Introduction
Methods
General Methodology
Cells and Culturing
SkQ1 Stock and Working Solutions
Cell Viability, Dosing and Safety Ranges
Cell Viability Determination with Varying Concentrations of SkQ1 and Diluent
Cell Viability Determination when SkQ1 Is Combined with Sensitizing Reagent IL-1β or TNF-α
Role of SkQ1 in Inflammation of HCjE Conjunctival Epithelial Cells
SkQ1 Treatment of HCjE Inflammation
PGE2 Assay
Role of SkQ1 in Corneal Wound Healing
Wound Creation
SkQ1 Treatment Groups
End Point Measurements of the Corneal Wound-healing Process
Cell Proliferation
Cell Migration
Data Analysis
Evaluation of Inflammation on HCjE
Evaluation of Wound Healing on HCLE
Results
Cell Viability, Dosing and Safety Ranges
Cell Viability Determination with Varying Concentrations of SkQ1 and Diluent
Cell Viability Determination when SkQ1 Is Combined with Sensitizing Reagent IL-1β or TNF-α
Role of SkQ1 in Inflammation of HCjE Conjunctival Epithelial Cells
Role of SkQ1 in HCLE Wound Healing
SkQ1 in Wound Healing in HCLE Cell Cultures
Time-points (hours after scratching) | SKQ1 at 0 nM | SKQ1 at 50 nM | p valueb | ||
---|---|---|---|---|---|
Meana ± σ (%) |
n
| Mean ± σ (%) |
n
| ||
0 | 0 ± 0 | 11 | 0 ± 0 | 11 | 1 |
4 | 17 ± 6 | 11 | 21 ± 5 | 11 | 0.015* |
8 | 53 ± 13 | 11 | 62 ± 10 | 11 | 0.007** |
12 | 89 ± 10 | 11 | 98 ± 4 | 11 | 0.003** |