1101/gad.888601CrossRefPubMedPubMedCentral

17.

Thongon N, Castiglioni I, Zucal C, Latorre E, D’Agostino V, Bauer I, Pancher M, Ballestrero A, Feldmann G, Nencioni A, Provenzani A (2016) The GSK3beta inhibitor BIS I reverts YAP-dependent EMT signature in PDAC cell lines by decreasing SMADs expression level. Oncotarget 7(18):26551–26566. https://doi.org/10.18632/oncotarget.8437CrossRefPubMedPubMedCentral

18.

Zhao B, Ye X, Yu J, Li L, Li W, Li S, Yu J, Lin JD, Wang CY, Chinnaiyan AM, Lai ZC, Guan KL (2008) TEAD mediates YAP-dependent gene induction and growth control. Genes Dev 22(14):1962–1971. https://doi.org/10.1101/gad.1664408CrossRefPubMedPubMedCentral

19.

Yang N, Morrison CD, Liu P, Miecznikowski J, Bshara W, Han S, Zhu Q, Omilian AR, Li X, Zhang J (2012) TAZ induces growth factor-independent proliferation through activation of EGFR ligand amphiregulin. Cell Cycle 11(15):2922–2930. https://doi.org/10.4161/cc.21386CrossRefPubMedPubMedCentral

20.

Bradham DM, Igarashi A, Potter RL, Grotendorst GR (1991) Connective tissue growth factor: a cysteine-rich mitogen secreted by human vascular endothelial cells is related to the SRC-induced immediate early gene product CEF-10. J Cell Biol 114(6):1285–1294. https://doi.org/10.1083/jcb.114.6.1285CrossRefPubMed

21.

Oemar BS, Luscher TF (1997) Connective tissue growth factor. Friend or foe? Arterioscler Thromb Vasc Biol 17(8):1483–1489. https://doi.org/10.1161/01.atv.17.8.1483CrossRefPubMed

22.

Igarashi A, Nashiro K, Kikuchi K, Sato S, Ihn H, Grotendorst GR, Takehara K (1995) Significant correlation between connective tissue growth factor gene expression and skin sclerosis in tissue sections from patients with systemic sclerosis. J Invest Dermatol 105(2):280–284. https://doi.org/10.1111/1523-1747.ep12318465CrossRefPubMed

23.

Igarashi A, Nashiro K, Kikuchi K, Sato S, Ihn H, Fujimoto M, Grotendorst GR, Takehara K (1996) Connective tissue growth factor gene expression in tissue sections from localized scleroderma, keloid, and other fibrotic skin disorders. J Invest Dermatol 106(4):729–733. https://doi.org/10.1111/1523-1747.ep12345771CrossRefPubMed

24.

Frazier KS, Grotendorst GR (1997) Expression of connective tissue growth factor mRNA in the fibrous stroma of mammary tumors. Int J Biochem Cell Biol 29(1):153–161. https://doi.org/10.1016/s1357-2725(96)00127-6CrossRefPubMed

25.

Allen JT, Knight RA, Bloor CA, Spiteri MA (1999) Enhanced insulin-like growth factor binding protein-related protein 2 (Connective tissue growth factor) expression in patients with idiopathic pulmonary fibrosis and pulmonary sarcoidosis. Am J Respir Cell Mol Biol 21(6):693–700. https://doi.org/10.1165/ajrcmb.21.6.3719CrossRefPubMed

26.

Ito Y, Aten J, Bende RJ, Oemar BS, Rabelink TJ, Weening JJ, Goldschmeding R (1998) Expression of connective tissue growth factor in human renal fibrosis. Kidney Int 53(4):853–861. https://doi.org/10.1111/j.1523-1755.1998.00820.xCrossRefPubMed

27.

Ball DK, Surveyor GA, Diehl JR, Steffen CL, Uzumcu M, Mirando MA, Brigstock DR (1998) Characterization of 16- to 20-kilodalton (kDa) connective tissue growth factors (CTGFs) and demonstration of proteolytic activity for 38-kDa CTGF in pig uterine luminal flushings. Biol Reprod 59(4):828–835. https://doi.org/10.1095/biolreprod59.4.828CrossRefPubMed

28.

Brigstock DR, Steffen CL, Kim GY, Vegunta RK, Diehl JR, Harding PA (1997) Purification and characterization of novel heparin-binding growth factors in uterine secretory fluids. Identification as heparin-regulated Mr 10,000 forms of connective tissue growth factor. J Biol Chem 272(32):20275–20282. https://doi.org/10.1074/jbc.272.32.20275CrossRefPubMed

29.

Frazier K, Williams S, Kothapalli D, Klapper H, Grotendorst GR (1996) Stimulation of fibroblast cell growth, matrix production, and granulation tissue formation by connective tissue growth factor. J Invest Dermatol 107(3):404–411. https://doi.org/10.1111/1523-1747.ep12363389CrossRefPubMed

30.

Igarashi A, Okochi H, Bradham DM, Grotendorst GR (1993) Regulation of connective tissue growth factor gene expression in human skin fibroblasts and during wound repair. Mol Biol Cell 4(6):637–645. https://doi.org/10.1091/mbc.4.6.637CrossRefPubMedPubMedCentral

31.

Kireeva ML, Latinkic BV, Kolesnikova TV, Chen CC, Yang GP, Abler AS, Lau LF (1997) Cyr61 and Fisp12 are both ECM-associated signaling molecules: activities, metabolism, and localization during development. Exp Cell Res 233(1):63–77. https://doi.org/10.1006/excr.1997.3548CrossRefPubMed

32.

Steffen CL, Ball-Mirth DK, Harding PA, Bhattacharyya N, Pillai S, Brigstock DR (1998) Characterization of cell-associated and soluble forms of connective tissue growth factor (CTGF) produced by fibroblast cells in vitro. Growth Factors 15(3):199–213. https://doi.org/10.3109/08977199809002117CrossRefPubMed

33.

Hishikawa K, Oemar BS, Tanner FC, Nakaki T, Fujii T, Luscher TF (1999) Overexpression of connective tissue growth factor gene induces apoptosis in human aortic smooth muscle cells. Circulation 100(20):2108–2112. https://doi.org/10.1161/01.cir.100.20.2108CrossRefPubMed

34.

Nagashima T, Kim J, Li Q, Lydon JP, DeMayo FJ, Lyons KM, Matzuk MM (2011) Connective tissue growth factor is required for normal follicle development and ovulation. Mol Endocrinol 25(10):1740–1759. https://doi.org/10.1210/me.2011-1045CrossRefPubMedPubMedCentral

35.

Hashimoto M, Sasaki H (2019) Epiblast formation by TEAD-YAP-dependent expression of pluripotency factors and competitive elimination of unspecified cells. Dev Cell 50(2):139-154 e5. https://doi.org/10.1016/j.devcel.2019.05.024CrossRefPubMed

36.

Karasek C, Ashry M, Driscoll CS, Knott JG (2020) A tale of two cell-fates: role of the Hippo signaling pathway and transcription factors in early lineage formation in mouse preimplantation embryos. Mol Hum Reprod 26(9):653–664. https://doi.org/10.1093/molehr/gaaa052CrossRefPubMedPubMedCentral

37.

Meinhardt G et al (2020) Pivotal role of the transcriptional co-activator YAP in trophoblast stemness of the developing human placenta. Proc Natl Acad Sci U S A 117(24):13562–13570. https://doi.org/10.1073/pnas.2002630117CrossRefPubMedPubMedCentral

38.

Ramathal CY, Bagchi IC, Taylor RN, Bagchi MK (2010) Endometrial decidualization: of mice and men. Semin Reprod Med 28(1):17–26. https://doi.org/10.1055/s-0029-1242989CrossRefPubMedPubMedCentral

39.

Chen H, Song Y, Yang S, Fu J, Feng X, Huang W (2017) YAP mediates human decidualization of the uterine endometrial stromal cells. Placenta 53:30–35. https://doi.org/10.1016/j.placenta.2017.03.013CrossRefPubMed

40.

Song Y, Fu J, Zhou M, Xiao L, Feng X, Chen H, Huang W (2016) Activated hippo/yes-associated protein pathway promotes cell proliferation and anti-apoptosis in endometrial stromal cells of endometriosis. J Clin Endocrinol Metab 101(4):1552–1561. https://doi.org/10.1210/jc.2016-1120CrossRefPubMedPubMedCentral

41.

Pei T et al (2019) Increased expression of YAP is associated with decreased cell autophagy in the eutopic endometrial stromal cells of endometriosis. Mol Cell Endocrinol 491:110432. https://doi.org/10.1016/j.mce.2019.04.012CrossRefPubMed

42.

Tsai K et al (2021) Differential expression of mTOR related molecules in the placenta from gestational diabetes mellitus (GDM), intrauterine growth restriction (IUGR) and preeclampsia patients. Reprod Biol 21(2):100503. https://doi.org/10.1016/j.repbio.2021.100503CrossRefPubMed

43.

Sun M et al (2018) YAP is decreased in preeclampsia and regulates invasion and apoptosis of HTR-8/SVneo. Reprod Sci 25(9):1382–1393. https://doi.org/10.1177/1933719117746784CrossRefPubMed

44.

Saha B et al (2020) TEAD4 ensures postimplantation development by promoting trophoblast self-renewal: an implication in early human pregnancy loss. Proc Natl Acad Sci U S A 117(30):17864–17875. https://doi.org/10.1073/pnas.2002449117CrossRefPubMedPubMedCentral

45.

Paria BC, Huet-Hudson YM, Dey SK (1993) Blastocyst’s state of activity determines the “window” of implantation in the receptive mouse uterus. Proc Natl Acad Sci U S A 90(21):10159–10162. https://doi.org/10.1073/pnas.90.21.10159CrossRefPubMedPubMedCentral

46.

Song H, Lim H, Paria BC, Matsumoto H, Swift LL, Morrow J, Bonventre JV, Dey SK (2002) Cytosolic phospholipase A2alpha is crucial [correction of A2alpha deficiency is crucial] for “on-time” embryo implantation that directs subsequent development. Development 129(12):2879–2889CrossRefPubMed

47.

Acar N, Soylu H, Edizer I, Ozbey O, Er H, Akkoyunlu G, Gemici B, Ustunel I (2014) Expression of nuclear factor erythroid 2-related factor 2 (Nrf2) and peroxiredoxin 6 (Prdx6) proteins in healthy and pathologic placentas of human and rat. Acta Histochem 116(8):1289–1300. https://doi.org/10.1016/j.acthis.2014.07.012CrossRefPubMed

48.

Acar N, Korgun ET, Ustunel I (2012) Cell cycle inhibitor p57 expression in normal and diabetic rat placentas during some stages of pregnancy. Histol Histopathol 27(1):59–68. https://doi.org/10.14670/HH-27.59CrossRefPubMed

49.

Cha J, Sun X, Dey SK (2012) Mechanisms of implantation: strategies for successful pregnancy. Nat Med 18(12):1754–1767. https://doi.org/10.1038/nm.3012CrossRefPubMedPubMedCentral

50.

Moon S et al (2019) STK3/4 expression is regulated in uterine endometrial cells during the estrous cycle. Cells. https://doi.org/10.3390/cells8121643CrossRefPubMedPubMedCentral

51.

Wood GA, Fata JE, Watson KL, Khokha R (2007) Circulating hormones and estrous stage predict cellular and stromal remodeling in murine uterus. Reproduction 133(5):1035–1044. https://doi.org/10.1530/REP-06-0302CrossRefPubMed

52.

Huet-Hudson YM, Andrews GK, Dey SK (1989) Cell type-specific localization of c-myc protein in the mouse uterus: modulation by steroid hormones and analysis of the periimplantation period. Endocrinology 125(3):1683–1690. https://doi.org/10.1210/endo-125-3-1683CrossRefPubMed

53.

Yuan J, Aikawa S, Deng W, Bartos A, Walz G, Grahammer F, Huber TB, Sun X, Dey SK (2019) Primary decidual zone formation requires Scribble for pregnancy success in mice. Nat Commun 10(1):5425. https://doi.org/10.1038/s41467-019-13489-4CrossRefPubMedPubMedCentral

54.

Li Y, Sun X, Dey SK (2015) Entosis allows timely elimination of the luminal epithelial barrier for embryo implantation. Cell Rep 11(3):358–365. https://doi.org/10.1016/j.celrep.2015.03.035CrossRefPubMedPubMedCentral

55.

Ota M, Sasaki H (2008) Mammalian Tead proteins regulate cell proliferation and contact inhibition as transcriptional mediators of Hippo signaling. Development 135(24):4059–4069. https://doi.org/10.1242/dev.027151CrossRefPubMed

56.

Landin Malt A, Cagliero J, Legent K, Silber J, Zider A, Flagiello D (2012) Alteration of tead1 expression levels confers apoptotic resistance through the transcriptional up-regulation of livin. PLoS ONE 7(9):e45498. https://doi.org/10.1371/journal.pone.0045498CrossRefPubMedPubMedCentral

57.

Osman I, He X, Liu J, Dong K, Wen T, Zhang F, Yu L, Hu G, Xin H, Zhang W, Zhou J (2019) TEAD1 (TEA domain transcription factor 1) promotes smooth muscle cell proliferation through upregulating SLC1A5 (solute carrier family 1 member 5)-mediated glutamine uptake. Circ Res 124(9):1309–1322. https://doi.org/10.1161/CIRCRESAHA.118.314187CrossRefPubMedPubMedCentral

58.

Paria BC, Zhao X, Das SK, Dey SK, Yoshinaga K (1999) Zonula occludens-1 and E-cadherin are coordinately expressed in the mouse uterus with the initiation of implantation and decidualization. Dev Biol 208(2):488–501. https://doi.org/10.1006/dbio.1999.9206CrossRefPubMed

59.

Tan J, Raja S, Davis MK, Tawfik O, Dey SK, Das SK (2002) Evidence for coordinated interaction of cyclin D3 with p21 and cdk6 in directing the development of uterine stromal cell decidualization and polyploidy during implantation. Mech Dev 111(1–2):99–113. https://doi.org/10.1016/s0925-4773(01)00614-1CrossRefPubMedPubMedCentral

60.

Rageh MA, Moussad EE, Wilson AK, Brigstock DR (2001) Steroidal regulation of connective tissue growth factor (CCN2; CTGF) synthesis in the mouse uterus. Mol Pathol 54(5):338–346. https://doi.org/10.1136/mp.54.5.338CrossRefPubMedPubMedCentral

61.

Tranguch S, Smith DF, Dey SK (2006) Progesterone receptor requires a co-chaperone for signalling in uterine biology and implantation. Reprod Biomed Online 13(5):651–660. https://doi.org/10.1016/s1472-6483(10)60655-4CrossRefPubMed

62.

Brigstock DR (2002) Regulation of angiogenesis and endothelial cell function by connective tissue growth factor (CTGF) and cysteine-rich 61 (CYR61). Angiogenesis 5(3):153–165. https://doi.org/10.1023/a:1023823803510CrossRefPubMed

63.

Ma W, Tan J, Matsumoto H, Robert B, Abrahamson DR, Das SK, Dey SK (2001) Adult tissue angiogenesis: evidence for negative regulation by estrogen in the uterus. Mol Endocrinol 15(11):1983–1992. https://doi.org/10.1210/mend.15.11.0734CrossRefPubMed

Titel: The investigation of hippo signaling pathway in mouse uterus during peri-implantation period
verfasst von: Ezgi Golal
Cemre Nur Balci
Ismail Ustunel
Nuray Acar
Publikationsdatum: 16.06.2022
Verlag: Springer Berlin Heidelberg
Erschienen in: Archives of Gynecology and Obstetrics / Ausgabe 6/2023
Print ISSN: 0932-0067
Elektronische ISSN: 1432-0711
DOI: https://doi.org/10.1007/s00404-022-06660-8

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The investigation of hippo signaling pathway in mouse uterus during peri-implantation period

Abstract

Purpose

Methods

Results

Conclusion

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Purpose

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